LOCUS       Exported                7633 bp ds-DNA     circular SYN 17-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Xinyi Wan
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 18, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(59..78)
                     /label=VR
     CDS             complement(141..956)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(1551..2095)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2513..2532
                     /label=VF2
     terminator      2585..2628
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2631..2652
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        2693..2750
                     /label=Ptet2
     protein_bind    2693..2711
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2718..2736
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2813..2870
                     /label=Ptet2
     protein_bind    2813..2831
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2838..2856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2933..2990
                     /label=Ptet2
     protein_bind    2933..2951
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2958..2976
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3053..3110
                     /label=Ptet2
     protein_bind    3053..3071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3078..3096
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3173..3230
                     /label=Ptet2
     protein_bind    3173..3191
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3198..3216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3297..3354
                     /label=Ptet2
     protein_bind    3297..3315
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3322..3340
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3417..3474
                     /label=Ptet2
     protein_bind    3417..3435
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3442..3460
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3537..3594
                     /label=Ptet2
     protein_bind    3537..3555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3562..3580
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3657..3714
                     /label=Ptet2
     protein_bind    3657..3675
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3682..3700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3777..3834
                     /label=Ptet2
     protein_bind    3777..3795
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3802..3820
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3901..3958
                     /label=Ptet2
     protein_bind    3901..3919
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3926..3944
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4021..4078
                     /label=Ptet2
     protein_bind    4021..4039
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4046..4064
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4141..4198
                     /label=Ptet2
     protein_bind    4141..4159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4166..4184
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4261..4318
                     /label=Ptet2
     protein_bind    4261..4279
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4286..4304
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4381..4438
                     /label=Ptet2
     protein_bind    4381..4399
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4406..4424
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4505..4562
                     /label=Ptet2
     protein_bind    4505..4523
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4530..4548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4625..4682
                     /label=Ptet2
     protein_bind    4625..4643
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4650..4668
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4745..4802
                     /label=Ptet2
     protein_bind    4745..4763
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4770..4788
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4865..4922
                     /label=Ptet2
     protein_bind    4865..4883
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4890..4908
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4985..5042
                     /label=Ptet2
     protein_bind    4985..5003
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5010..5028
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5109..5166
                     /label=Ptet2
     protein_bind    5109..5127
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5134..5152
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5229..5286
                     /label=Ptet2
     protein_bind    5229..5247
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5254..5272
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5349..5406
                     /label=Ptet2
     protein_bind    5349..5367
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5374..5392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5469..5526
                     /label=Ptet2
     protein_bind    5469..5487
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5494..5512
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5589..5646
                     /label=Ptet2
     protein_bind    5589..5607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5614..5632
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5713..5770
                     /label=Ptet2
     protein_bind    5713..5731
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5738..5756
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5833..5890
                     /label=Ptet2
     protein_bind    5833..5851
