LOCUS       pJH201_pE12_hyCa        4963 bp ds-DNA     circular     10-SEP-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Insert from 710520-4 15 to 1576
COMMENT     Backbone from pJH19_pDONR221 2897 to 651
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(3224..3244)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="forestgreen"
                     /ApEinfo_revcolor="dodgerblue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="forestgreen"
                     /ApEinfo_revcolor="dodgerblue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     570..651
                     /locus_tag="AttP1"
                     /label="AttP1"
                     /ApEinfo_label="AttP1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     570..651
                     /locus_tag="AttL1"
                     /label="AttL1"
                     /ApEinfo_label="AttL1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3204..3225)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="forestgreen"
                     /ApEinfo_revcolor="dodgerblue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3357..4163
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3098..3111)
                     /locus_tag="AttB2"
                     /label="AttB2"
                     /ApEinfo_label="AttB2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(3098..3186)
                     /locus_tag="AttP2"
                     /label="AttP2"
                     /ApEinfo_label="AttP2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(3098..3186)
                     /locus_tag="AttL2"
                     /label="AttL2"
                     /ApEinfo_label="AttL2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(3098..3111)
                     /locus_tag="AttR2"
                     /label="AttR2"
                     /ApEinfo_label="AttR2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    645..651
                     /locus_tag="AttB1"
                     /label="AttB1"
                     /ApEinfo_label="AttB1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(2237..2389,2441..2634,2686..2878,2930..3094)
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#339933"
                     /ApEinfo_revcolor="#339933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(3098..3104)
                     /locus_tag="Gateway Recomb2"
                     /label="Gateway Recomb2"
                     /ApEinfo_label="Gateway Recomb2"
                     /ApEinfo_fwdcolor="red"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     645..651
                     /locus_tag="AttR1"
                     /label="AttR1"
                     /ApEinfo_label="AttR1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            2225..2233
                     /locus_tag="mCherry(1)"
                     /label="mCherry(1)"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             683..2194
                     /locus_tag="hyNaC7"
                     /label="hyNaC7"
                     /ApEinfo_label="hyNaC7"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2195..2224
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTA GCTAGCGCCA CCATGAGAAT CAGACTGAAG GAGAGAATGA GAAAAATTGT
      721 GCAGGAAAGA CTGACCGTGA AACAGATTTT TAAAAGATAT ATCGAGAGCT CTACCCTGCA
      781 CGGATTTTGT TATGTGTGTA TGGACACATT CCTGGGAAGA AGACTGATTT GGGCCGTGCT
      841 GATGATCCTG GGAGCCATCT ATTTCATTTT TAAACTGAGA TACGGCATCA AGGAATACTT
      901 TGACTATCCA TTCAGCACCC TGTCTACAGT GGAATATGTG GACGATCTGC TGTTTCCAGC
      961 CGTGTCTGTG TGCGCTACCA ATAGCTACAT TGCCTCTCAG GTGTATACCA ATCAGCTGAA
     1021 TACAATGTAT AAGGAGGGAA GACTGCCACT GGATAACAAC CAGTCTATCC CAGAATATAA
     1081 CATTCCAGGA GACGAGCTGG TGAAGACCCT GAAGAACAGC AGCCTGACAA TTGAGTCTCT
     1141 GCTGAAGTAT TGTGACTGGA TCATGCAGGA CACATCTCAC CCACTGGTGA CCCCAAATAA
     1201 TTGTGGAGCT CTGAATTTTA CAAGCTATTT TAATTACAAG GGAGAGCAGT GCCATACCCT
     1261 GAACAGCGGA GCTAAAGGAC ATGAACTGCT GAAGGTGAGC GATGTGGGAA TCTCTCATGG
     1321 ATACGAACTG GTGTTTGACC TGCAGACAAA CGAGGTTATT AAGAACTATC AGCTGAGCGG
     1381 AATGAGAATC GTGATCCATG ATCAGGTGTT CCCACCACAG CTGGTGGATG GATTCTTTAT
     1441 CAGCCCAGGA TTCAAGACCT ACATCAAGCT GGGAATTACC CAGTCTCAGT CTCTGCCACC
