LOCUS       pTrichoGate-3           4048 bp    DNA     circular SYN 15-SEP-2020
DEFINITION  MN007107 Cloning vector pTrichoGate-3, complete sequence.
ACCESSION   MN007107
VERSION     MN007107.1
KEYWORDS    .
SOURCE      Cloning vector pTrichoGate-3
  ORGANISM  Cloning vector pTrichoGate-3
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Nogueira-Lopez,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd,
            Lincoln, Christchurch, Canterbury 7647, New Zealand
COMMENT     ##Imported_From-START##
            Path     :: C:\Users\mendozaa\Geneious 2020.1 Data\temp\66
            Filename :: Clipboard Contents 19
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Cloning vector pTrichoGate-3"
                     /mol_type="other DNA"
                     /db_xref="taxon:2604823"
                     /lab_host="Escherichia coli TOP10F'"
     gene            132..992
                     /gene="AmpR"
     CDS             132..992
                     /gene="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="ampicillin resistance"
                     /protein_id="QEG99019.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      2215..3238
                     /regulatory_class="promoter"
                     /note="translation elongation factor EF-1 alpha promoter
                     (Tef1P)"
ORIGIN      
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtggttc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagct tgcatgcctg caggtcgact ctagaggatc cccggtctct acaagtacca
     2221 gtacatcgtc cgagttgaaa cccaaacgaa cagcgttgag ttgggcaaaa actggcgcgc
     2281 gccagcagcc gagtgtgggt ggattgggca gtgaaaaaaa aaaaaaaaaa aaaagagaga
     2341 gacagagaga gtcagcagga gaagggtagc agcaggctaa tggttcctca ttttgggatt
     2401 tcgtcgtacc cattgatagc ttcctttgag catcagagcg tagcccacgt cagctgtttt
     2461 tctttgtctc ttctttcgcc gtctgtttgt gtcataccga gccgaacttg aggctcttgt
     2521 gtgatatcat ggacagcggc cgatgactct ttggggtttg cctaaaagac gatccgcaga
     2581 ggtaacgaat actacaggca ctcgtactat gtactaccta ctactcaggt gctactaaag
     2641 tagatggttt agcacgtact gggtacgcag gatgatcaaa gcggtgggag tgcgtatgca
     2701 ctggcacccc gtactgcaga agggataggt tcgctgaaac ttggaagcgc tgcaagtgat
     2761 ggccgccatc catttaggca tcaggtcggc ccatgaatga actccgcgat atgcttcatc
     2821 tactcgcgac agagctctgc tctacgtagt acatagtacg tgatggcttg caaggagtcc
     2881 caaggcttcc atcggtctga ctcttccgtt ccaaggttct ttgtttgcac acctcagctt
     2941 tgcctgtact gtatgttcca caatcatacc agatcgtatc gtggcgggca cttaacaagg
     3001 gcgagctcaa atcgcagcat cccattggcc agatcgccca cagccactaa ggcgctcagg
     3061 cgaggaaaaa aaaaattctt gcgggtttgc ggtgcgtttg agtgggaaac ggcagcgtag
     3121 aggaaaatat cattcgttgg ggctcgctcc ttagggctag gccagcaaac ggacgccgct
     3181 tattttgccc ctccttaagg cttttttttt ttcacaagcc ccgtttgctg ccccgcgtgg
     3241 cagaaaaaaa aaagcacagc aatagcgctg cacacccctc cgcccaccac cttttaaatc
     3301 tcctcctccc accatctgct ttcgaaattt tcttccttct gctcttcgtc tcgcataccc
     3361 ggttcaagca tccgatctgc gaatttgtat gtgctgcctc tccctctgcc ttcgccttgg
     3421 tgattgcatc ttccaacacc tggatcatca tcaccatcat cgtcggaaga acctctcgag
     3481 ctgactctgc ttgcctccag tcaatttagt caaggactac acagctctca cgctacattt
     3541 cacaaaccgc aaaaaatgtg agaccgggta ccgagctcga attcactggc cgtcgtttta
     3601 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     3661 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     3721 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     3781 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     3841 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     3901 tccgcttaca gacaagctgt gaccatctcc gggagctgca tgtgtcagag gttttcaccg
     3961 tcatcaccga aacgcgcgag tcgaaagggc ctcgtgatac gcctattttt ataggttaat
     4021 gtcatgataa taatggtttc ttagacgt
//
