LOCUS       pJH202_pE12_hyCa        4654 bp ds-DNA     circular     10-SEP-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Insert from 710520-5 15 to 1423
COMMENT     Backbone from pJH19_pDONR221 2897 to 651
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(2915..2935)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="forestgreen"
                     /ApEinfo_revcolor="dodgerblue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="forestgreen"
                     /ApEinfo_revcolor="dodgerblue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2270..2785
                     /locus_tag="Sequenced"
                     /label="Sequenced"
                     /ApEinfo_label="Sequenced"
                     /ApEinfo_fwdcolor="#ff6fcf"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     570..651
                     /locus_tag="AttP1"
                     /label="AttP1"
                     /ApEinfo_label="AttP1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     570..651
                     /locus_tag="AttL1"
                     /label="AttL1"
                     /ApEinfo_label="AttL1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2072..2785
                     /locus_tag="egfp"
                     /label="egfp"
                     /ApEinfo_label="egfp"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2895..2916)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="forestgreen"
                     /ApEinfo_revcolor="dodgerblue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3048..3854
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2789..2802)
                     /locus_tag="AttB2"
                     /label="AttB2"
                     /ApEinfo_label="AttB2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(2789..2877)
                     /locus_tag="AttP2"
                     /label="AttP2"
                     /ApEinfo_label="AttP2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(2789..2877)
                     /locus_tag="AttL2"
                     /label="AttL2"
                     /ApEinfo_label="AttL2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(2789..2802)
                     /locus_tag="AttR2"
                     /label="AttR2"
                     /ApEinfo_label="AttR2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    645..651
                     /locus_tag="AttB1"
                     /label="AttB1"
                     /ApEinfo_label="AttB1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2789..2795)
                     /locus_tag="Gateway Recomb2"
                     /label="Gateway Recomb2"
                     /ApEinfo_label="Gateway Recomb2"
                     /ApEinfo_fwdcolor="red"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     645..651
                     /locus_tag="AttR1"
                     /label="AttR1"
                     /ApEinfo_label="AttR1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2765..2785
                     /locus_tag="RPGW egfp"
                     /label="RPGW egfp"
                     /ApEinfo_label="RPGW egfp"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             683..2041
                     /locus_tag="hyNaC9"
                     /label="hyNaC9"
                     /ApEinfo_label="hyNaC9"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2765..2785
                     /locus_tag="RP egfp GW"
                     /label="RP egfp GW"
                     /ApEinfo_label="RP egfp GW"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    2042..2071
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2072..2090
                     /locus_tag="FP egfp GW"
                     /label="FP egfp GW"
                     /ApEinfo_label="FP egfp GW"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTA GCTAGCGCCA CCATGAGCGA GGAAGATGAG AAGAAAATTA AGAGCCCACA
      721 TGAACAGAGA AATGATAGAG TGAAAGAACA CGTGGCTCAT CTGATTAAGA ATGTGAGCTT
      781 CCATGGACTG AGCTATGTGG CTGATAAAAG AAACAACTAC TTCAGAAGAG CTATTTGGTT
      841 CCTGATCACC GTGGGAGCCT TCATCTATGC TGTGGAAAAG GTGTATGAGT CTACCGTGAA
      901 TTACTTTAGC TATCCATTCA AGACAGCTAG AATGAAAATC TATGTGAATG AACTGAACTT
      961 CCCAGCTGTG AGCTTCTGCA ATCTGAACGA TTTTCTGTTT TCTAAGCTGA ATGGAACCAA
     1021 ACTGGATGAA AGCATCCTGT ACCCAGATGA TCCAGAGAAA AACAATGTGT CTGAAATTGA
     1081 GATTTCCAAT ATCACATCTG ACGCTACAAT CAGACTGGAT CAGATGCTGG TGGATTGCGA
     1141 ATTCGAGGGA AAAAAGTGTA CACACGAGAA TTTCACCGAT TTCTGGACCA TGCAGGGAGA
     1201 GCTGTGCTTC ACATTCAATA GCGGAAAAAA TTCTCACTCT CTGCTGAAGG TGTCTGGAGT
     1261 GGGAGTGCTG AGATCTCTGA AGCTGACCAT TAACGTGCAG CATTATGAGT ATTACAGAGA
     1321 TGAGATGGCC GGAGGAATTC ACCTGATGAT TCATGGACAG GATGAAGAGC CAGTGAAAAT
     1381 GCAGGGACAG ATCGTGTCTC CAGGATATTC TTTCTATGTG AAGGTGGAAA AGAAGACCAT
     1441 CATGAACCTG GAGAAACCAT ATAAGACAGA GTGCGGAACC GTGAAACTGA AATATTTTGA
     1501 TAGATACTCC ATGCACACAT GCTGGCTGGA GCAGCTGACC GATTATGTGA ATAAGATGTG
     1561 CCACTGCAAG GACTTCTTCA TGCCAGGAAA TATTCCATAT TGTTCTCTGC CAGAACTGCA
     1621 GAACTGTACC TGGATTGAGT GGGCTAAATT CAATAAGGAT AAAATGTACA AGTGCCCACT
     1681 GCCATGTAAG ATTGACCTGT ACGGAGTGTC TCTGTCTAGA GCCCTGTTCC CAACCACACA
     1741 GTATAGCTCT ATCCTGGCCG AGCAGTTCAG AAAACAGCCA CATGTGCTGT CTATCGTGCA
     1801 CAACATTACA GATGAGCTGC TGTTCATGAG AGATAACCTG CTGAGATTCA TTATCTATTA
     1861 CGATGATCTG AGCTATGAGG TGCTGGAACA GAAACCATCT TATGAGACAC TGGTGTGGCT
     1921 GGGAGACATT GGAGGACAGA TCGGACTGTT TATCGGAGCC GGAGTGATGA GCTACTTCGA
     1981 GTTCCTGGAT TGCCTGGCCA TCGTGATCTA CACCAGATTT TTCCAGAAGT TCACAAGCAG
     2041 CGGTGGCTCA GGTGGTGGCG GTTCAGGCGG Aatggtgagc aagggcgagg agctgttcac
     2101 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     2161 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     2221 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     2281 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2341 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2401 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2461 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     2521 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     2581 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2641 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     2701 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2761 cactctcggc atggacgagc tgtactagCT TGTACAAAGT TGGCATTATA AGAAAGCATT
     2821 GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGCC
     2881 ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT CATAGCTGTT TCCTGGCAGC
     2941 TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAATA ATATCATCAT
     3001 GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC
     3061 AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA
     3121 AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC
     3181 CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG
     3241 ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT
     3301 TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT
     3361 TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT
     3421 TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT
     3481 TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG
     3541 ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC
     3601 CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG
     3661 ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC
     3721 AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC
     3781 TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC
     3841 TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC
     3901 TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT TAACGTGAGT TACGCGTCGT
     3961 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC
     4021 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC
     4081 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC
     4141 CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
     4201 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT
     4261 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT
     4321 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT
     4381 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT
     4441 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG
     4501 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT
     4561 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
     4621 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT  
//
