LOCUS       pTrichoGate-15          2829 bp    DNA     circular SYN 08-SEP-2020
DEFINITION  MN007119 Cloning vector pTrichoGate-15, complete sequence.
ACCESSION   MN007119
VERSION     MN007119.1
KEYWORDS    .
SOURCE      Cloning vector pTrichoGate-15
  ORGANISM  Cloning vector pTrichoGate-15
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Nogueira-Lopez,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd,
            Lincoln, Christchurch, Canterbury 7647, New Zealand
COMMENT     ##Imported_From-START##
            Path     :: C:\Users\mendozaa\Geneious 2020.1 Data\temp\16
            Filename :: Clipboard Contents 4
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Cloning vector pTrichoGate-15"
                     /mol_type="other DNA"
                     /db_xref="taxon:2604815"
                     /lab_host="Escherichia coli TOP10F'"
     gene            1..117
                     /gene="3XFLAG-tag"
     misc_feature    1..117
                     /gene="3XFLAG-tag"
                     /note="codon-optimized 3XFLAG-tag"
     gene            complement(1348..2208)
                     /gene="AmpR"
     CDS             complement(1348..2208)
                     /gene="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="ampicillin resistance"
                     /protein_id="QEG99037.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 gccgactaca aggacgatga cgataagatc gccggcgctg gttctgatta taaggatgac
       61 gacgataaga ttagcgccgg cggtgctgat tataaggatg atgatgataa gatctagggc
      121 gcgccgctta gagaccgggg atcctctaga gtcgacctgc aggcatgcaa gcttggcgta
      181 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      241 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      301 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      361 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      421 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      481 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      541 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      601 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      661 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      721 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      781 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      841 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      901 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      961 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1021 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1081 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1141 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1201 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1261 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1321 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     1381 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     1441 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag aaccacgctc
     1501 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     1561 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     1621 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     1681 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1741 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1801 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     1861 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     1921 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     1981 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2041 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2101 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2161 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2221 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2281 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     2341 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     2401 ctttcgactc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     2461 gatggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     2521 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     2581 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     2641 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     2701 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     2761 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg agctcggtac
     2821 ccggtctca
//
