LOCUS       pLNL1127                4445 bp ds-DNA     circular     19-FEB-2017
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  . .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(1046..1193)
                     /label=KanR Promoter
                     /ApEinfo_fwdcolor="#93ff35"
                     /ApEinfo_revcolor="#93ff35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(230..1045)
                     /label=KanR
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(203..229)
                     /label=KanR Terminator
                     /ApEinfo_fwdcolor="#93ff35"
                     /ApEinfo_revcolor="#93ff35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(144..149,151..154)
                     /label=BsmBI
                     /ApEinfo_fwdcolor="#00ff15"
                     /ApEinfo_revcolor="#e72e00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(join(2062..2065,2067..2072))
                     /label=BsmBI(1)
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#00ff15"
                     /ApEinfo_revcolor="#e72e00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3666..4373
                     /label=mRuby2
                     /ApEinfo_fwdcolor="#FF454E"
                     /ApEinfo_revcolor="#FF454E"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1194..2034
                     /label=p15A
                     /ApEinfo_fwdcolor="#FAFFB7"
                     /ApEinfo_revcolor="#FAFFB7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2292..3659
                     /label=RhAAT1
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4387..4441
                     /label=tL3S2P24
                     /ApEinfo_fwdcolor="#b0b0ff"
                     /ApEinfo_revcolor="#b0b0ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2268..2270
                     /label=BBa_B0029
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2227..2261
                     /label=J23110
                     /ApEinfo_fwdcolor="#48a4ff"
                     /ApEinfo_revcolor="#48a4ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2286..2287
                     /label=BBa_B0029(1)
                     /ApEinfo_label="BBa_B0029"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gaaatctgct cgtcagtggt gctcacactg acgaatcatg tacagatcat accgatgact
       61 gcctggcgac tcacaactaa gcaagacagc cggaaccagc gccggcgaac accactgcat
      121 atatggcata tcacaacagt ccacgtctca agcagttaca gagatgttac gaaccactag
      181 tgcactgcag tacagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact
      241 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
      301 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
      361 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
      421 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
      481 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
      541 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
      601 ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
      661 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
      721 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
      781 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
      841 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
      901 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
      961 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag
     1021 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1081 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     1141 acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtagcgga
     1201 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
     1261 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
     1321 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
     1381 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
     1441 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     1501 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1561 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     1621 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     1681 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
     1741 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
     1801 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
     1861 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
     1921 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac
     1981 gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttgcggcc
     2041 gcccctgaat tcgcatctag actgatgaga cgtggtagag ccacaaacag ccggtacaag
     2101 caacgatctc caggaccatc tgaatcatgc gcggatgaca cgaactcacg acggcgatca
     2161 cagacattaa cccacagtac agacactgcg acaacgtggc aattcgtcgc aatacaacgg
     2221 atttacttta cggctagctc agtcctaggt acaatgctag cgctagcttc nnnnNNNNNN
     2281 NNNNNtctat ggagaagatc gaagtctcta taatttcaag agacacaata aaaccttctg
     2341 ctgcctcatc ttctttacat ccttacaagt tgtcaataat tgatcaattc acacctacaa
     2401 cttattttcc tgtcatattc ttttacccaa ttactgacag agtctttaat ttgccacaga
     2461 cattaaccga cttaaagaat accgtatcac aggctttaac tttatatcat ccattatctg
     2521 gaaggatcaa gaacaattta tacattgatg atttcgaagc tggaatccct tacttagaag
     2581 ccagagtcaa ttttcatatg atagatttct taagattgcc taaaattgaa tggttaaatg
     2641 agtttgttcc tatggctcct tacaggaaag aaactatttc agagttcttg ccattgttgg
     2701 gtattcaagt aaacatattt gattcaggta tcgctattgg agtatcattt tcacacaaaa
     2761 ttaatgacgg acaaaccgca tcatgtttct taaagtcatg ggttgcaata tttaggggtt
     2821 acagaaacaa gattatacat ccaaatttgt cacaagctgc tttattgttg ccatcaaggg
     2881 atgatttgcc agaaaagtat gtagccatga tggagagaat gtggttcggt gaaaagaagg
     2941 tcgtcactag aagatttgtt ttcgatgcta aagcaatatc agcattacaa gatgaaggaa
     3001 agtcagaata cgtccctaaa ccttctaggg tacaagcatt gacaggattc ttatggaagc
     3061 atcaattggc agcctcaaga gcattgtctt ctggaacttc tactaggttt tctgttgcct
     3121 ctcagactgt caatttaaga tcaaagatga atatgaagac tactttagat aacgctattg
     3181 gtaatatttt cttgtgggcc tcagccagat tggacttgaa tgacactgcc ccaggatcat
     3241 ctgacttgaa gttgtgtgat ttggtaaatt tgttgaatga gtctatcaaa gaatttaatt
     3301 cagactattt ggaaattttg aaaggaaagg aaggttacgg aggtatgtgt gatttattgg
     3361 atttcatgga ggagggttct tttgttgagc cagctccaga attttattca ttctcttcat
     3421 ggaccagatt cttcgatcaa gtcgactttg gttggggtag gccatcttgg gtaggtttct
     3481 ctggtagagt tgaaactaga aattttacta tatttgttga aacccaatgt gatgatggaa
     3541 tcgatgcttg ggttaccgtc gacgaaaagc aaatggctat gttagaacaa gacccacagt
     3601 tcttggcttt tgcttcacct aatcctagaa tatctattgc atcatctgtt ggtatggacg
     3661 gatccgtgtc caaaggagag gagttaatca aggaaaacat gagaatgaaa gttgtcatgg
     3721 agggctccgt taatggtcac caattcaagt gtacagggga aggtgaaggt aatccttaca
     3781 tgggtacaca aactatgaga attaaagtaa ttgaaggcgg accactacca tttgcatttg
     3841 acattctggc aacgtcattc atgtacggat cacgaacttt catcaagtac cctaaaggta
     3901 taccagactt tttcaagcaa tcttttccag agggttttac atgggaaagg gttacaagat
     3961 acgaagatgg gggtgtcgtc acagttatgc aagatacttc attagaagat ggctgccttg
     4021 tctatcatgt gcaagtaaga ggggtgaatt ttccttctaa cggacctgtg atgcagaaaa
     4081 agaccaaagg ttgggaacca aatactgaaa tgatgtaccc agctgatgga ggtttgagag
     4141 gctacacaca catggcgctt aaagttgatg gtggaggtca tttgtcttgt agttttgtta
     4201 ccacttatcg ttctaaaaag actgttggca atatcaaaat gccaggaata catgctgtag
     4261 accacagact agaaagactc gaagagagcg ataacgaaat gttcgttgta cagagagagc
     4321 atgccgtagc caaatttgct ggcttaggcg gtggtatgga tgaattgtat aagtaactcg
     4381 agtggcctcg gtaccaaatt ccagaaaaga cacccgaaag ggtgtttttt cgttttggtc
     4441 cgctg     
//
