LOCUS       Exported                7690 bp ds-DNA     circular SYN 01-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Gouaux Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 25, 2020 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             687..1547
                     /codon_start=1
                     /label=Amp (R)
                     /note="vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1692..2365
                     /direction=RIGHT
                     /label=pUC origin
                     /note="vntifkey=33"
     primer_bind     2383..2402
                     /label=Tn7RF
     misc_recomb     2611..2835
                     /label=Tn7R
                     /note="vntifkey=86"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /label=Gm(R)
                     /note="vntifkey=4"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     3073..3092
                     /label=GM F
     polyA_site      4000..4281
                     /label=HSV TK polyA
                     /note="vntifkey=26"
     primer_bind     4000..4020
                     /label=HSV TK R
     misc_feature    4282..4332
                     /label=MCS II
                     /note="vntifkey=21"
     promoter        complement(4346..4468)
                     /label=P10 promoter
                     /note="P10
                     vntifkey=29"
     primer_bind     4506..4527
                     /label=pFBastBacF
     promoter        4507..4635
                     /label=PH promoter
                     /note="vntifkey=29"
     promoter        4641..5437
                     /label=CMV promoter
                     /note="vntifkey=29"
     primer_bind     4845..4868
                     /label=pCMV F1
     intron          5497..5629
                     /note="vntifkey=15"
     primer_bind     5508..5530
                     /label=pCMVF2
     misc_feature    5669..5739
                     /label=MCSI
                     /note="vntifkey=21"
     misc_feature    5741..5764
                     /label=3c protease site
     misc_feature    5777..6496
                     /label=GFP
                     /note="GFP"
     primer_bind     complement(6086..6107)
                     /label=4eGFPR
     primer_bind     6086..6107
                     /label=4eGFPF
     misc_feature    6506..6529
                     /label=His8 tag
     misc_feature    6533..6537
                     /label=MCSI
                     /note="vntifkey=21"
     misc_feature    6559..7150
                     /label=WPRE
                     /note="vntifkey=21"
     primer_bind     complement(6651..6671)
                     /label=WPRE R
     polyA_site      7174..7414
                     /label=SV40 pA
                     /note="vntifkey=26"
     primer_bind     complement(7246..7266)
                     /label=pFastbacR
     misc_recomb     7443..7608
                     /label=Tn7L
                     /note="vntifkey=86"
     primer_bind     complement(7667..7687)
                     /label=Tn7L R
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cagctttgtt taaacaaagc tggctatggc agggcttgcc
     4021 gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttgggggttg gggtggggaa
     4081 aaggaagaaa cgcgggcgta ttggtcccaa tggggtctcg gtggggtatc gacagagtgc
     4141 cagccctggg accgaacccc gcgtttatga acaaacgacc caacacccgt gcgttttatt
     4201 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     4261 agttagcctc ccccatctcc cggtaccgca tgctatgcat cagctgctag caccatggct
     4321 cgagatcccg ggtgatcaag tcttcgtcga gtgattgtaa ataaaatgta atttacagta
     4381 tagtatttta attaatatac aaatgatttg ataataattc ttatttaact ataatatatt
     4441 gtgttgggtt gaattaaagg tccgtatact agtatcgatt cgcgacctac tccggaatat
     4501 taatagatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact
     4561 gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc
     4621 ccaccatcgg gcgcggatcc tcaatattgg ccattagcca tattattcat tggttatata
     4681 gcataaatca atattggcta ttggccattg catacgttgt atctatatca taatatgtac
     4741 atttatattg gctcatgtcc aatatgaccg ccatgttggc attgattatt gactagttat
     4801 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     4861 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     4921 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     4981 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg
     5041 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     5101 ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     5161 atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca
     5221 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     5281 ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg
     5341 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcactagaag ctttattgcg
     5401 gtagtttatc acagttaaat tgctaacgca gtcagtgctt ctgacacaac agtctcgaac
     5461 ttaagctgca gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag
     5521 gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga
     5581 taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc
     5641 cagttcaatt acagctctta agggatcccg gtccgaagcg cgcggaattc aaaggcctac
     5701 gtcgacgagc tcactagtcg cggccgcttt cgaatctaga ctggaagttc tgttccaggg
     5761 tcctgcagcc gccgcggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     5821 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     5881 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     5941 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     6001 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     6061 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     6121 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     6181 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     6241 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     6301 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     6361 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
     6421 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     6481 gctgtacaag tccggaggtt caggacacca tcatcatcac caccatcact gactagagcc
     6541 tgcagtctcg acaagcttaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6601 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6661 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6721 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6781 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6841 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6901 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     6961 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7021 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7081 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7141 tccccgcctg aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt
     7201 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     7261 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     7321 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7381 caaactcatc aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctaggagatc
     7441 cgaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag
     7501 cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa
     7561 aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt
     7621 tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt
     7681 cggctacttt
//
