LOCUS       pTrichoGate-5           3443 bp    DNA     circular SYN 08-SEP-2020
DEFINITION  MN007109 Cloning vector pTrichoGate-5, complete sequence.
ACCESSION   MN007109
VERSION     MN007109.1
KEYWORDS    .
SOURCE      Cloning vector pTrichoGate-5
  ORGANISM  Cloning vector pTrichoGate-5
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Nogueira-Lopez,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd,
            Lincoln, Christchurch, Canterbury 7647, New Zealand
COMMENT     ##Imported_From-START##
            Path     :: C:\Users\mendozaa\Geneious 2020.1 Data\temp\43
            Filename :: Clipboard Contents 13
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Cloning vector pTrichoGate-5"
                     /mol_type="other DNA"
                     /db_xref="taxon:2604825"
                     /lab_host="Escherichia coli TOP10F'"
     gene            18..746
                     /gene="meGFP"
     CDS             18..746
                     /gene="meGFP"
                     /codon_start=1
                     /transl_table=11
                     /product="codon-optimized eGFP"
                     /protein_id="QEG99023.1"
                     /translation="MATMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG
                     KLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
                     FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADK
                     QKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEK
                     RDHMVLLEFVTAAGITLGMDELYK"
     gene            complement(1969..2829)
                     /gene="AmpR"
     CDS             complement(1969..2829)
                     /gene="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="ampicillin resistance"
                     /protein_id="QEG99022.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 ggtctctaat gctcgagatg gctactatgg tcagcaaggg cgaagaactt tttactggtg
       61 tcgtcccaat cctggtcgaa ctggatggcg atgtcaacgg tcataagttc tctgttagcg
      121 gcgagggtga aggcgacgct acctacggca agctcacttt gaagtttatc tgcaccactg
      181 gcaagctccc cgtcccttgg ccaaccctcg ttaccactct gacttacggc gttcagtgct
      241 tcagccgcta tccagaccac atgaagcaac atgatttctt taagtccgcc atgcccgagg
      301 gttacgtcca ggaacgcacc atcttcttta aggacgatgg caactataag acccgagctg
      361 aggtcaagtt cgaaggtgac actctcgtta accgtatcga gctgaagggc attgacttta
      421 aggaagatgg taacattctt ggccacaagt tggagtacaa ctataactca cataacgtct
      481 acatcatggc cgataagcag aagaacggta ttaaggttaa cttcaagatc cgccacaaca
      541 ttgaagacgg ctccgtccaa ctggctgatc attaccagca aaacacccct attggtgacg
      601 gccccgttct cctgcctgat aaccactatc tttccactca gtcagctttg tctaaggacc
      661 caaacgagaa gcgagatcac atggtcctgc tggagtttgt cactgctgct ggtatcaccc
      721 tgggcatgga cgagttgtat aagtaagctt agagaccggg gatcctctag agtcgacctg
      781 caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      841 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      901 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      961 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1021 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1081 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     1141 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1201 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1261 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1321 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1381 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1441 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1501 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1561 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1621 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     1681 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1741 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1801 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1861 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1921 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1981 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2041 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2101 gataccgcga gaaccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2161 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2221 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2281 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2341 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2401 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2461 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2521 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2581 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2641 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2701 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2761 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2821 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     2881 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2941 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     3001 aaataggcgt atcacgaggc cctttcgact cgcgcgtttc ggtgatgacg gtgaaaacct
     3061 ctgacacatg cagctcccgg agatggtcac agcttgtctg taagcggatg ccgggagcag
     3121 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     3181 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     3241 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     3301 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     3361 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     3421 cagtgaattc gagctcggta ccc
//
