LOCUS       pTrichoGate-17          3260 bp    DNA     circular SYN 08-SEP-2020
DEFINITION  MN007121 Cloning vector pTrichoGate-17, complete sequence.
ACCESSION   MN007121
VERSION     MN007121.1
KEYWORDS    .
SOURCE      Cloning vector pTrichoGate-17
  ORGANISM  Cloning vector pTrichoGate-17
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Nogueira-Lopez,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd,
            Lincoln, Christchurch, Canterbury 7647, New Zealand
COMMENT     ##Imported_From-START##
            Path     :: C:\Users\mendozaa\Geneious 2020.1 Data\temp\28
            Filename :: Clipboard Contents 8
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Cloning vector pTrichoGate-17"
                     /mol_type="other DNA"
                     /db_xref="taxon:2604817"
                     /lab_host="Escherichia coli TOP10F'"
     regulatory      1..574
                     /regulatory_class="terminator"
                     /note="tryptophan synthase terminator (TtrpC)"
     gene            1189..2049
                     /gene="AmpR"
     CDS             1189..2049
                     /gene="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="ampicillin resistance"
                     /protein_id="QEG99039.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
ORIGIN      
        1 ggtctctgct tctagtgatt taatagctcc atgtcaacaa gaataaaacg cgtttcgggt
       61 ttacctcttc cagatacagc tcatctgcaa tgcattaatg cattggacct cgcaacccta
      121 gtacgccctt caggctccgg cgaagcagaa gaatagctta gcagagtcta ttttcatttt
      181 cgggagacga gatcaagcag atcaacggtc gtcaagagac ctacgagact gaggaatccg
      241 ctcttggctc cacgcgacta tatatttgtc tctaattgta ctttgacatg ctcctcttct
      301 ttactctgat agcttgacta tgaaaattcc gtcaccagcc cctgggttcg caaagataat
      361 tgcactgttt cttccttgaa ctctcaagcc tacaggacac acattcatcg taggtataaa
      421 cctcgaaaat cattcctact aagatgggta tacaatagta accatgcatg gttgcctagt
      481 gaatgctccg taacacccaa tacgccggcc gaaacttttt tacaactctc ctatgagtcg
      541 tttacccaga atgcacaggt acctaacgga gaccgggtac cgagctcgaa ttcactggcc
      601 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      661 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      721 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
      781 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
      841 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
      901 ctcccggcat ccgcttacag acaagctgtg accatctccg ggagctgcat gtgtcagagg
      961 ttttcaccgt catcaccgaa acgcgcgagt cgaaagggcc tcgtgatacg cctattttta
     1021 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     1081 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1141 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1201 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1261 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1321 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1381 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     1441 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1501 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1561 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1621 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1681 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1741 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1801 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1861 gctggctggt ttattgctga taaatctgga gccggtgagc gtggttctcg cggtatcatt
     1921 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1981 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2041 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2101 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2161 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2221 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2281 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2341 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     2401 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2461 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2521 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2581 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2641 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2701 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2761 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2821 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2881 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2941 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3001 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3061 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     3121 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     3181 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc atgcctgcag
     3241 gtcgactcta gaggatcccc
//
