LOCUS       pAAV-CAMKII-iGECI1.3.3        6125 bp    DNA     circular SYN 22-OCT-2018
DEFINITION  AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes) .
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CW3SL-EGFP.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Choi JH, Yu NK, Baek GC, Bakes J, Seo D, Nam HJ, Baek SH, Lim CS, 
            Lee YS, Kaang BK
  TITLE     Optimization of AAV expression cassettes to improve packaging 
            capacity and transgene expression in neurons.
  JOURNAL   Mol Brain. 2014 Mar 11;7:17. doi: 10.1186/1756-6606-7-17.
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 10, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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FEATURES             Location/Qualifiers
     repeat_region   1..141
                     /vntifkey="34"
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus serotype 2"
     repeat_region   complement(3388..3528)
                     /vntifkey="34"
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus serotype 2"
     rep_origin      3603..4058
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=f1\ori
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     CDS             4445..5305
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     rep_origin      5476..6064
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=pBR322\origin
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     misc_feature    563..568
                     /vntifkey="21"
                     /label=Kozak\sequence
     promoter        150..543
                     /vntifkey="29"
                     /label=CAMKII\promoter
     misc_feature    1475..1918
                     /vntifkey="21"
                     /label=CaM
     misc_feature    1943..2020
                     /vntifkey="21"
                     /label=M13
     misc_feature    2027..2938
                     /vntifkey="21"
                     /label=miRFP720
     misc_feature    569..1468
                     /vntifkey="21"
                     /label=miRFP670(-13N)
BASE COUNT     1348 a      1664 c      1678 g      1435 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca gatgctgcac acttgtggac taagtttgtt 
      181 cgcatcccct tctccaaccc cctcagtaca tcaccctggg ggaacagggt ccacttgctc 
      241 ctgggcccac acagtcctgc agtattgtgt atataaggcc agggcaaaga ggagcaggtt 
      301 ttaaagtgaa aggcaggcag gtgttgggga ggcagttacc ggggcaacgg gaacagggcg 
      361 tttcggaggt ggttgccatg gggacctgga tgctgacgaa ggctcgcgag gctgtgagca 
      421 gccacagtgc cctgctcaga agccccaagc tcgtcagtca agccggttct ccgtttgcac 
      481 tcaggagcac gggcaggcga gtggccccta gttctggggg cagcgtgaaa agacggtatc 
      541 gataagcttg attcgagcta gcgccaccat ggcctctcat tcgaattgcg aacatgaaga 
      601 gatccacctc gccggctcga tccagccgca tggcgcgctt ctggtcgtca gcgaacatga 
      661 tcatcgcgtc atccaggcca gcgccaacgc cgcggaattt ctgaatctcg gaagcgtact 
      721 cggcgttccg ctcgccgaga tcgacggcga tctgttgatc aagatcctgc cgcatctcga 
      781 tcccaccgcc gaaggcatgc cggtcgcggt gcgctgccgg atcggcaatc cctctacgga 
      841 gtactgcggt ctgatgcatc ggcctccgga aggcgggctg atcatcgaac tcgaacgtgc 
      901 cggcccgtcg atcgatctgt caggcacgct ggcgccggcg ctggagcgga tccgcacggc 
      961 gggttcactg cgcgcgctgt gcgatgacac cgtgctgctg tttcagcagt gcaccggcta 
     1021 cgaccgggtg atggtgtatc gtttcgatga gcaaggccac ggcctggtat tctccgagtg 
     1081 ccatgtgcct gggctcgaat cctatttcgg caaccgctat ccgtcgtcga ctgtcccgca 
     1141 gatggcgcgg cagctgtacg tgcggcagcg cgtccgcgtg ctggtcgacg tcacctatca 
     1201 gccggtgccg ctggagccgc ggctgtcgcc gctgaccggg cgcgatctcg acatgtcggg 
     1261 ctgcttcctg cgctcgatgt cgccgtgcca tctgcagttc ctgaaggaca tgggcgtgcg 
     1321 cgccaccctg gcggtgtcgc tggtggtcgg cggcaagctg tggggcctgg ttgtctgtca 
     1381 ccattatctg ccgcgcttca tccgtttcga gctgcgggcg atctgcaaac ggctcgccga 
     1441 aaggatcgcg acgcggatca ccgcgcttta cacccatgac caactgacag aagagcagat 
     1501 tgcagagttc aaagaagcct tctcattatt cgacaaggat ggggacggca ccatcaccac 
     1561 aaaggaactt ggcaccgtta tgaggtcgct tggacaaaac ccaacggaag cagaattgca 
     1621 ggatatgatc aatgaagtcg atgctgatgg caatggaacg atttactttc ctgaatttct 
     1681 tactatgatg gctagaaaaa tgaaggacac agacagcgaa gaggaaatcc gagaagcatt 
     1741 ccgtgttttt gacaaggatg ggaacggcta catcagcgct gctgaattac gtcacgtcat 
     1801 gacaaacctc ggggagaagt taacagatga agaagttgat gaaatgataa gggaagcaga 
     1861 tatcgatggt gatggccaag taaactatga agagtttgta caaatgatga cagcaaaggg 
     1921 ggggggtggc ggaggtggct ccaagaggcg ctggaagaaa aacttcattg ccgtcagcgc 
     1981 tgccaaccgg ttcaagaaga tatccagctc cggggcactg gtagtaacct gcgacgatga 
     2041 gccgatccat atccccggtg ccatccaacc gcatggactg ctgctcgccc tcgccgccga 
