LOCUS       Exported                7695 bp ds-DNA     circular SYN 01-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Gouaux Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 25, 2020 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             687..1547
                     /codon_start=1
                     /label=Amp (R)
                     /note="vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1692..2365
                     /direction=RIGHT
                     /label=pUC origin
                     /note="vntifkey=33"
     primer_bind     2383..2402
                     /label=Tn7RF
     misc_recomb     2611..2835
                     /label=Tn7R
                     /note="vntifkey=86"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /label=Gm(R)
                     /note="vntifkey=4"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     3073..3092
                     /label=GM F
     polyA_site      4000..4281
                     /label=HSV TK polyA
                     /note="vntifkey=26"
     primer_bind     4000..4020
                     /label=HSV TK R
     misc_feature    4282..4332
                     /label=MCS II
                     /note="vntifkey=21"
     promoter        complement(4346..4468)
                     /label=P10 promoter
                     /note="P10
                     vntifkey=29"
     primer_bind     4506..4527
                     /label=pFBastBacF
     promoter        4507..4635
                     /label=PH promoter
                     /note="vntifkey=29"
     promoter        4641..5437
                     /label=CMV promoter
                     /note="vntifkey=29"
     primer_bind     4845..4868
                     /label=pCMV F1
     intron          5497..5629
                     /note="vntifkey=15"
     primer_bind     5508..5530
                     /label=pCMVF2
     misc_feature    5669..5689
                     /label=MCSI
                     /note="vntifkey=21"
     exon            5698..5721
                     /label=8_his_tag
     exon            5731..6444
                     /label=eGFP
     primer_bind     complement(6040..6061)
                     /label=4eGFPR
     primer_bind     6040..6061
                     /label=4eGFPF
     misc_feature    6463..6486
                     /label=3c protease site
     misc_feature    6488..6542
                     /label=MCSI
                     /note="vntifkey=21"
     misc_feature    6564..7155
                     /label=WPRE
                     /note="vntifkey=21"
     primer_bind     complement(6656..6676)
                     /label=WPRE R
     polyA_site      7179..7419
                     /label=SV40 pA
                     /note="vntifkey=26"
     primer_bind     complement(7251..7271)
                     /label=pFastbacR
     misc_recomb     7448..7613
                     /label=Tn7L
                     /note="vntifkey=86"
     primer_bind     complement(7672..7692)
                     /label=Tn7L R
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cagctttgtt taaacaaagc tggctatggc agggcttgcc
     4021 gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttgggggttg gggtggggaa
     4081 aaggaagaaa cgcgggcgta ttggtcccaa tggggtctcg gtggggtatc gacagagtgc
     4141 cagccctggg accgaacccc gcgtttatga acaaacgacc caacacccgt gcgttttatt
     4201 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     4261 agttagcctc ccccatctcc cggtaccgca tgctatgcat cagctgctag caccatggct
     4321 cgagatcccg ggtgatcaag tcttcgtcga gtgattgtaa ataaaatgta atttacagta
     4381 tagtatttta attaatatac aaatgatttg ataataattc ttatttaact ataatatatt
     4441 gtgttgggtt gaattaaagg tccgtatact agtatcgatt cgcgacctac tccggaatat
     4501 taatagatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact
     4561 gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc
     4621 ccaccatcgg gcgcggatcc tcaatattgg ccattagcca tattattcat tggttatata
     4681 gcataaatca atattggcta ttggccattg catacgttgt atctatatca taatatgtac
     4741 atttatattg gctcatgtcc aatatgaccg ccatgttggc attgattatt gactagttat
     4801 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     4861 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     4921 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     4981 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg
     5041 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     5101 ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     5161 atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca
     5221 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     5281 ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg
     5341 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcactagaag ctttattgcg
     5401 gtagtttatc acagttaaat tgctaacgca gtcagtgctt ctgacacaac agtctcgaac
     5461 ttaagctgca gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag
     5521 gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga
     5581 taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc
     5641 cagttcaatt acagctctta agggatcccg gtccgaagcg cgcggaatta caccatgcac
     5701 catcatcatc accaccatca cggttcagga gtgagcaagg gcgaggagct gttcaccggg
     5761 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     5821 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     5881 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     5941 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     6001 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     6061 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     6121 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     6181 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     6241 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     6301 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
     6361 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     6421 ctcggcatgg acgagctgta caagtccgga ctcagatctg gactggaagt tctgttccag
     6481 ggtcctgaat tcaaaggcct acgtcgacga gctcactagt cgcggccgct ttcgaatcta
     6541 gagcctgcag tctcgacaag cttaatcaac ctctggatta caaaatttgt gaaagattga
     6601 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6661 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6721 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6781 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6841 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6901 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     6961 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7021 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7081 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7141 ccgcctcccc gcctgaagct tgtcgagaag tactagagga tcataatcag ccataccaca
     7201 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     7261 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     7321 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7381 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct tgagcctagg
     7441 agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt
     7501 attagcttac gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc
     7561 ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc acagcggggc
     7621 atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg acaaaccgtc
     7681 atcttcggct acttt
//
