LOCUS       pJH203_pE12_hyCa        4810 bp ds-DNA     circular     10-SEP-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Insert from 710520-5 15 to 1423
COMMENT     Backbone from pJH19_pDONR221 2897 to 651
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(3071..3091)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="forestgreen"
                     /ApEinfo_revcolor="dodgerblue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="forestgreen"
                     /ApEinfo_revcolor="dodgerblue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     570..651
                     /locus_tag="AttP1"
                     /label="AttP1"
                     /ApEinfo_label="AttP1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     570..651
                     /locus_tag="AttL1"
                     /label="AttL1"
                     /ApEinfo_label="AttL1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3051..3072)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="forestgreen"
                     /ApEinfo_revcolor="dodgerblue"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3204..4010
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2945..2958)
                     /locus_tag="AttB2"
                     /label="AttB2"
                     /ApEinfo_label="AttB2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(2945..3033)
                     /locus_tag="AttP2"
                     /label="AttP2"
                     /ApEinfo_label="AttP2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(2945..3033)
                     /locus_tag="AttL2"
                     /label="AttL2"
                     /ApEinfo_label="AttL2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(2945..2958)
                     /locus_tag="AttR2"
                     /label="AttR2"
                     /ApEinfo_label="AttR2"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(2084..2236,2288..2481,2533..2725,2777..2941)
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#339933"
                     /ApEinfo_revcolor="#339933"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    645..651
                     /locus_tag="AttB1"
                     /label="AttB1"
                     /ApEinfo_label="AttB1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2945..2951)
                     /locus_tag="Gateway Recomb2"
                     /label="Gateway Recomb2"
                     /ApEinfo_label="Gateway Recomb2"
                     /ApEinfo_fwdcolor="red"
                     /ApEinfo_revcolor="red"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     645..651
                     /locus_tag="AttR1"
                     /label="AttR1"
                     /ApEinfo_label="AttR1"
                     /ApEinfo_fwdcolor="orange"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            2072..2080
                     /locus_tag="mCherry(1)"
                     /label="mCherry(1)"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             683..2041
                     /locus_tag="hyNaC9"
                     /label="hyNaC9"
                     /ApEinfo_label="hyNaC9"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2042..2071
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTA GCTAGCGCCA CCATGAGCGA GGAAGATGAG AAGAAAATTA AGAGCCCACA
      721 TGAACAGAGA AATGATAGAG TGAAAGAACA CGTGGCTCAT CTGATTAAGA ATGTGAGCTT
      781 CCATGGACTG AGCTATGTGG CTGATAAAAG AAACAACTAC TTCAGAAGAG CTATTTGGTT
      841 CCTGATCACC GTGGGAGCCT TCATCTATGC TGTGGAAAAG GTGTATGAGT CTACCGTGAA
      901 TTACTTTAGC TATCCATTCA AGACAGCTAG AATGAAAATC TATGTGAATG AACTGAACTT
      961 CCCAGCTGTG AGCTTCTGCA ATCTGAACGA TTTTCTGTTT TCTAAGCTGA ATGGAACCAA
     1021 ACTGGATGAA AGCATCCTGT ACCCAGATGA TCCAGAGAAA AACAATGTGT CTGAAATTGA
     1081 GATTTCCAAT ATCACATCTG ACGCTACAAT CAGACTGGAT CAGATGCTGG TGGATTGCGA
     1141 ATTCGAGGGA AAAAAGTGTA CACACGAGAA TTTCACCGAT TTCTGGACCA TGCAGGGAGA
     1201 GCTGTGCTTC ACATTCAATA GCGGAAAAAA TTCTCACTCT CTGCTGAAGG TGTCTGGAGT
     1261 GGGAGTGCTG AGATCTCTGA AGCTGACCAT TAACGTGCAG CATTATGAGT ATTACAGAGA