     2101 catgacgatc gttgccggca gcgacaacct tcccgaactc accggactgg cgatcggcgc 
     2161 cctgatcggc cgctctgcgg ccgatgtctt cgactcggag acgcacaacc gtctgacgat 
     2221 cgccttggcc gagcccgggg cggccgtcgg agcaccgatc actgtcggct tcacgatgcg 
     2281 aaaggacgca ggcttcatcg gctcctggca tcgccatgat cagctcatct tcctcgagct 
     2341 cgagcctccc cagcgggacg tcgccgagcc gcaggcgttc ttccgccgca ccaacagcgc 
     2401 catccgccgc ctgcaggccg ccgaaacctt ggaaagcgcc tgcgccgccg cggcgcaaga 
     2461 ggtgcggaag attaccggct tcgatcgggt gatgatctat cgcttcgcct ccgacttcag 
     2521 cgggtccgtg atcgcagagg atcggtgcgc cgaggtcgag tcaaaactag gcctgcacta 
     2581 tcctgcctca ttcatcccgg cgcaggcccg tcggctctat accatcaacc cggtacggat 
     2641 cattcccgat atcaattatc ggccggtgcc ggtcacccca gacctcaatc cggtcaccgg 
     2701 gcggccgatt gatcttagct tcgccatcct gcgcagcgtc tcgcccaacc atctggagtt 
     2761 catgcgcaac ataggcatgc acggcacgat gtcgatctcg attttgcgcg gcgagcgact 
     2821 gtggggattg atcgtttgcc atcaccgaac gccgtactac gtcgatctcg atggccgcca 
     2881 agcctgcaag aggctcgccg agaggctggc cactcagatc ggcgtgatgg aagagtgaga 
     2941 attccgctcg agataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 
     3001 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 
     3061 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg 
     3121 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 
     3181 gcactgacaa ttccgtggtg tttatttgtg aaatttgtga tgctattgct ttatttgtaa 
     3241 ccatctagct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc 
     3301 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag 
     3361 atgtgggagg ttttttaaag cggccgcagg aacccctagt gatggagttg gccactccct 
     3421 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct 
     3481 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc 
     3541 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata 
     3601 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 
     3661 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 
     3721 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 
     3781 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg 
     3841 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 
     3901 tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt 
     3961 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 
     4021 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat 
     4081 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc 
     4141 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 
     4201 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt 
     4261 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg 
     4321 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 
     4381 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 
     4441 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 
     4501 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 
     4561 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 
     4621 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 
     4681 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 
     4741 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 
     4801 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 
     4861 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 
     4921 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 
     4981 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 
     5041 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 
     5101 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 
     5161 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 
     5221 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 
     5281 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 
     5341 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 
     5401 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 
     5461 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 
     5521 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 
     5581 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 
     5641 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 
     5701 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 
     5761 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 
     5821 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 
     5881 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 
     5941 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 
     6001 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 
     6061 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 
     6121 catgt 
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