LOCUS       GB2242_(pVD1_tRNA-gRNA[ 3229 bp ds-DNA     circular     30-NOV-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(1087..1090)
                     /label="OH [n-1]"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1089..1164)
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1090..1108
                     /label="PBS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1098..1101)
                     /label="OH [5-6]"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1187..1262)
                     /label="pre-tRNA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1245..1266)
                     /label="PBS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(1270..1273)
                     /label="OH [5]"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(join(2566..3229,1..194))
                     /label="AmpR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
       61 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
      121 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
      181 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
      241 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
      301 acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag
      361 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
      421 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
      481 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
      541 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
      601 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
      661 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
      721 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
      781 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc
      841 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
      901 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
      961 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
     1021 tgggcccgac gtcgcatgct cccggccgcc atggcggccg cgggaattcg attcccgtct
     1081 cgctcaTTGC ACCGACTCGG TGCCACTTTT TCAAGTTGAT AACGGACTAG CCTTATTTTA
     1141 ACTTGCTATT TCTAGCTCTA AAACtgagac ggcgcgcgcc gtctctGCAC CAGCCGGGAA
     1201 TCGAACCCGG GTCTGTACCG TGGCAGGGTA CTATTCTACC ACTAGACCAC TGGTGCTTTG
     1261 TTGCACCGAC TCGcgagcga gacgggatca ctagtgaatt cgcggccgcc tgcaggtcga
     1321 ccatatggga gagctcccaa cgcgttggat gcatagcttg agtattctat agtgtcacct
     1381 aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     1441 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     1501 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1561 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1621 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1681 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1741 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1801 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1861 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1921 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1981 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2041 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2101 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2161 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2221 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2281 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2341 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2401 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2461 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2521 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2581 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2641 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2701 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2761 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2821 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2881 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2941 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3001 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3061 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3121 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3181 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcatt
//