LOCUS       pGS101_pNH605-Gal-5UTR- 8765 bp ds-DNA     circular     03-SEP-2020
DEFINITION  .
KEYWORDS    "creator:Lindquist Lab" "marker:5'UTR, AmpR"                       
FEATURES             Location/Qualifiers
     promoter        complement(5313..5331)
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     8698..8714
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     CDS             6762..7553
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_difference 4031..4033
                     /label="MCS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        276..683
                     /label="LEU2 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      5495..5950
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     5'UTR           2988..3004
                     /label="5'UTR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    8674..8690
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     primer_bind     complement(5338..5354)
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     CDS             6693..6761
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2950..2973
                     /label="GAL1_primer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        8642..8659
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      7724..8312
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        6588..6692
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             3005..3130
                     /label="signal sequence Kar2"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        8636..8641
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        2537..2987
                     /label="GAL1_promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2497..2515
                     /label="MCS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4048..4135
                     /label="ADH1 terminator from C.albicans"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             3131..4030
                     /label="hApoE4"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     promoter        8660..8666
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        8735..8753
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    8600..8621
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccGAG GAGAACTTCT AGTATATCTA
       61 CATACCTAAT ATTATTGCCT TATTAAAAAT GGAATCCCAA CAATTACATC AAAATCCACA
      121 TTCTCTTCAA AATCAATTGT CCTGTACTTC CTTGTTCATG TGTGTTCAAA AACGTTATAT
      181 TTATAGGATA ATTATACTCT ATTTCTCAAC AAGTAATTGG TTGTTTGGCC GAGCGGTCTA
      241 AGGCGCCTGA TTCAAGAAAT ATCTTGACCG CAGTTAACTG TGGGAATACT CAGGTATCGT
      301 AAGATGCAAG AGTTCGAATC TCTTAGCAAC CATTATTTTT TTCCTCAACA TAACGAGAAC
      361 ACACAGGGGC GCTATCGCAC AGAATCAAAT TCGATGACTG GAAATTTTTT GTTAATTTCA
      421 GAGGTCGCCT GACGCATATA CCTTTTTCAA CTGAAAAATT GGGAGAAAAA GGAAAGGTGA
      481 GAGCGCCGGA ACCGGCTTTT CATATAGAAT AGAGAAGCGT TCATGACTAA ATGCTTGCAT
      541 CACAATACTT GAAGTTGACA ATATTATTTA AGGACCTATT GTTTTTTCCA ATAGGTGGTT
      601 AGCAATCGTC TTACTTTCTA ACTTTTCTTA CCTTTTACAT TTCAGCAATA TATATATATA
      661 TATTTCAAGG ATATACCATT CTAATGTCTG CCCCTAAGTT ACCAATAGAT AGCGGTTTAA
      721 TATAAGGTTT CAACAAGAAA TATTTTGTGA AAATCAAAAG TTAATAGTTT CACTGTCGAA
      781 AATATGAAAG TTTAAGTTTT CAGTTCATCC CATTGTAGTT ATCAAATTTC TGTGTTTCCC
      841 GTATATGTGT GCTCTGTATT CATCGCTATC TTGAAACTGG TTATGATCGT TGCGCATGTG
      901 ACCTGTATCG CTCAAAAGAG CCCGGCACCG GTACCGGGCA AAAGCCCCTA GCAAGCTAAA
      961 ATGATTCAAC TTTCGTAGTA ATTTGTTCAA CCGGTCACAG TAAAGTCAAT GACTAACTTT
     1021 TGAATCGAGG ATGTATTTCC AATTGAAAGC AATGTATATA TAAACCATTG TGTTGAAGCT
     1081 TGTCTTACAG TTTTCTTGTA TCCTCCCATT GTTCATATAG TACTTAAAGT ACCATAATCC
     1141 TTAAAGAACA AACTTGAAAG AGAATACACT ATACACAACT AATAAATAAA GAATAATCAT
     1201 GGCTGTGACC AAGACAATTG TAGTTCTACC AGGTGACCAT GTTGGTCAAG AAATCACTGA
     1261 AGAGGCCATT AAAGTTTTAA ATGCTATTCA GGAATGTCGT CCAGACAAGG TCAATTTCAA
     1321 GTTTGATCAT CATTTGATCG GTGGTGCTGC AATTGATGCC ACTGGTGTTC CATTACCAGA
     1381 CGAAGCTTTG GAGGCCTCTA AGAAAGCTGA TGCCGTGCTT CTGGGTGCTG TTGGTGGTCC
     1441 AAAATGGGGT ACTGGCGCTG TCAGACCAGA ACAAGGTCTT TTGAAAATCC GTAAGGAGTT
     1501 GCAATTGTAT GCTAATCTAA GACCATGTAA TTTTGCATCT GATTCCTTAC TAGATCTATC
     1561 GCCATTGAAG CCTGAAATTG CAAGAGGTAC AGATTTCGTC GTTGTTAGAG AACTAGTGGG
