LOCUS       Exported               10001 bp ds-DNA     circular SYN 17-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10001)
  AUTHORS   Xinyi Wan
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 18, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10001
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(59..78)
                     /label=VR
     CDS             complement(141..956)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(1551..2095)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2513..2532
                     /label=VF2
     terminator      2585..2628
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2631..2652
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     regulatory      2676..2710
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      2738..2772
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      2796..2830
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      2860..2894
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      2925..2959
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3010..3044
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3072..3106
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3130..3164
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3194..3228
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3259..3293
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3344..3378
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3406..3440
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3464..3498
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3528..3562
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3593..3627
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3678..3712
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3740..3774
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3798..3832
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3862..3896
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      3927..3961
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4016..4050
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4078..4112
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4136..4170
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4200..4234
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4265..4299
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4350..4384
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4412..4446
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4470..4504
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4534..4568
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4599..4633
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4684..4718
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4746..4780
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4804..4838
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4868..4902
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      4933..4967
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5018..5052
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5080..5114
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5138..5172
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5202..5236
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5267..5301
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5356..5390
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5418..5452
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5476..5510
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5540..5574
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5605..5639
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5690..5724
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5752..5786
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5810..5844
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5874..5908
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      5939..5973
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6024..6058
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6086..6120
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6144..6178
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6208..6242
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6273..6307
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6358..6392
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6420..6454
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6478..6512
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6542..6576
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6607..6641
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6696..6730
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6758..6792
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6816..6850
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6880..6914
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      6945..6979
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7030..7064
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7092..7126
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7150..7184
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7214..7248
