LOCUS       pJH53_pE12_hyCal        3974 bp ds-DNA     circular     10-SEP-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Insert from 710520-3 15 to 1471
COMMENT     Backbone from pJH19_pDONR221 2897 to 651
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(2235..2255)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="forestgreen"
                     /ApEinfo_revcolor="dodgerblue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="forestgreen"
                     /ApEinfo_revcolor="dodgerblue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     570..651
                     /locus_tag="AttP1"
                     /label="AttP1"
                     /ApEinfo_label="AttP1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     570..651
                     /locus_tag="AttL1"
                     /label="AttL1"
                     /ApEinfo_label="AttL1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2215..2236)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="forestgreen"
                     /ApEinfo_revcolor="dodgerblue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2368..3174
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2109..2122)
                     /locus_tag="AttB2"
                     /label="AttB2"
                     /ApEinfo_label="AttB2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(2109..2197)
                     /locus_tag="AttP2"
                     /label="AttP2"
                     /ApEinfo_label="AttP2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(2109..2197)
                     /locus_tag="AttL2"
                     /label="AttL2"
                     /ApEinfo_label="AttL2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(2109..2122)
                     /locus_tag="AttR2"
                     /label="AttR2"
                     /ApEinfo_label="AttR2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    645..651
                     /locus_tag="AttB1"
                     /label="AttB1"
                     /ApEinfo_label="AttB1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2109..2115)
                     /locus_tag="Gateway Recomb2"
                     /label="Gateway Recomb2"
                     /ApEinfo_label="Gateway Recomb2"
                     /ApEinfo_fwdcolor="red"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     645..651
                     /locus_tag="AttR1"
                     /label="AttR1"
                     /ApEinfo_label="AttR1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             683..2092
                     /locus_tag="hyNaC2"
                     /label="hyNaC2"
                     /ApEinfo_label="hyNaC2"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTA GCTAGCGCCA CCATGAAGGC TAGATTCGCC AGAAGAATGG AGAAAATCGT
      721 GGAGGAAAGA ATGACCGTGA AACAGATTTT TAATAGATAC GTGCAGACCT CTACCCTGCA
      781 CGGATTTCGG TTCATCTTCA TGGATACCTT CATTGTGAGA AGAGTGCTGT GGACAATCCT
      841 GACCCTGACA ATGGCTACAA TTTTCTTTAA GGAACTGAGA AATTCCATCA ATCTGTTCTA
      901 TGAGTATCCA TTCACAACAA CCAGCACCAT CCAGTACGAA CCATCTCTGA CATTCCCAGC
      961 TATTAGCGTG TGTAATCTGA ATCACTTTCT GCTGAGCAAA ATCAAGAAGA GCAAACTGAA
     1021 GCCACTGTAT GATCAGGGAA GACTGCCATT TGACAACAAT CTGGAGAATC CAGGATTCGA
     1081 TATTCAGGGA GAGGAGCTGT ACAGCATTCT GAAGACAAGC TCTCAGAGCA TTGATGAAAT
     1141 TTTTCTGTCT TGCGAATGGA AAAGCAGAGA CACAGCCAAG AATGGAGTGC CAAATCCATG
     1201 CAAACCAAAC AACTTCACAG TGTACAGCGG ACTGTACGGA CAGAGCTGCT ACACATTCAA
     1261 TCCAGGAGTG AGCGGATATC CACTGCTGTC TCTGAGCGAA ACCGGAGTGA ACATGGGATT
     1321 CAAGCTGGAG CTGGATCTGA AGTCTAAGGA CTCTCTGCAG GGAATTCAGG AGATTGGAGC
     1381 TATCGTGATC GTGCATCACC AGCAGGAGAC CCCAGTGCTG CAGGCCGGAT TCGTGGTGTC
     1441 TCCAGGATTC CAGACATTCG TGGAGATTAA GGTGAGACAG ACCGAAAATC TGCCACCACC
     1501 ATATGCTACA AAATGCGGAT CTAAACCACT GAAGAACTAT CAGATCTACA GACAGTCTAG
     1561 CTGCTTTCTG GAACAGCTGG GAGATGCCAT TGAAACAAAG TGCAAATGCA AGAGCTCTTT
     1621 TATGGCTGGA AGAAATATCC CATACTGCTC TCTGAGACAG ACCGTGACAT GTCTGATGCC
     1681 AACCATCTAC GACTTTGACA GAAAGACAAA TAACAATTGC CCAGTGGACT GTGAAACAAT
     1741 TCAGTACCTG TCTAGCCTGT CTTACGCTAG ATTCATTAGC AATGTGACAT ACCTGAGCAA
     1801 GAACGCTGAA CAGAGCAGCT ACATTAGAAA ACTGAAAAAT AGCATGTCCC CAAAAAAGCT
     1861 GCAGAAGTAT ATCGAAGAAA ATATTGTGGC TGTGCAGTTC TTTTACCAGG AGATGAAGAA
     1921 GGAAAAGGTG AAACAGGAGC CAAGCTACGA TTTTTATAAA CTGATTGGAG ACGTGGGAGG
     1981 ACAGCTGGGA CTGCTGCTGG GAGCCTCTGT GCTGACACTG GTGGAGTTTG TGGATCTGTT
     2041 CATTTTTACC CTGTACCACC AGCTGCTGAG ACTGAGCAAA AAGAAGTCAT GAGGATCCAC
     2101 CCAGCTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC
     2161 GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT
     2221 AGTGAGTCGT ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT
     2281 CTCTGATGTT ACATTGCACA AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT
     2341 TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG
     2401 CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC
     2461 GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT
     2521 CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC
     2581 TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT
     2641 GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT
     2701 CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG
     2761 ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA
     2821 TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG
     2881 CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC
     2941 GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT
     3001 TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG
     3061 AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT
     3121 GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA
     3181 GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA
     3241 AGCTCATGAC CAAAATCCCT TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC
     3301 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG
     3361 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT
     3421 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG
     3481 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG
     3541 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC
     3601 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA
     3661 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA
     3721 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC
     3781 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT
     3841 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG
     3901 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT
     3961 TTTGCTCACA TGTT    
//
