LOCUS       pAAV-CAMKII-iGECI1.3.3        6194 bp    DNA     circular SYN 22-OCT-2018
DEFINITION  AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes) .
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CW3SL-EGFP.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Choi JH, Yu NK, Baek GC, Bakes J, Seo D, Nam HJ, Baek SH, Lim CS, 
            Lee YS, Kaang BK
  TITLE     Optimization of AAV expression cassettes to improve packaging 
            capacity and transgene expression in neurons.
  JOURNAL   Mol Brain. 2014 Mar 11;7:17. doi: 10.1186/1756-6606-7-17.
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 10, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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FEATURES             Location/Qualifiers
     repeat_region   12..141
                     /vntifkey="34"
                     /label=AAV2\ITR
     primer_bind     complement(3077..3097)
                     /vntifkey="28"
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   complement(3457..3586)
                     /vntifkey="34"
                     /label=AAV2\ITR
     CDS             4514..5374
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        150..543
                     /vntifkey="29"
                     /label=CAMKII\promoter
     misc_feature    569..1492
                     /vntifkey="21"
                     /label=emiRFP670
     misc_feature    1499..1942
                     /vntifkey="21"
                     /label=CaM
     misc_feature    1967..2044
                     /vntifkey="21"
                     /label=M13
     misc_feature    2051..2962
                     /vntifkey="21"
                     /label=miRFP720
     misc_feature    2960..3007
                     /vntifkey="21"
                     /label=NES
BASE COUNT     1358 a      1689 c      1702 g      1445 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca gatgctgcac acttgtggac taagtttgtt 
      181 cgcatcccct tctccaaccc cctcagtaca tcaccctggg ggaacagggt ccacttgctc 
      241 ctgggcccac acagtcctgc agtattgtgt atataaggcc agggcaaaga ggagcaggtt 
      301 ttaaagtgaa aggcaggcag gtgttgggga ggcagttacc ggggcaacgg gaacagggcg 
      361 tttcggaggt ggttgccatg gggacctgga tgctgacgaa ggctcgcgag gctgtgagca 
      421 gccacagtgc cctgctcaga agccccaagc tcgtcagtca agccggttct ccgtttgcac 
      481 tcaggagcac gggcaggcga gtggccccta gttctggggg cagcgtgaaa agacggtatc 
      541 gataagcttg attcgagcta gcgccaccat ggcggaaggc tccgtcgcca ggcagcctga 
      601 cctcttgacc tgcgaacatg aagagatcca cctcgccggc tcgatccagc cgcatggcgc 
      661 gcttctggtc gtcagcgaac atgatcatcg cgtcatccag gccagcgcca acgccgcgga 
      721 atttctgaat ctcggaagcg tactcggcgt tccgctcgcc gagatcgacg gcgatctgtt 
      781 gatcaagatc ctgccgcatc tcgatcccac cgccgaaggc atgccggtcg cggtgcgctg 
      841 ccggatcggc aatccctcta cggagtactg cggtctgatg catcggcctc cggaaggcgg 
      901 gctgatcatc gaactcgaac gtgccggccc gtcgatcgat ctgtcaggca cgctggcgcc 
      961 ggcgctggag cggatccgca cggcgggttc actgcgcgcg ctgtgcgatg acaccgtgct 
     1021 gctgtttcag cagtgcaccg gctacgaccg ggtgatggtg tatcgtttcg atgagcaagg 
     1081 ccacggcctg gtattctccg agtgccatgt gcctgggctc gaatcctatt tcggcaaccg 
     1141 ctatccgtcg tcgactgtcc cgcagatggc gcggcagctg tacgtgcggc agcgcgtccg 
     1201 cgtgctggtc gacgtcacct atcagccggt gccgctggag ccgcggctgt cgccgctgac 
     1261 cgggcgcgat ctcgacatgt cgggctgctt cctgcgctcg atgtcgccgt gccatctgca 
     1321 gttcctgaag gacatgggcg tgcgcgccac cctggcggtg tcgctggtgg tcggcggcaa 
     1381 gctgtggggc ctggttgtct gtcaccatta tctgccgcgc ttcatccgtt tcgagctgcg 
     1441 ggcgatctgc aaacggctcg ccgaaaggat cgcgacgcgg atcaccgcgc tttacaccca 
     1501 tgaccaactg acagaagagc agattgcaga gttcaaagaa gccttctcat tattcgacaa 
     1561 ggatggggac ggcaccatca ccacaaagga acttggcacc gttatgaggt cgcttggaca 
     1621 aaacccaacg gaagcagaat tgcaggatat gatcaatgaa gtcgatgctg atggcaatgg 
     1681 aacgatttac tttcctgaat ttcttactat gatggctaga aaaatgaagg acacagacag 
     1741 cgaagaggaa atccgagaag cattccgtgt ttttgacaag gatgggaacg gctacatcag 
     1801 cgctgctgaa ttacgtcacg tcatgacaaa cctcggggag aagttaacag atgaagaagt 
     1861 tgatgaaatg ataagggaag cagatatcga tggtgatggc caagtaaact atgaagagtt 
     1921 tgtacaaatg atgacagcaa aggggggggg tggcggaggt ggctccaaga ggcgctggaa 
     1981 gaaaaacttc attgccgtca gcgctgccaa ccggttcaag aagatatcca gctccggggc 
     2041 actggtagta acctgcgacg atgagccgat ccatatcccc ggtgccatcc aaccgcatgg 
     2101 actgctgctc gccctcgccg ccgacatgac gatcgttgcc ggcagcgaca accttcccga 
     2161 actcaccgga ctggcgatcg gcgccctgat cggccgctct gcggccgatg tcttcgactc 
