LOCUS       Exported                9133 bp ds-DNA     circular SYN 13-APR-2020
DEFINITION  Cloning vector pUC57, complete sequence.
ACCESSION   Y14837
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Markausakas A, Dreguniene G.
  TITLE     A new cloning vector pUC57
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Markauskas A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8, 
            Vilnius 2028, LITHUANIA
REFERENCE   3  (bases 1 to 9133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 11, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     http://www.informaxinc.com/ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    402..987
                     /label=P element 5' end
     protein_bind    988..1035
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    1036..1105
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    1118..1217
                     /label=Mhc Splice Acceptor
     misc_feature    1216..1257
                     /label=Phase 2 front linker
     CDS             1258..2751
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     regulatory      2770..2779
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2776..3483
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    3484..3526
                     /label=Phase 2 linker back
     misc_feature    3525..3645
                     /label=Mhc Splice Donor
     protein_bind    3658..3727
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             join(3728..3799,4194..4467,4542..5196,5258..5573,5794..5925,
                     5996..6610)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    6611..6658
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    6659..6891
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(6912..6928)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6936..6952
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6960..6990)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7005..7026
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7314..7902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8073..8933)
                     /codon_start=1
                     /label=AmpR
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8934..9038)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accagatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtactag tcatgatgaa ataacataag
      421 gtggtcccgt cgatagccga agcttaccga agtatacact taaattcagt gcacgtttgc
      481 ttgttgagag gaaaggttgt gtgcggacga attttttttt gaaaacatta acccttacgt
      541 ggaataaaaa aaaatgaaat attgcaaatt ttgctgcaaa gctgtgactg gagtaaaatt
      601 aattcacgtg ccgaagtgtg ctattaagag aaaattgtgg gagcagagcc ttgggtgcag
      661 ccttggtgaa aactcccaaa tttgtgatac ccactttaat gattcgcagt ggaaggctgc
      721 acctgcaaaa ggtcagacat ttaaaaggag gcgactcaac gcagatgccg tacctagtaa
      781 agtgatagag cctgaaccag aaaagataaa agaaggctat accagtggga gtacacaaac
      841 agagtaagtt tgaatagtaa aaaaaatcat ttatgtaaac aataacgtga ctgtgcgtta
      901 ggtcctgttc attgtttaat gaaaataaga gcttgaggga aaaaattcgt actttggagt
      961 acgaaatgcg tcgtttagag cagcagcgaa gttcctattc cgaagttcct attctctaga
     1021 aagtatagga acttcctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
     1081 gggcgcgtac tccacctcac ccatcgtcga ctctagaagt cgatccaaca tggcgacttg
     1141 tcccatcccc ggcatgttta aatatactaa ttattcttga actaatttta atcaaccgat
     1201 ttatctctct tccgcaggtc gggaggttcc ggtggaagcg gaggtagcgg cggatccatg
     1261 aagatggaca aaaagactat agtttggttt agaagagacc taaggattga ggataatcct
     1321 gcattagcag cagctgctca cgaaggatct gtttttcctg tcttcatttg gtgtcctgaa
     1381 gaagaaggac agttttatcc tggaagagct tcaagatggt ggatgaaaca atcacttgct
     1441 cacttatctc aatccttgaa ggctcttgga tctgacctca ctttaatcaa aacccacaac
     1501 acgatttcag cgatcttgga ttgtatccgc gttaccggtg ctacaaaagt cgtctttaac
     1561 cacctctatg atcctgtttc gttagttcgg gaccataccg taaaggagaa gctggtggaa
     1621 cgtgggatct ctgtgcaaag ctacaatgga gatctattgt atgaaccgtg ggagatatac
     1681 tgcgaaaagg gcaaaccttt tacgagtttc aattcttact ggaagaaatg cttagatatg
     1741 tcgattgaat ccgttatgct tcctcctcct tggcggttga tgccaataac tgcagcggct
     1801 gaagcgattt gggcgtgttc gattgaagaa ctagggctgg agaatgaggc cgagaaaccg