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7279..7313
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7364..7398
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7426..7460
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7484..7518
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7548..7582
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7613..7647
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7698..7732
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7760..7794
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7818..7852
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7882..7916
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     regulatory      7947..7981
                     /regulatory_class="enhancer"
                     /label=LuxR binding footprint
                     /note="
                     LuxO footprint is from paper 
                     Urbanowski2004JournalofBacteriology"
     protein_bind    8031..8049
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8078..8096
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8127..8145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8159..8177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8201..8219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8247..8265
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8289..8307
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8337..8355
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8386..8404
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8428..8446
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8491..8509
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8538..8556
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8587..8605
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8619..8637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8661..8679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8707..8725
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8749..8767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8797..8815
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8846..8864
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8888..8906
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8951..8969
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8998..9016
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9047..9065
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9079..9097
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9121..9139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9167..9185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9209..9227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9257..9275
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9306..9324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9348..9366
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9411..9429
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9458..9476
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9507..9525
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9539..9557
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9581..9599
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9627..9645
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9669..9687
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9717..9735
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9766..9784
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9808..9826
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      9853..9981
                     /label=B0015
     misc_feature    join(9982..10001,1)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
       61 tcactcaaag gcggtaatct cgagtcccgt caagtcagcg taatgctctg ccagtgttac
      121 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
      181 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
      241 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
      301 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
      361 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc
      421 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
      481 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
      541 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
      601 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca
      661 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
      721 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
      781 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
      841 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
      901 atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg gctcataaca
      961 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     1021 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
     1081 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
     1141 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
     1201 ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca
     1261 ccttcttcac gaggcagacc tcagcgctag cggagtgtat actggcttac tatgttggca
     1321 ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg
     1381 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc
     1441 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga
     1501 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
     1561 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
     1621 acaggactat aaagatacca ggcgtttccc ctggcggctc cctcgtgcgc tctcctgttc
     1681 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc
     1741 ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc
     1801 gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga
     1861 catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa
     1921 gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag
     1981 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa
     2041 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga
     2101 tcatcttatt aaggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2401 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattccat ggtgccacct
     2521 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2581 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
     2641 ccgcttctag aggctcatac tgcgtaagag tctttaacat aagcacctgt aggatcgtac
     2701 aggtttacgc taggctcctt tgtcaaaagt gatcgctaac ataagcacct gtaggatcgt
     2761 acaggtttac gccttcacgg gataatgtgt tctacaacat aagcacctgt aggatcgtac
     2821 aggtttacgc gcttaagtca atccaatgcg ttcaggtgca acataagcac ctgtaggatc
     2881 gtacaggttt acgcaactcc gtcatcgtgg atgtcaatat tggcaacata agcacctgta
     2941 ggatcgtaca ggtttacgct tgatttcacc gctggcgtca atgagtgctc atactgcgta
     3001 agagtcttta acataagcac ctgtaggatc gtacaggttt acgctaggct cctttgtcaa
     3061 aagtgatcgc taacataagc acctgtagga tcgtacaggt ttacgccttc acgggataat
     3121 gtgttctaca acataagcac ctgtaggatc gtacaggttt acgcgcttaa gtcaatccaa
     3181 tgcgttcagg tgcaacataa gcacctgtag gatcgtacag gtttacgcaa ctccgtcatc
     3241 gtggatgtca atattggcaa cataagcacc tgtaggatcg tacaggttta cgcttgattt
     3301 caccgctggc gtcaatttcc gctcatactg cgtaagagtc tttaacataa gcacctgtag
     3361 gatcgtacag gtttacgcta ggctcctttg tcaaaagtga tcgctaacat aagcacctgt
     3421 aggatcgtac aggtttacgc cttcacggga taatgtgttc tacaacataa gcacctgtag
     3481 gatcgtacag gtttacgcgc ttaagtcaat ccaatgcgtt caggtgcaac ataagcacct
     3541 gtaggatcgt acaggtttac gcaactccgt catcgtggat gtcaatattg gcaacataag
     3601 cacctgtagg atcgtacagg tttacgcttg atttcaccgc tggcgtcaat gactgctcat
     3661 actgcgtaag agtctttaac ataagcacct gtaggatcgt acaggtttac gctaggctcc
     3721 tttgtcaaaa gtgatcgcta acataagcac ctgtaggatc gtacaggttt acgccttcac
     3781 gggataatgt gttctacaac ataagcacct gtaggatcgt acaggtttac gcgcttaagt
     3841 caatccaatg cgttcaggtg caacataagc acctgtagga tcgtacaggt ttacgcaact
     3901 ccgtcatcgt ggatgtcaat attggcaaca taagcacctg taggatcgta caggtttacg
     3961 cttgatttca ccgctggcgt caattactag aggctcatac tgcgtaagag tctttaacat
     4021 aagcacctgt aggatcgtac aggtttacgc taggctcctt tgtcaaaagt gatcgctaac
     4081 ataagcacct gtaggatcgt acaggtttac gccttcacgg gataatgtgt tctacaacat
     4141 aagcacctgt aggatcgtac aggtttacgc gcttaagtca atccaatgcg ttcaggtgca
     4201 acataagcac ctgtaggatc gtacaggttt acgcaactcc gtcatcgtgg atgtcaatat
     4261 tggcaacata agcacctgta ggatcgtaca ggtttacgct tgatttcacc gctggcgtca
     4321 atgagtgctc atactgcgta agagtcttta acataagcac ctgtaggatc gtacaggttt
     4381 acgctaggct cctttgtcaa aagtgatcgc taacataagc acctgtagga tcgtacaggt
     4441 ttacgccttc acgggataat gtgttctaca acataagcac ctgtaggatc gtacaggttt
     4501 acgcgcttaa gtcaatccaa tgcgttcagg tgcaacataa gcacctgtag gatcgtacag
     4561 gtttacgcaa ctccgtcatc gtggatgtca atattggcaa cataagcacc tgtaggatcg
     4621 tacaggttta cgcttgattt caccgctggc gtcaatttcc gctcatactg cgtaagagtc
     4681 tttaacataa gcacctgtag gatcgtacag gtttacgcta ggctcctttg tcaaaagtga
     4741 tcgctaacat aagcacctgt aggatcgtac aggtttacgc cttcacggga taatgtgttc
     4801 tacaacataa gcacctgtag gatcgtacag gtttacgcgc ttaagtcaat ccaatgcgtt
     4861 caggtgcaac ataagcacct gtaggatcgt acaggtttac gcaactccgt catcgtggat
     4921 gtcaatattg gcaacataag cacctgtagg atcgtacagg tttacgcttg atttcaccgc
     4981 tggcgtcaat gactgctcat actgcgtaag agtctttaac ataagcacct gtaggatcgt
     5041 acaggtttac gctaggctcc tttgtcaaaa gtgatcgcta acataagcac ctgtaggatc
     5101 gtacaggttt acgccttcac gggataatgt gttctacaac ataagcacct gtaggatcgt
     5161 acaggtttac gcgcttaagt caatccaatg cgttcaggtg caacataagc acctgtagga
     5221 tcgtacaggt ttacgcaact ccgtcatcgt ggatgtcaat attggcaaca taagcacctg
     5281 taggatcgta caggtttacg cttgatttca ccgctggcgt caattactag aggctcatac
     5341 tgcgtaagag tctttaacat aagcacctgt aggatcgtac aggtttacgc taggctcctt
     5401 tgtcaaaagt gatcgctaac ataagcacct gtaggatcgt acaggtttac gccttcacgg
     5461 gataatgtgt tctacaacat aagcacctgt aggatcgtac aggtttacgc gcttaagtca
     5521 atccaatgcg ttcaggtgca acataagcac ctgtaggatc gtacaggttt acgcaactcc
     5581 gtcatcgtgg atgtcaatat tggcaacata agcacctgta ggatcgtaca ggtttacgct
     5641 tgatttcacc gctggcgtca atgagtgctc atactgcgta agagtcttta acataagcac
     5701 ctgtaggatc gtacaggttt acgctaggct cctttgtcaa aagtgatcgc taacataagc
     5761 acctgtagga tcgtacaggt ttacgccttc acgggataat gtgttctaca acataagcac
     5821 ctgtaggatc gtacaggttt acgcgcttaa gtcaatccaa tgcgttcagg tgcaacataa
     5881 gcacctgtag gatcgtacag gtttacgcaa ctccgtcatc gtggatgtca atattggcaa