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5858..5876
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5953..6010
                     /label=Ptet2
     protein_bind    5953..5971
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5978..5996
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6073..6130
                     /label=Ptet2
     protein_bind    6073..6091
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6098..6116
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6193..6250
                     /label=Ptet2
     protein_bind    6193..6211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6218..6236
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6317..6374
                     /label=Ptet2
     protein_bind    6317..6335
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6342..6360
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6437..6494
                     /label=Ptet2
     protein_bind    6437..6455
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6462..6480
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6557..6614
                     /label=Ptet2
     protein_bind    6557..6575
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6582..6600
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6677..6734
                     /label=Ptet2
     protein_bind    6677..6695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6702..6720
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6797..6854
                     /label=Ptet2
     protein_bind    6797..6815
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6822..6840
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        6921..6978
                     /label=Ptet2
     protein_bind    6921..6939
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6946..6964
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        7041..7098
                     /label=Ptet2
     protein_bind    7041..7059
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7066..7084
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        7161..7218
                     /label=Ptet2
     protein_bind    7161..7179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7186..7204
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        7281..7338
                     /label=Ptet2
     protein_bind    7281..7299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7306..7324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        7401..7458
                     /label=Ptet2
     protein_bind    7401..7419
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7426..7444
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      7485..7613
                     /label=B0015
     misc_feature    join(7614..7633,1)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
       61 tcactcaaag gcggtaatct cgagtcccgt caagtcagcg taatgctctg ccagtgttac
      121 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
      181 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
      241 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
      301 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
      361 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc
      421 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
      481 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
      541 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
      601 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca
      661 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
      721 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
      781 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
      841 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
      901 atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg gctcataaca
      961 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     1021 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
     1081 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
     1141 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
     1201 ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca
     1261 ccttcttcac gaggcagacc tcagcgctag cggagtgtat actggcttac tatgttggca
     1321 ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg
     1381 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc
     1441 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga
     1501 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
     1561 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
     1621 acaggactat aaagatacca ggcgtttccc ctggcggctc cctcgtgcgc tctcctgttc
     1681 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc
     1741 ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc
     1801 gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga
     1861 catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa
     1921 gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag
     1981 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa
     2041 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga
     2101 tcatcttatt aaggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2401 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattccat ggtgccacct
     2521 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2581 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
     2641 ccgcttctag agatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     2701 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     2761 cctagaatga gtatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     2821 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     2881 cctagaattt ccatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     2941 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     3001 cctagaatga ctatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     3061 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     3121 cctagaattg atatcgattt tagagctgcc ttttcagcag gacgcactga cctccctatc
