LOCUS       pCDH_CAG_NCre_T2A_mTagBFP2        9107 bp    DNA     circular      5-SEP-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-11926800|
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FEATURES             Location/Qualifiers
     misc_feature    5176..5730
                     /vntifkey="21"
                     /label=NCreIntN
     misc_feature    5800..6516
                     /vntifkey="21"
                     /label=mTagBFP2
     misc_feature    5740..5793
                     /vntifkey="21"
                     /label=T2A
     misc_feature    3141..3160
                     /vntifkey="21"
                     /label=seqF1
     misc_feature    5228..5247
                     /vntifkey="21"
                     /label=seqF2
     misc_feature    5931..5950
                     /vntifkey="21"
                     /label=seqF3
     misc_feature    3308..5161
                     /vntifkey="21"
                     /label=CAG\promoter
BASE COUNT     2185 a      2270 c      2521 g      2131 t 
ORIGIN
        1 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
       61 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
      121 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
      181 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
      241 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
      301 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
      361 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
      421 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
      481 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
      541 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
      601 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
      661 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
      721 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
      781 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
      841 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
      901 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 
      961 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 
     1021 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 
     1081 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 
     1141 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 
     1201 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 
     1261 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 
     1321 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgac 
     1381 gcgtgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac 
     1441 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg 
     1501 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat 
     1561 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct 
     1621 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 
     1681 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 
     1741 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc 
     1801 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct 
     1861 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
     1921 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
     1981 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
     2041 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
     2101 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
     2161 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
     2221 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
     2281 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
     2341 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     2401 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     2461 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc 
     2521 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     2581 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     2641 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     2701 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     2761 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 
     2821 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 
     2881 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 
     2941 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 
     3001 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 
     3061 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag 
     3121 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt 
     3181 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca 
     3241 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt 
     3301 tatcgatgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     3361 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     3421 aggcgccgga attcgatgtc gacattgatt attgactagt tattaatagt aatcaattac 
     3481 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 
     3541 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 
     3601 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggactatt tacggtaaac 
     3661 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 
     3721 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 
     3781 ttggcagtac atctacgtat tagtcatcgc tattaccatg ggtcgaggtg agccccacgt 
     3841 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt 
     3901 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg 
     3961 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc 
     4021 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag 
     4081 cgaagcgcgc ggcgggcggg agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc 
     4141 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg 
     4201 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct cgtttctttt 
     4261 ctgtggctgc gtgaaagcct taaagggctc cgggagggcc ctttgtgcgg gggggagcgg 
     4321 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc 
     4381 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg 
     4441 ggagcgcggc cgggggcggt gccccgcggt gcgggggggc tgcgagggga acaaaggctg 
     4501 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta 
     4561 acccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc 
     4621 tccgtgcggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg 
     4681 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc 
     4741 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc 
     4801 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgg cggagccgaa atctgggagg 
     4861 cgccgccgca ccccctctag cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa 
     4921 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc 
     4981 ggggctgccg cagggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt 
     5041 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt 
     5101 cctacagctc ctgggcaacg tgctggttgt tgtgctgtct catcattttg gcaaagaatt 
     5161 cgcggccgcg ccaccatgcc caagaagaag cggaaggtgt acccatacga tgttccagat 
     5221 tacgcaggtt ctggttccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc 
     5281 gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg 
     5341 ttttctgagc atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc 
     5401 aagttgaatg aattccttaa ggggtgttta agctatgaaa cggaaatatt gacagtagaa 
     5461 tatggatcat taccgattgg taaaattgta gaaaagcgca tcgaatgtac tgtttatagt 
     5521 gttgataata atggaaatat ttatacacaa cctgtagcac aatggcacga tcgcggagaa 
     5581 caagaggtgt ttgagtattg tttggaagat ggttcattga ttcgggcaac aaaagaccat 
     5641 aagtttatga ctgttgatgg tcaaatgttg ccaattgatg aaatatttga acgtgaattg 
     5701 gatttgatgc gggttgataa tttgccgaat tatgctagcg agggcagagg aagtcttcta 
     5761 acatgcggtg acgtggagga gaatcccggc cctggatcca tggtgtctaa gggcgaagag 
     5821 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac 
     5881 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc 
     5941 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc 
     6001 tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc 
     6061 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc 
     6121 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg 
     6181 gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc 
     6241 accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag 
     6301 ctcgtgggcg ggagccatct gatcgcaaac gccaagacca catatagatc caagaaaccc 
     6361 gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag 
     6421 gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac 
     6481 ctccctagca aactggggca caagcttaat cactagggcg cgcccacctg gtgcatgacc 
     6541 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 
     6601 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 
     6661 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 
     6721 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 
     6781 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 
     6841 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 
     6901 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg 
     6961 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 
     7021 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 
     7081 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 
     7141 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 
     7201 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct 
     7261 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 
     7321 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 
     7381 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 
     7441 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 
     7501 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 
     7561 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 
     7621 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact 
     7681 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 
     7741 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 
     7801 tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg 
     7861 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 
     7921 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 
     7981 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 
     8041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     8101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     8161 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     8221 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     8281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     8341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     8401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 
     8461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     8521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     8581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     8641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     8701 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     8761 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     8821 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     8881 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     8941 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     9001 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     9061 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagat 
//