     2221 ggagacgcac aaccgtctga cgatcgcctt ggccgagccc ggggcggccg tcggagcacc 
     2281 gatcactgtc ggcttcacga tgcgaaagga cgcaggcttc atcggctcct ggcatcgcca 
     2341 tgatcagctc atcttcctcg agctcgagcc tccccagcgg gacgtcgccg agccgcaggc 
     2401 gttcttccgc cgcaccaaca gcgccatccg ccgcctgcag gccgccgaaa ccttggaaag 
     2461 cgcctgcgcc gccgcggcgc aagaggtgcg gaagattacc ggcttcgatc gggtgatgat 
     2521 ctatcgcttc gcctccgact tcagcgggtc cgtgatcgca gaggatcggt gcgccgaggt 
     2581 cgagtcaaaa ctaggcctgc actatcctgc ctcattcatc ccggcgcagg cccgtcggct 
     2641 ctataccatc aacccggtac ggatcattcc cgatatcaat tatcggccgg tgccggtcac 
     2701 cccagacctc aatccggtca ccgggcggcc gattgatctt agcttcgcca tcctgcgcag 
     2761 cgtctcgccc aaccatctgg agttcatgcg caacataggc atgcacggca cgatgtcgat 
     2821 ctcgattttg cgcggcgagc gactgtgggg attgatcgtt tgccatcacc gaacgccgta 
     2881 ctacgtcgat ctcgatggcc gccaagcctg caagaggctc gccgagaggc tggccactca 
     2941 gatcggcgtg atggaagagg gaggtggaac cctgcagctg cctcctctgg aacgcctgac 
     3001 tctggatgaa ttccgctcga gataatcaac ctctggatta caaaatttgt gaaagattga 
     3061 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 
     3121 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 
     3181 tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 
     3241 ggctgttggg cactgacaat tccgtggtgt ttatttgtga aatttgtgat gctattgctt 
     3301 tatttgtaac catctagctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc 
     3361 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt 
     3421 cagggggaga tgtgggaggt tttttaaagc ggccgcagga acccctagtg atggagttgg 
     3481 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac 
     3541 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc 
     3601 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa 
     3661 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
     3721 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
     3781 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
     3841 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc 
     3901 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
     3961 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc 
     4021 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
     4081 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct 
     4141 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc 
     4201 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt 
     4261 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa 
     4321 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac 
     4381 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 
     4441 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 
     4501 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 
     4561 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 
     4621 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 
     4681 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 
     4741 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 
     4801 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 
     4861 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 
     4921 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 
     4981 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 
     5041 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 
     5101 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 
     5161 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 
     5221 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 
     5281 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 
     5341 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 
     5401 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 
     5461 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 
     5521 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 
     5581 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 
     5641 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 
     5701 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 
     5761 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 
     5821 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 
     5881 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 
     5941 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 
     6001 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 
     6061 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 
     6121 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 
     6181 ttttgctcac atgt 
//