     3181 agtgatagag attgacatcc ctatcagtga tagagatact gagcacatat cttaggtgtc
     3241 cctagaatta ctagagatcg attttagagc tgccttttca gcaggacgca ctgacctccc
     3301 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac atatcttagg
     3361 tgtccctaga atgagtatcg attttagagc tgccttttca gcaggacgca ctgacctccc
     3421 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac atatcttagg
     3481 tgtccctaga atttccatcg attttagagc tgccttttca gcaggacgca ctgacctccc
     3541 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac atatcttagg
     3601 tgtccctaga atgactatcg attttagagc tgccttttca gcaggacgca ctgacctccc
     3661 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac atatcttagg
     3721 tgtccctaga attgatatcg attttagagc tgccttttca gcaggacgca ctgacctccc
     3781 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac atatcttagg
     3841 tgtccctaga attactagag atcgatttta gagctgcctt ttcagcagga cgcactgacc
     3901 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatatct
     3961 taggtgtccc tagaatgagt atcgatttta gagctgcctt ttcagcagga cgcactgacc
     4021 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatatct
     4081 taggtgtccc tagaatttcc atcgatttta gagctgcctt ttcagcagga cgcactgacc
     4141 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatatct
     4201 taggtgtccc tagaatgact atcgatttta gagctgcctt ttcagcagga cgcactgacc
     4261 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatatct
     4321 taggtgtccc tagaattgat atcgatttta gagctgcctt ttcagcagga cgcactgacc
     4381 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatatct
     4441 taggtgtccc tagaattact agagatcgat tttagagctg ccttttcagc aggacgcact
     4501 gacctcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacat
     4561 atcttaggtg tccctagaat gagtatcgat tttagagctg ccttttcagc aggacgcact
     4621 gacctcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacat
     4681 atcttaggtg tccctagaat ttccatcgat tttagagctg ccttttcagc aggacgcact
     4741 gacctcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacat
     4801 atcttaggtg tccctagaat gactatcgat tttagagctg ccttttcagc aggacgcact
     4861 gacctcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacat
     4921 atcttaggtg tccctagaat tgatatcgat tttagagctg ccttttcagc aggacgcact
     4981 gacctcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacat
     5041 atcttaggtg tccctagaat tactagagat cgattttaga gctgcctttt cagcaggacg
     5101 cactgacctc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
     5161 acatatctta ggtgtcccta gaatgagtat cgattttaga gctgcctttt cagcaggacg
     5221 cactgacctc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
     5281 acatatctta ggtgtcccta gaatttccat cgattttaga gctgcctttt cagcaggacg
     5341 cactgacctc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
     5401 acatatctta ggtgtcccta gaatgactat cgattttaga gctgcctttt cagcaggacg
     5461 cactgacctc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
     5521 acatatctta ggtgtcccta gaattgatat cgattttaga gctgcctttt cagcaggacg
     5581 cactgacctc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
     5641 acatatctta ggtgtcccta gaattactag agatcgattt tagagctgcc ttttcagcag
     5701 gacgcactga cctccctatc agtgatagag attgacatcc ctatcagtga tagagatact
     5761 gagcacatat cttaggtgtc cctagaatga gtatcgattt tagagctgcc ttttcagcag
     5821 gacgcactga cctccctatc agtgatagag attgacatcc ctatcagtga tagagatact
     5881 gagcacatat cttaggtgtc cctagaattt ccatcgattt tagagctgcc ttttcagcag
     5941 gacgcactga cctccctatc agtgatagag attgacatcc ctatcagtga tagagatact
     6001 gagcacatat cttaggtgtc cctagaatga ctatcgattt tagagctgcc ttttcagcag
     6061 gacgcactga cctccctatc agtgatagag attgacatcc ctatcagtga tagagatact
     6121 gagcacatat cttaggtgtc cctagaattg atatcgattt tagagctgcc ttttcagcag
     6181 gacgcactga cctccctatc agtgatagag attgacatcc ctatcagtga tagagatact
     6241 gagcacatat cttaggtgtc cctagaatta ctagagatcg attttagagc tgccttttca
     6301 gcaggacgca ctgacctccc tatcagtgat agagattgac atccctatca gtgatagaga
     6361 tactgagcac atatcttagg tgtccctaga atgagtatcg attttagagc tgccttttca
     6421 gcaggacgca ctgacctccc tatcagtgat agagattgac atccctatca gtgatagaga
     6481 tactgagcac atatcttagg tgtccctaga atttccatcg attttagagc tgccttttca
     6541 gcaggacgca ctgacctccc tatcagtgat agagattgac atccctatca gtgatagaga
     6601 tactgagcac atatcttagg tgtccctaga atgactatcg attttagagc tgccttttca
     6661 gcaggacgca ctgacctccc tatcagtgat agagattgac atccctatca gtgatagaga
     6721 tactgagcac atatcttagg tgtccctaga attgatatcg attttagagc tgccttttca
     6781 gcaggacgca ctgacctccc tatcagtgat agagattgac atccctatca gtgatagaga
     6841 tactgagcac atatcttagg tgtccctaga attactagag atcgatttta gagctgcctt
     6901 ttcagcagga cgcactgacc tccctatcag tgatagagat tgacatccct atcagtgata
     6961 gagatactga gcacatatct taggtgtccc tagaatgagt atcgatttta gagctgcctt
     7021 ttcagcagga cgcactgacc tccctatcag tgatagagat tgacatccct atcagtgata
     7081 gagatactga gcacatatct taggtgtccc tagaatttcc atcgatttta gagctgcctt
     7141 ttcagcagga cgcactgacc tccctatcag tgatagagat tgacatccct atcagtgata
     7201 gagatactga gcacatatct taggtgtccc tagaatgact atcgatttta gagctgcctt
     7261 ttcagcagga cgcactgacc tccctatcag tgatagagat tgacatccct atcagtgata
     7321 gagatactga gcacatatct taggtgtccc tagaattgat atcgatttta gagctgcctt
     7381 ttcagcagga cgcactgacc tccctatcag tgatagagat tgacatccct atcagtgata
     7441 gagatactga gcacatatct taggtgtccc tagaattact agagccaggc atcaaataaa
     7501 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     7561 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactagt
     7621 agcggccgct gca
//