     1321 TGAGATGGCC GGAGGAATTC ACCTGATGAT TCATGGACAG GATGAAGAGC CAGTGAAAAT
     1381 GCAGGGACAG ATCGTGTCTC CAGGATATTC TTTCTATGTG AAGGTGGAAA AGAAGACCAT
     1441 CATGAACCTG GAGAAACCAT ATAAGACAGA GTGCGGAACC GTGAAACTGA AATATTTTGA
     1501 TAGATACTCC ATGCACACAT GCTGGCTGGA GCAGCTGACC GATTATGTGA ATAAGATGTG
     1561 CCACTGCAAG GACTTCTTCA TGCCAGGAAA TATTCCATAT TGTTCTCTGC CAGAACTGCA
     1621 GAACTGTACC TGGATTGAGT GGGCTAAATT CAATAAGGAT AAAATGTACA AGTGCCCACT
     1681 GCCATGTAAG ATTGACCTGT ACGGAGTGTC TCTGTCTAGA GCCCTGTTCC CAACCACACA
     1741 GTATAGCTCT ATCCTGGCCG AGCAGTTCAG AAAACAGCCA CATGTGCTGT CTATCGTGCA
     1801 CAACATTACA GATGAGCTGC TGTTCATGAG AGATAACCTG CTGAGATTCA TTATCTATTA
     1861 CGATGATCTG AGCTATGAGG TGCTGGAACA GAAACCATCT TATGAGACAC TGGTGTGGCT
     1921 GGGAGACATT GGAGGACAGA TCGGACTGTT TATCGGAGCC GGAGTGATGA GCTACTTCGA
     1981 GTTCCTGGAT TGCCTGGCCA TCGTGATCTA CACCAGATTT TTCCAGAAGT TCACAAGCAG
     2041 CGGTGGCTCA GGTGGTGGCG GTTCAGGCGG AATGggatcc atgGTCTCAA AGGGTGAAGA
     2101 AGATAACATG GCAATTATTA AAGAGTTTAT GCGTTTCAAG GTGCATATGG AGGGATCTGT
     2161 CAATGGGCAT GAGTTTGAAA TTGAAGGTGA AGGAGAAGGC CGACCATATG AGGGAACACA
     2221 AACCGCAAAA CTAAAGGTAA GTTTAAACAT ATATATACTA ACTAACCCTG ATTATTTAAA
     2281 TTTTCAGGTA ACTAAAGGCG GACCATTACC ATTCGCCTGG GACATCCTCT CTCCACAGTT
     2341 CATGTATGGA AGTAAAGCTT ATGTTAAACA TCCGGCAGAT ATACCAGATT ATTTGAAACT
     2401 TTCATTCCCG GAGGGTTTTA AGTGGGAACG CGTAATGAAT TTTGAAGACG GAGGAGTTGT
     2461 TACAGTGACG CAAGACTCAA GGTAAGTTTA AACAGTTCGG TACTAACTAA CCATACATAT
     2521 TTAAATTTTC AGCCTCCAAG ATGGAGAATT TATTTATAAA GTCAAACTTC GAGGAACGAA
     2581 TTTCCCCTCG GATGGACCTG TTATGCAGAA GAAGACTATG GGATGGGAAG CTTCAAGTGA
     2641 AAGAATGTAC CCTGAAGACG GTGCTCTTAA GGGAGAGATT AAACAACGTC TTAAATTGAA
     2701 AGATGGAGGA CATTACGATG CTGAGGTAAG TTTAAACATG ATTTTACTAA CTAACTAATC
     2761 TGATTTAAAT TTTCAGGTGA AGACAACTTA CAAAGCCAAA AAACCAGTTC AGCTGCCAGG
     2821 AGCGTACAAT GTTAATATTA AACTGGATAT CACCTCCCAC AACGAGGATT ACACTATCGT
     2881 TGAGCAATAT GAAAGAGCTG AAGGGCGGCA CTCGACAGGT GGCATGGATG AATTGTATAA
     2941 GtagCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC
     3001 AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG
     3061 AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT
     3121 GATGTTACAT TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA
     3181 TAAACAGTAA TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT
     3241 TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC
     3301 AATCAGGTGC GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA
     3361 AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC
     3421 TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT
     3481 GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG
     3541 ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC
     3601 CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC
     3661 GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG
     3721 TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA
     3781 CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA
     3841 TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT
     3901 GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA
     3961 ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT
     4021 TGGTTAATTG GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT
     4081 CATGACCAAA ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG
     4141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     4201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     4261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     4321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     4381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     4441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     4501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     4561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     4621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     4681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     4741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     4801 CTCACATGTT     
//