     1861 agcaatgcgt tgttaactag agcttggtct ccaggatgga gcaatgctga taagttacta
     1921 aatgagttca tcgagaagca gttgatagat tatgcaaaga acagcaagaa agttgttggg
     1981 aattctactt cactactttc tccgtatctc catttcgggg aaataagcgt cagacacgtt
     2041 ttccagtgtg cccggatgaa acaaattata tgggcaagag ataagaacag tgaaggagaa
     2101 gaaagtgcag atctttttct taggggaatc ggtttaagag agtattctcg gtatatatgt
     2161 ttcaacttcc cgtttactca cgagcaatcg ttgttgagtc atcttcggtt tttcccttgg
     2221 gatgctgatg ttgataagtt caaggcctgg agacaaggca ggaccggtta tccgttggtg
     2281 gatgccggaa tgagagagct ttgggctacc ggatggatgc ataacagaat aagagtgatt
     2341 gtttcaagct ttgctgtgaa gtttcttctc cttccatgga aatggggaat gaagtatttc
     2401 tgggatacac ttttggatgc tgatttggaa tgtgacatcc ttggctggca gtatatctct
     2461 gggagtatcc ccgatggcca cgagcttgat cgcttggaca atcccgcgtt acaaggcgcc
     2521 aaatatgacc cagaaggtga gtacataagg caatggcttc ccgagcttgc gagattgcca
     2581 actgaatgga tccatcatcc atgggacgct cctttaaccg tactcaaagc ttctggtgtg
     2641 gaactcggaa caaactatgc gaaacccatt gtagacatcg acacagctcg tgagctacta
     2701 gctaaagcta tttcaagaac ccgtgaagca cagatcatga tcggagcagc agcccgggat
     2761 ccaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag
     2821 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     2881 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     2941 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     3001 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     3061 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     3121 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     3181 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     3241 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     3301 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     3361 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     3421 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     3481 aagggcggat ccggaggtag cggtggaagc ggaggttccg gcaggtaagt tattgaacaa
     3541 tggcatcaaa tgccttcatc atcactaccc tttagccctt aagaccccac aatgacctta
     3601 cccactcaga gaaaaaagta aatatgaaag cccatttgaa cttctaagct tgtcgacgat
     3661 gggtgaggtg gagtacgcgc ccggggagcc caagggcacg ccctggcacc cgcaccgcgg
     3721 cttcgagatg ggccaagagg atcaggagct attaattcgc ggaggcagca aacacccatc
     3781 tgccgagcat ctgaacaatg tgagtagtac atgtgcatac atcttaagtt cacttgatct
     3841 ataggaactg cgattgcaac atcaaattgt ctgcggcgtg agaactgcga cccacaaaaa
     3901 tcccaaaccg caatcgcaca aacaaatagt gacacgaaac agattattct ggtagctgtg
     3961 ctcgctatat aagacaattt ttaagatcat atcatgatca agacatctaa aggcattcat
     4021 tttcgactac attctttttt acaaaaaata taacaaccag atattttaag ctgatcctag
     4081 atgcacaaaa aataaataaa agtataaacc tacttcgtag gatacttcgt tttgttcggg
     4141 gttagatgag cataacgctt gtagttgata tttgagatcc cctatcattg cagggtgaca
     4201 gcggagcggc ttcgcagagc tgcattaacc agggcttcgg gcaggccaaa aactacggca
     4261 cgctcctgcc acccagtccg ccggaggact ccggttcagg gagcggccaa ctagccgaga
     4321 acctcaccta tgcctggcac aatatggaca tctttggggc ggtcaatcag ccgggctccg
     4381 gatggcggca gctggtcaac cggacacgcg gactattctg caacgagcga cacataccgg
     4441 cgcccaggaa acatttgctc aagaacggtg agtttctatt cgcagtcggc tgatctgtgt
     4501 gaaatcttaa taaagggtcc aattaccaat ttgaaactca gtttgcggcg tggcctatcc
     4561 gggcgaactt ttggccgtga tgggcagttc cggtgccgga aagacgaccc tgctgaatgc
     4621 ccttgccttt cgatcgccgc agggcatcca agtatcgcca tccgggatgc gactgctcaa
     4681 tggccaacct gtggacgcca aggagatgca ggccaggtgc gcctatgtcc agcaggatga
     4741 cctctttatc ggctccctaa cggccaggga acacctgatt ttccaggcca tggtgcggat
     4801 gccacgacat ctgacctatc ggcagcgagt ggcccgcgtg gatcaggtga tccaggagct
     4861 ttcgctcagc aaatgtcagc acacgatcat cggtgtgccc ggcagggtga aaggtctgtc
     4921 cggcggagaa aggaagcgtc tggcattcgc ctccgaggca ctaaccgatc cgccgcttct
     4981 gatctgcgat gagcccacct ccggactgga ctcatttacc gcccacagcg tcgtccaggt
     5041 gctgaagaag ctgtcgcaga agggcaagac cgtcatcctg accattcatc agccgtcttc
     5101 cgagctgttt gagctctttg acaagatcct tctgatggcc gagggcaggg tagctttctt
     5161 gggcactccc agcgaagccg tcgacttctt ttcctagtga gttcgatgtg tttattaagg
     5221 gtatctagca ttacattaca tctcaactcc tatccagcgt gggtgcccag tgtcctacca
     5281 actacaatcc ggcggacttt tacgtacagg tgttggccgt tgtgcccgga cgggagatcg
