LOCUS       pTrichoGate-7           3449 bp    DNA     circular SYN 08-SEP-2020
DEFINITION  MN007111 Cloning vector pTrichoGate-7, complete sequence.
ACCESSION   MN007111
VERSION     MN007111.1
KEYWORDS    .
SOURCE      Cloning vector pTrichoGate-7
  ORGANISM  Cloning vector pTrichoGate-7
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Nogueira-Lopez,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd,
            Lincoln, Christchurch, Canterbury 7647, New Zealand
COMMENT     ##Imported_From-START##
            Path     :: C:\Users\mendozaa\Geneious 2020.1 Data\temp\19
            Filename :: Clipboard Contents 5
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Cloning vector pTrichoGate-7"
                     /mol_type="other DNA"
                     /db_xref="taxon:2604827"
                     /lab_host="Escherichia coli TOP10F'"
     gene            1..708
                     /gene="mCherry"
     misc_feature    1..708
                     /gene="mCherry"
                     /note="codon-optimized mCherry; linker localized in
                     C-teminal of mCheery for N-teminal fusion"
     misc_feature    709..744
                     /note="flexible linker GSAGSAAGSGEF"
     gene            1370..2230
                     /gene="AmpR"
     CDS             1370..2230
                     /gene="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="ampicillin resistance"
                     /protein_id="QEG99027.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 atggtcagca agggcgaaga agacaacatg gctattatca aggagtttat gcgattcaag
       61 gttcacatgg aaggcagcgt caacggccac gagttcgaaa tcgagggtga aggcgagggc
      121 cgaccctacg agggcaccca gactgccaag ctcaaggtca ccaagggcgg tcccctccct
      181 ttcgcttggg acatcctgtc tccacagttc atgtacggta gcaaggccta tgttaagcat
      241 ccagctgaca ttcccgatta cctcaagctg tccttccctg aaggctttaa gtgggagcgt
      301 gtcatgaact ttgaagatgg cggtgtcgtt accgttactc aggactctag ccttcaagat
      361 ggcgagttca tctacaaggt caagctgcgc ggcaccaact ttccttcaga cggcccagtt
      421 atgcagaaga agactatggg ctgggaagcc tcctcagagc gcatgtatcc tgaagatggt
      481 gctctgaagg gcgagattaa gcagcgactt aagttgaagg acggcggtca ctacgatgcc
      541 gaggtcaaga ccacttataa ggctaagaag cccgtccaac ttcctggcgc ctacaacgtc
      601 aacatcaagt tggacattac ctcccataac gaagattata ctattgttga gcaatatgag
      661 cgagccgagg gccgacacag cactggtggt atggacgaac tctacaaggg ttctgctggt
      721 tctgctgctg gttctggtga attcgctatg agaccgggta ccgagctcga attcactggc
      781 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      841 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      901 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
      961 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     1021 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     1081 gctcccggca tccgcttaca gacaagctgt gaccatctcc gggagctgca tgtgtcagag
     1141 gttttcaccg tcatcaccga aacgcgcgag tcgaaagggc ctcgtgatac gcctattttt
     1201 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     1261 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1321 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     1381 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     1441 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     1501 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     1561 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     1621 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     1681 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     1741 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     1801 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1861 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1921 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1981 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2041 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggttctc gcggtatcat
     2101 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2161 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2221 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2281 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2341 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2401 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2461 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2521 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     2581 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2641 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2701 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2761 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2821 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2881 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2941 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3001 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3061 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3121 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     3181 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     3241 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     3301 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     3361 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca
     3421 ggtcgactct agaggatccc cggtctcta
//
