LOCUS       pCDH_CAG_CCre_T2A_CopGFP        9663 bp    DNA     circular      4-SEP-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|-11925621|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     misc_feature    3141..3160
                     /vntifkey="21"
                     /label=seqF1
     misc_feature    5176..6255
                     /vntifkey="21"
                     /label=IntC-CCre
     misc_feature    6262..6315
                     /vntifkey="21"
                     /label=T2A
     misc_feature    6322..7080
                     /vntifkey="21"
                     /label=CopGFP
     misc_feature    5170..5175
                     /vntifkey="21"
                     /label=kozak
     misc_feature    3308..5161
                     /vntifkey="21"
                     /label=CAG\promoter
BASE COUNT     2271 a      2473 c      2691 g      2228 t 
ORIGIN
        1 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
       61 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
      121 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
      181 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
      241 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
      301 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
      361 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
      421 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
      481 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
      541 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
      601 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
      661 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
      721 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
      781 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
      841 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
      901 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 
      961 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 
     1021 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 
     1081 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 
     1141 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 
     1201 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 
     1261 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 
     1321 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgac 
     1381 gcgtgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac 
     1441 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg 
     1501 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat 
     1561 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct 
     1621 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 
     1681 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 
     1741 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc 
     1801 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct 
     1861 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 
     1921 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 
     1981 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 
     2041 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 
     2101 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 
     2161 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 
     2221 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 
     2281 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 
     2341 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 
     2401 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 
     2461 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc 
     2521 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 
     2581 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 
     2641 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 
     2701 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 
     2761 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 
     2821 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 
     2881 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 
     2941 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 
     3001 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 
     3061 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag 
     3121 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt 
     3181 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca 
     3241 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt 
     3301 tatcgatgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     3361 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     3421 aggcgccgga attcgatgtc gacattgatt attgactagt tattaatagt aatcaattac 
     3481 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 
     3541 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 
     3601 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggactatt tacggtaaac 
     3661 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 
     3721 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 
     3781 ttggcagtac atctacgtat tagtcatcgc tattaccatg ggtcgaggtg agccccacgt 
     3841 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt 
     3901 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg 
     3961 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc 
     4021 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag 
     4081 cgaagcgcgc ggcgggcggg agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc 
     4141 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg 
     4201 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct cgtttctttt 
     4261 ctgtggctgc gtgaaagcct taaagggctc cgggagggcc ctttgtgcgg gggggagcgg 
     4321 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc 
     4381 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg 
     4441 ggagcgcggc cgggggcggt gccccgcggt gcgggggggc tgcgagggga acaaaggctg 
     4501 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta 
     4561 acccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc 
     4621 tccgtgcggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg 
     4681 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc 
     4741 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc 
     4801 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgg cggagccgaa atctgggagg 
     4861 cgccgccgca ccccctctag cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa 
     4921 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc 
     4981 ggggctgccg cagggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt 
     5041 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt 
     5101 cctacagctc ctgggcaacg tgctggttgt tgtgctgtct catcattttg gcaaagaatt 
     5161 cttaattaag ccaccatgga ttataaagac gatgacgata aaatggttaa agttatcggt 
     5221 cgtcgttccc tcggagtgca aagaatattt gatattggtc ttcgccaaga ccataatttt 
     5281 ctgctagcca atggggcgat cgcggccaat tgcttcaata aggaggacgg atccaaccgg 
     5341 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc 
     5401 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg 
     5461 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatacga 
     5521 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat 
     5581 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg 
     5641 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg 
     5701 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa 
     5761 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc 
     5821 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg 
     5881 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg 
     5941 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag 
     6001 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc 
     6061 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc 
     6121 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat 
     6181 ggcgatgaat tctgcagata tccatcacac tggcggccgc tcgagatgga ctacaaggac 
     6241 gacgatgata agggcaccgg tgagggcaga ggaagtcttc taacatgcgg tgacgtggag 
     6301 gagaatcccg gccctcccgg gatggagagc gacgagagcg gcctgcccgc catggagatc 
     6361 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag 
     6421 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc 
     6481 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac 
     6541 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc 
     6601 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag 
     6661 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg 
     6721 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc 
     6781 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac 
     6841 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag 
     6901 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg 
     6961 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct 
     7021 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccct gtacaagtaa 
     7081 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg 
     7141 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 
     7201 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 
     7261 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 
     7321 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 
     7381 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 
     7441 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 
     7501 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg 
     7561 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 
     7621 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat 
     7681 ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg 
     7741 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 
     7801 gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 
     7861 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 
     7921 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 
     7981 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 
     8041 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 
     8101 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 
     8161 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 
     8221 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     8281 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 
     8341 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc 
     8401 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 
     8461 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 
     8521 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 
     8581 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 
     8641 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 
     8701 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 
     8761 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 
     8821 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 
     8881 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 
     8941 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 
     9001 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 
     9061 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 
     9121 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 
     9181 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 
     9241 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 
     9301 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 
     9361 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 
     9421 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 
     9481 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 
     9541 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 
     9601 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 
     9661 gat 
//
