LOCUS       pTrichoGate-8           3476 bp    DNA     circular SYN 08-SEP-2020
DEFINITION  MN007112 Cloning vector pTrichoGate-8, complete sequence.
ACCESSION   MN007112
VERSION     MN007112.1
KEYWORDS    .
SOURCE      Cloning vector pTrichoGate-8
  ORGANISM  Cloning vector pTrichoGate-8
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Nogueira-Lopez,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd,
            Lincoln, Christchurch, Canterbury 7647, New Zealand
COMMENT     ##Imported_From-START##
            Path     :: C:\Users\mendozaa\Geneious 2020.1 Data\temp\40
            Filename :: Clipboard Contents 12
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Cloning vector pTrichoGate-8"
                     /mol_type="other DNA"
                     /db_xref="taxon:2604828"
                     /lab_host="Escherichia coli TOP10F'"
     gene            18..743
                     /gene="eGFP"
     misc_feature    18..743
                     /gene="eGFP"
                     /note="codon-optimized eGFP; linker localized in
                     C-teminalof eGFP for N-teminal fusion"
     misc_feature    744..780
                     /note="flexible linker GSAGSAAGSGEF"
     gene            complement(2002..2862)
                     /gene="AmpR"
     CDS             complement(2002..2862)
                     /gene="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="ampicillin resistance"
                     /protein_id="QEG99028.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 ggtctctaat gctcgagatg gctactatgg tcagcaaggg cgaagaactt tttactggtg
       61 tcgtcccaat cctggtcgaa ctggatggcg atgtcaacgg tcataagttc tctgttagcg
      121 gcgagggtga aggcgacgct acctacggca agctcacttt gaagtttatc tgcaccactg
      181 gcaagctccc cgtcccttgg ccaaccctcg ttaccactct gacttacggc gttcagtgct
      241 tcagccgcta tccagaccac atgaagcaac atgatttctt taagtccgcc atgcccgagg
      301 gttacgtcca ggaacgcacc atcttcttta aggacgatgg caactataag acccgagctg
      361 aggtcaagtt cgaaggtgac actctcgtta accgtatcga gctgaagggc attgacttta
      421 aggaagatgg taacattctt ggccacaagt tggagtacaa ctataactca cataacgtct
      481 acatcatggc cgataagcag aagaacggta ttaaggttaa cttcaagatc cgccacaaca
      541 ttgaagacgg ctccgtccaa ctggctgatc attaccagca aaacacccct attggtgacg
      601 gccccgttct cctgcctgat aaccactatc tttccactca gtcagctttg tctaaggacc
      661 caaacgagaa gcgagatcac atggtcctgc tggagtttgt cactgctgct ggtatcaccc
      721 tgggcatgga cgagttgtat aagggttctg ctggttctgc tgctggttct ggtgaattcg
      781 ctatgagacc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg
      841 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      901 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      961 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1021 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1081 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1141 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1201 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1261 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1321 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1381 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1441 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1501 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1561 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1621 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1681 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1741 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1801 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1861 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1921 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1981 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2041 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2101 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagaaccac gctcaccggc
     2161 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2221 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2281 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2341 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2401 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2461 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2521 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2581 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     2641 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2701 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2761 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2821 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2881 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2941 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     3001 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     3061 actcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagatggt
     3121 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     3181 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     3241 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     3301 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3361 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     3421 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcgagctcg gtaccc
//