     1501 ACCATATTCT ACAGAATGCG GACAGAAAAA ACTGAAGTAT TATGCTATCT ATAGCCAGAG
     1561 ACTGTGCCTG CTGGAGACCC TGACAGACTT CACAGGAGAC CTGTGCGGAT GTAGAGATGT
     1621 GTTTATGCCA GAGAACGGAC TGCCATTCTG TAGCCTGCAG GAACTGTATA GCTGCATGTA
     1681 CCCAGCTAAA GAGTCTTTTA GCGAATTCAC CATGAGAAAA GAGTGTCCAA GCGATTGCGA
     1741 AGAAAGAACC TTTAGCTACG AGCTGTCTGA GGCTAGATAT CTGCACAATC CACCAATTGG
     1801 ACTGTCTCTG AGCAGACTGG ATAAACTGCA GCTGGACAAG TCTCTGCCAA GCGAGGCTCA
     1861 CCTGTCTAAA CTGGCTAAAA GCCTGACACC AAAAGAACTG GATGCCTACA TCGAAAGCAA
     1921 CATCATCAGC GTGATTCTGT TTTTCGGAGA TACCAGAATC GATTACCACG AACAGGAAGC
     1981 CACAAACGAC TTTTTCCAGT TCCTGGGAAA TATGGGAGGA GAATTTGGAC TGATGCTGGG
     2041 AGCTTCCCTG CTGACCTTCG TGGAATTCAT TGACCTGTTT ATTGTGCTGC TGTACCACCA
     2101 GATGCTGAGA CTGTATAACC TGAGAAAGAT TCCAGACATT TTTGCCAGCA AAAGAAACAG
     2161 AAATAAGGAA AAAACCAAGA AGTGTGAGAA CGTGGGTGGC TCAGGTGGTG GCGGTTCAGG
     2221 CGGAATGgga tccatgGTCT CAAAGGGTGA AGAAGATAAC ATGGCAATTA TTAAAGAGTT
     2281 TATGCGTTTC AAGGTGCATA TGGAGGGATC TGTCAATGGG CATGAGTTTG AAATTGAAGG
     2341 TGAAGGAGAA GGCCGACCAT ATGAGGGAAC ACAAACCGCA AAACTAAAGG TAAGTTTAAA
     2401 CATATATATA CTAACTAACC CTGATTATTT AAATTTTCAG GTAACTAAAG GCGGACCATT
     2461 ACCATTCGCC TGGGACATCC TCTCTCCACA GTTCATGTAT GGAAGTAAAG CTTATGTTAA
     2521 ACATCCGGCA GATATACCAG ATTATTTGAA ACTTTCATTC CCGGAGGGTT TTAAGTGGGA
     2581 ACGCGTAATG AATTTTGAAG ACGGAGGAGT TGTTACAGTG ACGCAAGACT CAAGGTAAGT
     2641 TTAAACAGTT CGGTACTAAC TAACCATACA TATTTAAATT TTCAGCCTCC AAGATGGAGA
     2701 ATTTATTTAT AAAGTCAAAC TTCGAGGAAC GAATTTCCCC TCGGATGGAC CTGTTATGCA
     2761 GAAGAAGACT ATGGGATGGG AAGCTTCAAG TGAAAGAATG TACCCTGAAG ACGGTGCTCT
     2821 TAAGGGAGAG ATTAAACAAC GTCTTAAATT GAAAGATGGA GGACATTACG ATGCTGAGGT
     2881 AAGTTTAAAC ATGATTTTAC TAACTAACTA ATCTGATTTA AATTTTCAGG TGAAGACAAC
     2941 TTACAAAGCC AAAAAACCAG TTCAGCTGCC AGGAGCGTAC AATGTTAATA TTAAACTGGA
     3001 TATCACCTCC CACAACGAGG ATTACACTAT CGTTGAGCAA TATGAAAGAG CTGAAGGGCG
     3061 GCACTCGACA GGTGGCATGG ATGAATTGTA TAAGtagCTT GTACAAAGTT GGCATTATAA
     3121 GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA
     3181 TTATTTGCCA TCCAGCTGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT
     3241 CCTGGCAGCT CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAATAA
     3301 TATCATCATG AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA
     3361 GCCATATTCA ACGGGAAACG TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT
     3421 ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT
     3481 ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG
     3541 ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA
     3601 TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA
     3661 AAACAGCATT CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC
     3721 TGGCAGTGTT CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG
     3781 ATCGCGTATT TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA
     3841 GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA
     3901 AACTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC
     3961 TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG
     4021 ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC
     4081 AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC
     4141 ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA
     4201 GCATTACGCT GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT AACGTGAGTT
     4261 ACGCGTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC
     4321 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG
     4381 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG
     4441 CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT
     4501 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
     4561 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC
     4621 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG
     4681 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG
     4741 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG
     4801 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
     4861 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT
     4921 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTT 
//
