LOCUS       pTrichoGate-11          3472 bp    DNA     circular SYN 08-SEP-2020
DEFINITION  MN007115 Cloning vector pTrichoGate-11, complete sequence.
ACCESSION   MN007115
VERSION     MN007115.1
KEYWORDS    .
SOURCE      Cloning vector pTrichoGate-11
  ORGANISM  Cloning vector pTrichoGate-11
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Nogueira-Lopez,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd,
            Lincoln, Christchurch, Canterbury 7647, New Zealand
COMMENT     ##Imported_From-START##
            Path     :: C:\Users\mendozaa\Geneious 2020.1 Data\temp\25
            Filename :: Clipboard Contents 7
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Cloning vector pTrichoGate-11"
                     /mol_type="other DNA"
                     /db_xref="taxon:2604811"
                     /lab_host="Escherichia coli TOP10F'"
     misc_feature    11..46
                     /note="flexible linker GSAGSAAGSGEF"
     gene            47..775
                     /gene="eGFP"
     misc_feature    47..775
                     /gene="eGFP"
                     /note="codon-optimized eGFP; linker localized in
                     C-teminalof mCherry for N-teminal fusion"
     gene            1401..2261
                     /gene="AmpR"
     CDS             1401..2261
                     /gene="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="ampicillin resistance"
                     /protein_id="QEG99032.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 ggtctcagcc ggatctgctg gttctgctgc tggttctggt gaattcatgg ctactatggt
       61 cagcaagggc gaagaacttt ttactggtgt cgtcccaatc ctggtcgaac tggatggcga
      121 tgtcaacggt cataagttct ctgttagcgg cgagggtgaa ggcgacgcta cctacggcaa
      181 gctcactttg aagtttatct gcaccactgg caagctcccc gtcccttggc caaccctcgt
      241 taccactctg acttacggcg ttcagtgctt cagccgctat ccagaccaca tgaagcaaca
      301 tgatttcttt aagtccgcca tgcccgaggg ttacgtccag gaacgcacca tcttctttaa
      361 ggacgatggc aactataaga cccgagctga ggtcaagttc gaaggtgaca ctctcgttaa
      421 ccgtatcgag ctgaagggca ttgactttaa ggaagatggt aacattcttg gccacaagtt
      481 ggagtacaac tataactcac ataacgtcta catcatggcc gataagcaga agaacggtat
      541 taaggttaac ttcaagatcc gccacaacat tgaagacggc tccgtccaac tggctgatca
      601 ttaccagcaa aacaccccta ttggtgacgg ccccgttctc ctgcctgata accactatct
      661 ttccactcag tcagctttgt ctaaggaccc aaacgagaag cgagatcaca tggtcctgct
      721 ggagtttgtc actgctgctg gtatcaccct gggcatggac gagttgtata agtaagctta
      781 gagaccgggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      841 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      901 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      961 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     1021 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     1081 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     1141 tgaccatctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     1201 gtcgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1261 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1321 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1381 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1441 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1501 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1561 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1621 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     1681 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     1741 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     1801 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1861 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1921 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     1981 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2041 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2101 gagccggtga gcgtggttct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2161 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2221 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2281 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2341 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2401 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2461 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2521 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2581 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2641 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2701 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2761 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2821 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2881 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2941 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3001 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3061 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3121 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3181 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3241 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3301 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3361 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3421 accatgatta cgccaagctt gcatgcctgc aggtcgactc tagaggatcc cc
//