     5341 agtcccgtga tcggatcgcc aagatatgcg acaattttgc tattagcaaa gtagcccggg
     5401 atatggagca gttgttggcc accaaaaatt tggagaagcc actggagcag ccggagaatg
     5461 ggtacaccta caaggccacc tggttcatgc agttccgggc ggtcctgtgg cgatcctggc
     5521 tgtcggtgct caaggaacca ctcctcgtaa aagtgcgact tattcagaca acggtgagtg
     5581 gttccagtgg aaacaaatga tataacgctt acaattcttg gaaacaaatt cgctagattt
     5641 tagttagaat tgcctgattc cacacccttc ttagtttttt tcaatgagat gtatagttta
     5701 tagttttgca gaaaataaat aaatttcatt taactcgcga acatgttgaa gatatgaata
     5761 ttaatgagat gcgagtaaca ttttaatttg cagatggttg ccatcttgat tggcctcatc
     5821 tttttgggcc aacaactcac gcaagtgggc gtgatgaata tcaacggagc catcttcctc
     5881 ttcctgacca acatgacctt tcaaaacgtc tttgccacga taaatgtaag tcttgtttag
     5941 aatacatttg catattaata atttactaac tttctaatga atcgattcga tttaggtgtt
     6001 cacctcagag ctgccagttt ttatgaggga ggcccgaagt cgactttatc gctgtgacac
     6061 atactttctg ggcaaaacga ttgccgaatt accgcttttt ctcacagtgc cactggtctt
     6121 cacggcgatt gcctatccga tgatcggact gcgggccgga gtgctgcact tcttcaactg
     6181 cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc ttcggatatc taatatcctg
     6241 cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg ccggttatca taccattcct
     6301 gctctttggc ggcttcttct tgaactcggg ctcggtgcca gtatacctca aatggttgtc
     6361 gtacctctca tggttccgtt acgccaacga gggtctgctg attaaccaat gggcggacgt
     6421 ggagccgggc gaaattagct gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt
     6481 catcctggag acgcttaact tctccgccgc cgatctgccg ctggactacg tgggtctggc
     6541 cattctcatc gtgagcttcc gggtgctcgc atatctggct ctaagacttc gggcccgacg
     6601 caaggagtag gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttctg
     6661 cgtactcgca aattattaaa aataaaactt taaaaataat ttcgtctaat taatattatg
     6721 agttaattca aaccccacgg acatgctaag ggttaatcaa caatcatatc gctgtctcac
     6781 tcagactcaa tacgacactc agaatactat tcctttcact cgcacttatt gcaagcatac
     6841 gttaagtgga tgtctcttgc cgacgggacc accttatgtt atttcatcat ggcggccgcg
     6901 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6961 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7021 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7081 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7141 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7201 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7261 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7321 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7381 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7441 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7501 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7561 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7621 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7681 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7741 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7801 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7861 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7921 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7981 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8041 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8101 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8161 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgactccca
     8221 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8281 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8341 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8401 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8461 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8521 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8581 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8641 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8701 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8761 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8821 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8881 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8941 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9001 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9061 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     9121 aggccctttc gtc
//