     1621 TGGTATTTAT TTCGGGGAGA GAAAAGAAGA TGAAGGTGAT GGTGTCGCCT GGGATAGCGA
     1681 AAAGTATTCT GTGCCCGAAG TTCAAAGAAT CACTAGAATG GCTGCTTTCA TGGCTCTGCA
     1741 ACACAACCCA CCTTTACCTA TTTGGTCTCT AGATAAAGCC AACGTTCTAG CATCATCAAG
     1801 ATTATGGAGA AAAACAGTGG AAGAGACGAT TAAGAATGAA TTCCCACAAT TGACTGTTCA
     1861 ACATCAATTG ATTGATTCCG CTGCTATGAT CTTGGTTAAG AACCCAACTC ATCTAAATGG
     1921 TATTATTATC ACAAACAATA TGTTTGGTGA TATTATCTCC GATGAAGCCT CCGTGATCCC
     1981 AGGTTCGTTA GGATTACTTC CATCTGCCTC CTTGGCATCT CTTCCAGATA AGAATACGGC
     2041 ATTTGGTTTA TATGAGCCAT GTCATGGCTC TGCTCCAGAC TTACCAAAGG GAAAGGTTAA
     2101 CCCCGTAGCA ACAATCCTAT CTGCTGCTAT GATGTTGAAG TTGTCTCTGG ACCTGTTCGA
     2161 AGAAGGTGAA ATTATAGAAC AAGCAGTCAA GAAAGTGTTG GATTCTGGTA TCAGAACAGC
     2221 GGATCTAAAG GGTACCAACT CTACTACTGA GGTCGGTGAT GCAGTAGCGA AAGCTGTCAG
     2281 GGAACTATTA GCTTAGACTT AGTAGATGTA CAGTTGCATA CATAAGTCTG AACAGAAAGA
     2341 AGTAAATATC ATATCTAGTT CGGTTTAGAA ATCAGTGACT ATATTGGTTT TGACCAGATC
     2401 GCAATCCTTG CAAGCATTTA GTAATGACTA TCAAACCGGT GAATCATCTG ATAAGGCTAT
     2461 ATTAGCTTGT GCATTCGCAT GTATCGGGAA ACGAACGGTA CCGGGCCCaa atacCaacaa
     2521 aagctggagc tctagtacgg attagaagcc gccgagcggg Tgacagccct ccgaAggaag
     2581 actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg
     2641 cgccgcactg ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg
     2701 aaaaattggc agtaacctgg ccccacaaac cttcaaatGa acgaatcaaa ttaacaacca
     2761 taggatgata atgcgattag ttttttagcc ttatttctgg ggtaattaat cagcgaagcg
     2821 atgatttttg atctattaac agatatataa atgCaaaaAc tgcataacca ctttaactaa
     2881 tactttcaac attttcGgtt tgtattactt cttattcaaa tgtAataaaa gtatcaacaa
     2941 aaaattgtta atatacctct atactttaac gtcaaggaga aaaaaccaaa acaactaatt
     3001 cgaaATGTTT TTCAACAGAC TAAGCGCTGG CAAGCTGCTG GTACCACTCT CCGTGGTCCT
     3061 GTACGCCCTT TTCGTGGTAA TATTACCTTT ACAGAATTCT TTCCACTCCT CCAATGTTTT
     3121 AGTTAGAGGa aaggtggagc aagcggtgga gacagagccg gagcccgagc tgcgccagca
     3181 gaccgagtgg cagagcggcc agcgctggga actggcactg ggtcgctttt gggattacct
     3241 gcgctgggtg cagacactgt ctgagcaggt gcaggaggag ctgctcagct cccaggtcac
     3301 ccaggaactg agggcgctga tggacgagac catgaaggag ttgaaggcct acaaatcgga
     3361 actggaggaa caactgaccc cggtggcgga ggagacgcgg gcacggctgt ccaaggagct
     3421 gcaggcggcg caggcccggc tgggcgcgga catggaggac gtgcGCggcc gcctggtgca
     3481 gtaccgcggc gaggtgcagg ccatgctcgg ccagagcacc gaggagctgc gggtgcgcct
     3541 cgcctcccac ctgcgcaagc tgcgtaagcg gctcctccgc gatgccgatg acctgcagaa
     3601 gcgcctggca gtgtaccagg ccggggcccg cgagggcgcc gagcgcggcc tcagcgccat
     3661 ccgcgagcgc ctggggcccc tggtggaaca gggccgcgtg cgggccgcca ctgtgggctc
     3721 cctggccggc cagccgctac aggagcgggc ccaggcctgg ggcgagcggc tgcgcgcgcg
     3781 gatggaggag atgggcagcc ggacccgcga ccgcctggac gaggtgaagg agcaggtggc
     3841 ggaggtgcgc gccaagctgg aggagcaggc ccagcagata cgcctgcagg ccgaggcctt
     3901 ccaggcccgc ctcaagagct ggttcgagcc cctggtggaa gacatgcagc gccagtgggc
     3961 cgggctggtg gagaaggtgc aggctgccgt gggcaccagc gccgcccctg tgcccagcga
     4021 caatcactga ccaccgcggt ggagctcTAA GCAAATAGCT AAATTATATA CGAATTAATA
     4081 TTATGATTAA GTGTTTACGT GAGTGCGATA TTTTTATTAC TATCTTATAC AGTTGTATAT
     4141 ACTCTATAAA ATGAGTTGTC TATTAATTAA CGCGATGAAT GCTTTCTGGG TTTACCTCTC
     4201 CAACAACTCT AGTTTACTTC TCAATACATT CAATTGTATT TGATTTGTCA ATACTTCATC
     4261 ATTAATCAAT TCTATAGTTT TGTTTTTCTC GTTTATTTCC AAATTTAATG CATCAATTTT
     4321 ATTATTCAAT TTGTCGTTGA TTTTGGTTAA TGATTTTATG GTTTGATCTC TGGCATTGAT
     4381 TGTTTGTGTT AGTTTTTCAT TATTGATAat taaaTTATTT AAGTTAGTTA TCAACTCGGT
     4441 GTTTTCAAGT TTCAAGTTTT CAATTTCTTT AGAGTTTATT AGATTTGTCA AAGTTTCTGA
     4501 ATTGCTTGAT TGGTCCTGTA GAAGAGTATT TGTTGTTGTG GATAATTGAT TCAATTTTTG
     4561 AGACAATTGC TGGAAGGCGT TGAAATATCT AGCATCAATC TCATGGTTTT TTTCCCGAGA
     4621 GTCTCGTAGA TTCAATTGTT TTAATATATC TTGGGACCAC TCTTGATTTG AACTCATGGA
     4681 AattaaaCTG GGTGTTGTGT TGTGGTGTAA TGATTGTACC CCCTTTGCTT ATAATTGTGT
     4741 GGCCACCGAA GTCGGTGATG CTGTCGCCGA AGAAGTTAAG AAAATCCTTG CTTAAAAAGA
     4801 TTCTCTTTTT TTATGATATT TGTACATAAA CTTTATAAAT GAAATTCATA ATAGAAACGA
     4861 CACGAAATTA CAAAATGGAA TATGTTCATA GGGTAGACGA AACTATATAC GCAATCTACA
     4921 TACATTTATC AAGAAGGAGA AAAAGGAGGA TGTAAAGGAA TACAGGTAAG CAAATTGATA
     4981 CTAATGGCTC AACGTGATAA GGAAAAAGAA TTGCACTTTA ACATTAATAT TGACAAGGAG
     5041 GAGGGCACCA CACAAAAAGT TAGGTGTAAC AGAAAATCAT GAAACTATGA TTCCTAATTT
