LOCUS       pTrichoGate-4           3416 bp    DNA     circular SYN 08-SEP-2020
DEFINITION  MN007108 Cloning vector pTrichoGate-4, complete sequence.
ACCESSION   MN007108
VERSION     MN007108.1
KEYWORDS    .
SOURCE      Cloning vector pTrichoGate-4
  ORGANISM  Cloning vector pTrichoGate-4
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Nogueira-Lopez,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd,
            Lincoln, Christchurch, Canterbury 7647, New Zealand
COMMENT     ##Imported_From-START##
            Path     :: C:\Users\mendozaa\Geneious 2020.1 Data\temp\31
            Filename :: Clipboard Contents 9
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Cloning vector pTrichoGate-4"
                     /mol_type="other DNA"
                     /db_xref="taxon:2604824"
                     /lab_host="Escherichia coli TOP10F'"
     gene            1..711
                     /gene="mCherry"
     CDS             1..711
                     /gene="mCherry"
                     /codon_start=1
                     /transl_table=11
                     /product="codon-optimized mCherry"
                     /protein_id="QEG99021.1"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK"
     gene            complement(1934..2794)
                     /gene="AmpR"
     CDS             complement(1934..2794)
                     /gene="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="ampicillin resistance"
                     /protein_id="QEG99020.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 atggtcagca agggcgaaga agacaacatg gctattatca aggagtttat gcgattcaag
       61 gttcacatgg aaggcagcgt caacggccac gagttcgaaa tcgagggtga aggcgagggc
      121 cgaccctacg agggcaccca gactgccaag ctcaaggtca ccaagggcgg tcccctccct
      181 ttcgcttggg acatcctgtc tccacagttc atgtacggta gcaaggccta tgttaagcat
      241 ccagctgaca ttcccgatta cctcaagctg tccttccctg aaggctttaa gtgggagcgt
      301 gtcatgaact ttgaagatgg cggtgtcgtt accgttactc aggactctag ccttcaagat
      361 ggcgagttca tctacaaggt caagctgcgc ggcaccaact ttccttcaga cggcccagtt
      421 atgcagaaga agactatggg ctgggaagcc tcctcagagc gcatgtatcc tgaagatggt
      481 gctctgaagg gcgagattaa gcagcgactt aagttgaagg acggcggtca ctacgatgcc
      541 gaggtcaaga ccacttataa ggctaagaag cccgtccaac ttcctggcgc ctacaacgtc
      601 aacatcaagt tggacattac ctcccataac gaagattata ctattgttga gcaatatgag
      661 cgagccgagg gccgacacag cactggtggt atggacgaac tctacaagta agcttagaga
      721 ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc
      781 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
      841 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
      901 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      961 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1021 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1081 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1141 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1201 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1261 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1321 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1381 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1441 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1501 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1561 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     1621 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1681 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1741 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1801 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1861 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1921 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1981 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2041 taccatctgg ccccagtgct gcaatgatac cgcgagaacc acgctcaccg gctccagatt
     2101 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2161 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2221 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2281 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2341 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2401 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2461 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     2521 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     2581 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     2641 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2701 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2761 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2821 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2881 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     2941 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgactcgcgc
     3001 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagatg gtcacagctt
     3061 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     3121 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     3181 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     3241 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     3301 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     3361 agtcacgacg ttgtaaaacg acggccagtg aattcgagct cggtacccgg tctcta
//
