LOCUS       Exported                5217 bp ds-DNA     circular SYN 18-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Xinyi Wan
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 18, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(59..78)
                     /label=VR
     CDS             complement(141..956)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(1551..2095)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2513..2532
                     /label=VF2
     terminator      2585..2628
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2631..2652
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        2693..2750
                     /label=Ptet2
     protein_bind    2693..2711
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2718..2736
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2813..2870
                     /label=Ptet2
     protein_bind    2813..2831
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2838..2856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2933..2990
                     /label=Ptet2
     protein_bind    2933..2951
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2958..2976
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3053..3110
                     /label=Ptet2
     protein_bind    3053..3071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3078..3096
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3173..3230
                     /label=Ptet2
     protein_bind    3173..3191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3198..3216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3297..3354
                     /label=Ptet2
     protein_bind    3297..3315
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3322..3340
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3417..3474
                     /label=Ptet2
     protein_bind    3417..3435
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3442..3460
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3537..3594
                     /label=Ptet2
     protein_bind    3537..3555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3562..3580
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3657..3714
                     /label=Ptet2
     protein_bind    3657..3675
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3682..3700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3777..3834
                     /label=Ptet2
     protein_bind    3777..3795
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3802..3820
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3901..3958
                     /label=Ptet2
     protein_bind    3901..3919
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3926..3944
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4021..4078
                     /label=Ptet2
     protein_bind    4021..4039
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4046..4064
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4141..4198
                     /label=Ptet2
     protein_bind    4141..4159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4166..4184
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4261..4318
                     /label=Ptet2
     protein_bind    4261..4279
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4286..4304
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4381..4438
                     /label=Ptet2
     protein_bind    4381..4399
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4406..4424
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4505..4562
                     /label=Ptet2
     protein_bind    4505..4523
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4530..4548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4625..4682
                     /label=Ptet2
     protein_bind    4625..4643
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4650..4668
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4745..4802
                     /label=Ptet2
     protein_bind    4745..4763
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4770..4788
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4865..4922
                     /label=Ptet2
     protein_bind    4865..4883
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4890..4908
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4985..5042
                     /label=Ptet2
     protein_bind    4985..5003
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5010..5028
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      5069..5197
                     /label=B0015
     misc_feature    join(5198..5217,1)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
       61 tcactcaaag gcggtaatct cgagtcccgt caagtcagcg taatgctctg ccagtgttac
      121 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
      181 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
      241 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
      301 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
      361 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc
      421 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
      481 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
      541 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
      601 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca
      661 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
      721 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
      781 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
      841 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
      901 atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg gctcataaca
      961 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     1021 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
     1081 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
     1141 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
     1201 ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca
     1261 ccttcttcac gaggcagacc tcagcgctag cggagtgtat actggcttac tatgttggca
     1321 ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg
     1381 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc
     1441 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga
     1501 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
     1561 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
     1621 acaggactat aaagatacca ggcgtttccc ctggcggctc cctcgtgcgc tctcctgttc
     1681 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc
     1741 ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc
     1801 gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga
     1861 catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa
     1921 gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag
     1981 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa
     2041 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga
     2101 tcatcttatt aaggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2401 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattccat ggtgccacct
     2521 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2581 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
     2641 ccgcttctag agatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     2701 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     2761 cctagaatga gtatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     2821 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     2881 cctagaattt ccatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     2941 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     3001 cctagaatga ctatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     3061 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     3121 cctagaattg atatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     3181 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     3241 cctagaatta ctagagatcg attttagagc tgccttttca gcaggacgca ctgacctccc
     3301 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac atatcttagg
     3361 tgtccctaga atgagtatcg attttagagc tgccttttca gcaggacgca ctgacctccc
     3421 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac atatcttagg
     3481 tgtccctaga atttccatcg attttagagc tgccttttca gcaggacgca ctgacctccc
     3541 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac atatcttagg
     3601 tgtccctaga atgactatcg attttagagc tgccttttca gcaggacgca ctgacctccc
     3661 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac atatcttagg
     3721 tgtccctaga attgatatcg attttagagc tgccttttca gcaggacgca ctgacctccc
     3781 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac atatcttagg
     3841 tgtccctaga attactagag atcgatttta gagctgcctt ttcagcagga cgcactgacc
     3901 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatatct
     3961 taggtgtccc tagaatgagt atcgatttta gagctgcctt ttcagcagga cgcactgacc
     4021 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatatct
     4081 taggtgtccc tagaatttcc atcgatttta gagctgcctt ttcagcagga cgcactgacc
     4141 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatatct
     4201 taggtgtccc tagaatgact atcgatttta gagctgcctt ttcagcagga cgcactgacc
     4261 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatatct
     4321 taggtgtccc tagaattgat atcgatttta gagctgcctt ttcagcagga cgcactgacc
     4381 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatatct
     4441 taggtgtccc tagaattact agagatcgat tttagagctg ccttttcagc aggacgcact
     4501 gacctcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacat
     4561 atcttaggtg tccctagaat gagtatcgat tttagagctg ccttttcagc aggacgcact
     4621 gacctcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacat
     4681 atcttaggtg tccctagaat ttccatcgat tttagagctg ccttttcagc aggacgcact
     4741 gacctcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacat
     4801 atcttaggtg tccctagaat gactatcgat tttagagctg ccttttcagc aggacgcact
     4861 gacctcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacat
     4921 atcttaggtg tccctagaat tgatatcgat tttagagctg ccttttcagc aggacgcact
     4981 gacctcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacat
     5041 atcttaggtg tccctagaat tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     5101 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     5161 actggctcac cttcgggtgg gcctttctgc gtttatatac tagtagcggc cgctgca
//