     5941 cataagcacc tgtaggatcg tacaggttta cgcttgattt caccgctggc gtcaatttcc
     6001 gctcatactg cgtaagagtc tttaacataa gcacctgtag gatcgtacag gtttacgcta
     6061 ggctcctttg tcaaaagtga tcgctaacat aagcacctgt aggatcgtac aggtttacgc
     6121 cttcacggga taatgtgttc tacaacataa gcacctgtag gatcgtacag gtttacgcgc
     6181 ttaagtcaat ccaatgcgtt caggtgcaac ataagcacct gtaggatcgt acaggtttac
     6241 gcaactccgt catcgtggat gtcaatattg gcaacataag cacctgtagg atcgtacagg
     6301 tttacgcttg atttcaccgc tggcgtcaat gactgctcat actgcgtaag agtctttaac
     6361 ataagcacct gtaggatcgt acaggtttac gctaggctcc tttgtcaaaa gtgatcgcta
     6421 acataagcac ctgtaggatc gtacaggttt acgccttcac gggataatgt gttctacaac
     6481 ataagcacct gtaggatcgt acaggtttac gcgcttaagt caatccaatg cgttcaggtg
     6541 caacataagc acctgtagga tcgtacaggt ttacgcaact ccgtcatcgt ggatgtcaat
     6601 attggcaaca taagcacctg taggatcgta caggtttacg cttgatttca ccgctggcgt
     6661 caattactag aggctcatac tgcgtaagag tctttaacat aagcacctgt aggatcgtac
     6721 aggtttacgc taggctcctt tgtcaaaagt gatcgctaac ataagcacct gtaggatcgt
     6781 acaggtttac gccttcacgg gataatgtgt tctacaacat aagcacctgt aggatcgtac
     6841 aggtttacgc gcttaagtca atccaatgcg ttcaggtgca acataagcac ctgtaggatc
     6901 gtacaggttt acgcaactcc gtcatcgtgg atgtcaatat tggcaacata agcacctgta
     6961 ggatcgtaca ggtttacgct tgatttcacc gctggcgtca atgagtgctc atactgcgta
     7021 agagtcttta acataagcac ctgtaggatc gtacaggttt acgctaggct cctttgtcaa
     7081 aagtgatcgc taacataagc acctgtagga tcgtacaggt ttacgccttc acgggataat
     7141 gtgttctaca acataagcac ctgtaggatc gtacaggttt acgcgcttaa gtcaatccaa
     7201 tgcgttcagg tgcaacataa gcacctgtag gatcgtacag gtttacgcaa ctccgtcatc
     7261 gtggatgtca atattggcaa cataagcacc tgtaggatcg tacaggttta cgcttgattt
     7321 caccgctggc gtcaatttcc gctcatactg cgtaagagtc tttaacataa gcacctgtag
     7381 gatcgtacag gtttacgcta ggctcctttg tcaaaagtga tcgctaacat aagcacctgt
     7441 aggatcgtac aggtttacgc cttcacggga taatgtgttc tacaacataa gcacctgtag
     7501 gatcgtacag gtttacgcgc ttaagtcaat ccaatgcgtt caggtgcaac ataagcacct
     7561 gtaggatcgt acaggtttac gcaactccgt catcgtggat gtcaatattg gcaacataag
     7621 cacctgtagg atcgtacagg tttacgcttg atttcaccgc tggcgtcaat gactgctcat
     7681 actgcgtaag agtctttaac ataagcacct gtaggatcgt acaggtttac gctaggctcc
     7741 tttgtcaaaa gtgatcgcta acataagcac ctgtaggatc gtacaggttt acgccttcac
     7801 gggataatgt gttctacaac ataagcacct gtaggatcgt acaggtttac gcgcttaagt
     7861 caatccaatg cgttcaggtg caacataagc acctgtagga tcgtacaggt ttacgcaact
     7921 ccgtcatcgt ggatgtcaat attggcaaca taagcacctg taggatcgta caggtttacg
     7981 cttgatttca ccgctggcgt caattactag agatcgattt tagagctgcc tccctatcag
     8041 tgatagagat tgctacaact gcaaggtagt cccggtatcc ctatcagtga tagagactga
     8101 tagctacgag tctgagatcc tacttgtccc tatcagtgat agagacagta ccgagttttc
     8161 cctatcagtg atagagagct catactgcga cagagtcttt tccctatcag tgatagagat
     8221 aggctccttt gtcaaaagtg atcgcttccc tatcagtgat agagacttca cgggataatg
     8281 tgttctactc cctatcagtg atagagagct taagtcaatc caatgcgttc aggtgctccc
     8341 tatcagtgat agagaaactc cgtcatcgtg gatgtcaata ctgactccct atcagtgata
     8401 gagattgatt tcaccgctgg cgtcaattcc ctatcagtga tagagactta ggtgtcccta
     8461 gaattactag agatcgattt tagagctgcc tccctatcag tgatagagat tgctacaact
     8521 gcaaggtagt cccggtatcc ctatcagtga tagagactga tagctacgag tctgagatcc
     8581 tacttgtccc tatcagtgat agagacagta ccgagttttc cctatcagtg atagagagct
     8641 catactgcga cagagtcttt tccctatcag tgatagagat aggctccttt gtcaaaagtg
     8701 atcgcttccc tatcagtgat agagacttca cgggataatg tgttctactc cctatcagtg
     8761 atagagagct taagtcaatc caatgcgttc aggtgctccc tatcagtgat agagaaactc
     8821 cgtcatcgtg gatgtcaata ctgactccct atcagtgata gagattgatt tcaccgctgg
     8881 cgtcaattcc ctatcagtga tagagactta ggtgtcccta gaattactag agatcgattt
     8941 tagagctgcc tccctatcag tgatagagat tgctacaact gcaaggtagt cccggtatcc
     9001 ctatcagtga tagagactga tagctacgag tctgagatcc tacttgtccc tatcagtgat
     9061 agagacagta ccgagttttc cctatcagtg atagagagct catactgcga cagagtcttt
     9121 tccctatcag tgatagagat aggctccttt gtcaaaagtg atcgcttccc tatcagtgat
     9181 agagacttca cgggataatg tgttctactc cctatcagtg atagagagct taagtcaatc
     9241 caatgcgttc aggtgctccc tatcagtgat agagaaactc cgtcatcgtg gatgtcaata
     9301 ctgactccct atcagtgata gagattgatt tcaccgctgg cgtcaattcc ctatcagtga
     9361 tagagactta ggtgtcccta gaattactag agatcgattt tagagctgcc tccctatcag
     9421 tgatagagat tgctacaact gcaaggtagt cccggtatcc ctatcagtga tagagactga
     9481 tagctacgag tctgagatcc tacttgtccc tatcagtgat agagacagta ccgagttttc
     9541 cctatcagtg atagagagct catactgcga cagagtcttt tccctatcag tgatagagat
     9601 aggctccttt gtcaaaagtg atcgcttccc tatcagtgat agagacttca cgggataatg
     9661 tgttctactc cctatcagtg atagagagct taagtcaatc caatgcgttc aggtgctccc
     9721 tatcagtgat agagaaactc cgtcatcgtg gatgtcaata ctgactccct atcagtgata
     9781 gagattgatt tcaccgctgg cgtcaattcc ctatcagtga tagagactta ggtgtcccta
     9841 gaattactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     9901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     9961 gtgggccttt ctgcgtttat atactagtag cggccgctgc a
//