     5101 ATATATTGGA GGATTTTCTC TAAAAAAAAA AAAATACAAC AAATAAAAAA CACTCAATGA
     5161 CCTGACCATT TGATGGAGTT TAAGTCAATA CCTTCTTGAA CCATTTCCCA TAATGGTGAA
     5221 AGTTCCCTCA AGAATTTTAC TCTGTCAGAA ACGGCCTTAA CGACGTAggc cggccattta
     5281 aatgtttaaa cggccacgct ggccCAATTC GCCCTATAGT GAGTCGTATT ACAATTCACT
     5341 GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT
     5401 TGCAGCACAT CCCCCtTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC
     5461 TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGACGCGC CCTGTAGCGG CGCATTAAGC
     5521 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC
     5581 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT
     5641 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA
     5701 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC
     5761 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA
     5821 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT
     5881 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG
     5941 cTTACAATTT CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCa
     6001 tAGGGTAATA actgatataa ttaaattgaa gctctaattt gtgagtttag tattaggatc
     6061 tctacccttg gcgaaaagtc ctctgccaac aatgatgata tctgatccac cacttacaac
     6121 ttcgtcgacg gttctgtact gctgacccaa tatgcgtata tataccaatc taagtctgtg
     6181 ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaggaaa
     6241 ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aaaaggtggt ATGGTGCACT
     6301 CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC
     6361 GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC
     6421 GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA
     6481 AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAg
     6541 acgtcggcgc gccGAGGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA
     6601 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT
     6661 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC
     6721 TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA
     6781 AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA
     6841 ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT
     6901 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG
     6961 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC
     7021 ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA
     7081 ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT
     7141 TgCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG
     7201 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA
     7261 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG
     7321 AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG
     7381 CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG
     7441 ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG gAGTCAGGCA ACTATGGATG
     7501 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG
     7561 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA
     7621 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT
     7681 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC
     7741 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC
     7801 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC
     7861 CAAATACTGT tCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
     7921 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT
     7981 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT
     8041 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT
     8101 ACCTACAGCG TGAGCtaTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT
     8161 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGaAACG
     8221 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT
     8281 GATGCTCGTC AGGGGGGCgG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
     8341 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG
     8401 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG
     8461 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC
     8521 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG
     8581 GCAGTGAGCG CAACGCAATT AATGTGAGTT AgCTCACTCA TTAGGCACCC CAGGCTTTAC
     8641 ACTTTATGCT TCCGGCTCCT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG
     8701 GAAACAGCTA TGACCATGAT TACGCCAAGC TCGGAATTAA CCCTCACTAA AGGGAACAAA
     8761 AGCTG
//