LOCUS       pTrichoGate-13          2818 bp    DNA     circular SYN 08-SEP-2020
DEFINITION  MN007117 Cloning vector pTrichoGate-13, complete sequence.
ACCESSION   MN007117
VERSION     MN007117.1
KEYWORDS    .
SOURCE      Cloning vector pTrichoGate-13
  ORGANISM  Cloning vector pTrichoGate-13
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Nogueira-Lopez,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd,
            Lincoln, Christchurch, Canterbury 7647, New Zealand
COMMENT     ##Imported_From-START##
            Path     :: C:\Users\mendozaa\Geneious 2020.1 Data\temp\7
            Filename :: Clipboard Contents
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Cloning vector pTrichoGate-13"
                     /mol_type="other DNA"
                     /db_xref="taxon:2604813"
                     /lab_host="Escherichia coli TOP10F'"
     gene            1..102
                     /gene="3XHA-tag"
     misc_feature    1..102
                     /gene="3XHA-tag"
                     /note="codon-optimized HA-tag"
     gene            complement(1334..2194)
                     /gene="AmpR"
     CDS             complement(1334..2194)
                     /gene="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="ampicillin resistance"
                     /protein_id="QEG99035.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 gcctacccct atgacgtccc tgattacgct ggtgctgctt acccctacga cgttcccgat
       61 tattctgctg gcggttaccc ttatgacgtc ccagattact agggcgcgcc ggcttagaga
      121 ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc
      181 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
      241 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
      301 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      361 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      421 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      481 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      541 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      601 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      661 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      721 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      781 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      841 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      901 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      961 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     1021 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1081 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1141 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1201 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1261 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1321 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1381 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1441 taccatctgg ccccagtgct gcaatgatac cgcgagaacc acgctcaccg gctccagatt
     1501 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1561 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1621 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1681 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1741 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1801 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1861 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1921 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1981 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     2041 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2101 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2161 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2221 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2281 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     2341 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgactcgcgc
     2401 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagatg gtcacagctt
     2461 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2521 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2581 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2641 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2701 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2761 agtcacgacg ttgtaaaacg acggccagtg aattcgagct cggtacccgg tctcagcc
//
