LOCUS       pLNL1126                4247 bp ds-DNA     circular     19-FEB-2017
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  . .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(1046..1193)
                     /label=KanR Promoter
                     /ApEinfo_fwdcolor="#93ff35"
                     /ApEinfo_revcolor="#93ff35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(230..1045)
                     /label=KanR
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(203..229)
                     /label=KanR Terminator
                     /ApEinfo_fwdcolor="#93ff35"
                     /ApEinfo_revcolor="#93ff35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(144..149,151..154)
                     /label=BsmBI
                     /ApEinfo_fwdcolor="#00ff15"
                     /ApEinfo_revcolor="#e72e00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(join(2062..2065,2067..2072))
                     /label=BsmBI(1)
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#00ff15"
                     /ApEinfo_revcolor="#e72e00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3468..4175
                     /label=mRuby2
                     /ApEinfo_fwdcolor="#FF454E"
                     /ApEinfo_revcolor="#FF454E"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1194..2034
                     /label=p15A
                     /ApEinfo_fwdcolor="#FAFFB7"
                     /ApEinfo_revcolor="#FAFFB7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2292..3461
                     /label=FaAAT2
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4189..4243
                     /label=tL3S2P24
                     /ApEinfo_fwdcolor="#b0b0ff"
                     /ApEinfo_revcolor="#b0b0ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2268..2270
                     /label=BBa_B0029
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2227..2261
                     /label=J23110
                     /ApEinfo_fwdcolor="#48a4ff"
                     /ApEinfo_revcolor="#48a4ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2286..2287
                     /label=BBa_B0029(1)
                     /ApEinfo_label="BBa_B0029"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gaaatctgct cgtcagtggt gctcacactg acgaatcatg tacagatcat accgatgact
       61 gcctggcgac tcacaactaa gcaagacagc cggaaccagc gccggcgaac accactgcat
      121 atatggcata tcacaacagt ccacgtctca agcagttaca gagatgttac gaaccactag
      181 tgcactgcag tacagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact
      241 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
      301 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
      361 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
      421 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
      481 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
      541 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
      601 ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
      661 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
      721 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
      781 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
      841 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
      901 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
      961 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag
     1021 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1081 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     1141 acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtagcgga
     1201 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
     1261 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
     1321 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
     1381 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
     1441 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     1501 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1561 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     1621 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     1681 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
     1741 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
     1801 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
     1861 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
     1921 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac
     1981 gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttgcggcc
     2041 gcccctgaat tcgcatctag actgatgaga cgtggtagag ccacaaacag ccggtacaag
     2101 caacgatctc caggaccatc tgaatcatgc gcggatgaca cgaactcacg acggcgatca
     2161 cagacattaa cccacagtac agacactgcg acaacgtggc aattcgtcgc aatacaacgg
     2221 atttacttta cggctagctc agtcctaggt acaatgctag cgctagcttc nnnnNNNNNN
     2281 NNNNNtctat gtcttataag aataaccatt ctattttatc aaaaccaaat gatccagtcg
     2341 aagtaattag agatgcatta tctaaggctt tacagttcta ctacccattg gccggtagat
     2401 tgagagaagg tccaaataag aagttgatgg ttgactgtac cggagaaggt attttatttg
     2461 tagaggctaa tgccgaagtt accttagatg agttaggtga tgctatctta ccaccatgcc
     2521 cattcttaga tggtttctta tttaacgttc caggttcaga tggtatttta ggttctccat
     2581 tgtgtttgat acaagtaact agattgtcat gtggtggatt catattcgca ttaagattga
     2641 accatactat ctgcgatgct ttgggtttag ttcagttctt gaacgctgta ggtgaaatag
     2701 ctcaaggtaa gtacgcacca tctataaccc ctgtttggga aagagagtta ttatcagcaa
     2761 gagatcctcc aagaatctca tgcacacatg aggaatttga cgattctatt gatcactctt
     2821 acccaaatta tggagctact gttcagcagt gttactgttt tggtccaaag gagatcaaat
     2881 ctttgagaga acatttgcca ccacatttgt ctacatgttc ttctactttt gaattgatca
     2941 ccgcctgcgt ttggaagtgc agaactatat cattggatat ggacccagaa cagatagtaa
     3001 gattgtcttg tgtagtaacc gccttaggta agcataacaa cgtttgcttg ccattgggtt
     3061 attacggtaa tactttcacc tatccagcag tcgtttctac cgccgagagg ttgtgtaact
     3121 cacctttagg ttacgcagta gaattggtca agaaatctaa ggctaaaatg tctgaagagt
     3181 atttaagatc agccatagat tttgttgagg tcaggggaag acctccattc gccttagaag
     3241 gtatgtcaga tttcttagtt tctgataata caagaactgg tttgggtgaa atagactttg
     3301 gttttggtaa accagtttac gctggtgttg ctaaatcaac cgatttaatc tcattttatg
     3361 taaggtcaac aaataaggag gaaagagaaa tcttagtccc tgtttgtttg ccaatattgt
     3421 caatggagat atttcaacaa gagttaaaga agatgatcgg aggatccgtg tccaaaggag
     3481 aggagttaat caaggaaaac atgagaatga aagttgtcat ggagggctcc gttaatggtc
     3541 accaattcaa gtgtacaggg gaaggtgaag gtaatcctta catgggtaca caaactatga
     3601 gaattaaagt aattgaaggc ggaccactac catttgcatt tgacattctg gcaacgtcat
     3661 tcatgtacgg atcacgaact ttcatcaagt accctaaagg tataccagac tttttcaagc
     3721 aatcttttcc agagggtttt acatgggaaa gggttacaag atacgaagat gggggtgtcg
     3781 tcacagttat gcaagatact tcattagaag atggctgcct tgtctatcat gtgcaagtaa
     3841 gaggggtgaa ttttccttct aacggacctg tgatgcagaa aaagaccaaa ggttgggaac
     3901 caaatactga aatgatgtac ccagctgatg gaggtttgag aggctacaca cacatggcgc
     3961 ttaaagttga tggtggaggt catttgtctt gtagttttgt taccacttat cgttctaaaa
     4021 agactgttgg caatatcaaa atgccaggaa tacatgctgt agaccacaga ctagaaagac
     4081 tcgaagagag cgataacgaa atgttcgttg tacagagaga gcatgccgta gccaaatttg
     4141 ctggcttagg cggtggtatg gatgaattgt ataagtaact cgagtggcct cggtaccaaa
     4201 ttccagaaaa gacacccgaa agggtgtttt ttcgttttgg tccgctg 
//
