LOCUS       pTrichoGate-16          3008 bp    DNA     circular SYN 08-SEP-2020
DEFINITION  MN007120 Cloning vector pTrichoGate-16, complete sequence.
ACCESSION   MN007120
VERSION     MN007120.1
KEYWORDS    .
SOURCE      Cloning vector pTrichoGate-16
  ORGANISM  Cloning vector pTrichoGate-16
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Nogueira-Lopez,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd,
            Lincoln, Christchurch, Canterbury 7647, New Zealand
COMMENT     ##Imported_From-START##
            Path     :: C:\Users\mendozaa\Geneious 2020.1 Data\temp\37
            Filename :: Clipboard Contents 11
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Cloning vector pTrichoGate-16"
                     /mol_type="other DNA"
                     /db_xref="taxon:2604816"
                     /lab_host="Escherichia coli TOP10F'"
     regulatory      20..311
                     /regulatory_class="terminator"
                     /note="nopaline synthase terminator (T-nos)"
     gene            complement(1534..2394)
                     /gene="AmpR"
     CDS             complement(1534..2394)
                     /gene="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="ampicillin resistance"
                     /protein_id="QEG99038.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 ggtctctgct tggcgcgccg gccgcccggc gatcgttcaa acatttggca ataaagtttc
       61 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
      121 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
      181 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
      241 taggataaat tatcgcgcgc ggtgtcatct atgttactag atccgatgat aagctgtcaa
      301 acatgagaat ttaacggaga ccggggatcc tctagagtcg acctgcaggc atgcaagctt
      361 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      421 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      481 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      541 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      601 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      661 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      721 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      781 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      841 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      901 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      961 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1021 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1081 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1141 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1201 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1261 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1321 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1381 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1441 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1501 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1561 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1621 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaacc
     1681 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1741 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1801 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1861 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1921 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1981 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2041 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2101 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2161 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2221 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2281 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2341 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2401 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2461 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2521 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2581 gaggcccttt cgactcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2641 cccggagatg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2701 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2761 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2821 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2881 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2941 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattcgagct
     3001 cggtaccc
//
