LOCUS       pLNL1133                4442 bp ds-DNA     circular     19-FEB-2017
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  . .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(1046..1193)
                     /label=KanR Promoter
                     /ApEinfo_fwdcolor="#93ff35"
                     /ApEinfo_revcolor="#93ff35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(230..1045)
                     /label=KanR
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(203..229)
                     /label=KanR Terminator
                     /ApEinfo_fwdcolor="#93ff35"
                     /ApEinfo_revcolor="#93ff35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(144..149,151..154)
                     /label=BsmBI
                     /ApEinfo_fwdcolor="#00ff15"
                     /ApEinfo_revcolor="#e72e00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(join(2062..2065,2067..2072))
                     /label=BsmBI(1)
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#00ff15"
                     /ApEinfo_revcolor="#e72e00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3663..4370
                     /label=mRuby2
                     /ApEinfo_fwdcolor="#FF454E"
                     /ApEinfo_revcolor="#FF454E"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1194..2034
                     /label=p15A
                     /ApEinfo_fwdcolor="#FAFFB7"
                     /ApEinfo_revcolor="#FAFFB7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2292..3656
                     /label=ScEEB1
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4384..4438
                     /label=tL3S2P24
                     /ApEinfo_fwdcolor="#b0b0ff"
                     /ApEinfo_revcolor="#b0b0ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2268..2270
                     /label=BBa_B0029
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2227..2261
                     /label=J23110
                     /ApEinfo_fwdcolor="#48a4ff"
                     /ApEinfo_revcolor="#48a4ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2286..2287
                     /label=BBa_B0029(1)
                     /ApEinfo_label="BBa_B0029"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gaaatctgct cgtcagtggt gctcacactg acgaatcatg tacagatcat accgatgact
       61 gcctggcgac tcacaactaa gcaagacagc cggaaccagc gccggcgaac accactgcat
      121 atatggcata tcacaacagt ccacgtctca agcagttaca gagatgttac gaaccactag
      181 tgcactgcag tacagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact
      241 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
      301 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
      361 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
      421 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
      481 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
      541 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
      601 ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
      661 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
      721 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
      781 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
      841 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
      901 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
      961 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag
     1021 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1081 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     1141 acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtagcgga
     1201 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
     1261 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
     1321 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
     1381 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
     1441 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     1501 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1561 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     1621 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     1681 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
     1741 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
     1801 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
     1861 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
     1921 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac
     1981 gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttgcggcc
     2041 gcccctgaat tcgcatctag actgatgaga cgtggtagag ccacaaacag ccggtacaag
     2101 caacgatctc caggaccatc tgaatcatgc gcggatgaca cgaactcacg acggcgatca
     2161 cagacattaa cccacagtac agacactgcg acaacgtggc aattcgtcgc aatacaacgg
     2221 atttacttta cggctagctc agtcctaggt acaatgctag cgctagcttc nnnnNNNNNN
     2281 NNNNNtctat gtttcgctcg ggttactatc caactgtcac tcctagccat tggggctata
     2341 acggcaccgt caaacatgta ctgggagaga aaggaaccaa gtctttggct ttcagagatt
     2401 ctaagcgcca aattcctctt catgaattcg taaccaaaca cgtgcctaca ctcaaagatg
     2461 gtgcaaattt taggttaaac agcttgcttt tcacaggtta cttacagacc ctttatttgt
     2521 cagccggtga tttttccaaa aaatttcagg tattttatgg aagagagatt ataaaatttt
     2581 ctgatggagg ggtctgcact gcagattggg ttatgcctga atgggagcaa acatattcat
     2641 taaatgctga aaaggctagt tttaacgaga aacaattttc gaacgatgaa aaggccaccc
     2701 atccaaaggg ttggcctcgt cttcacccga gaactagata tctatcctct gaggaacttg
     2761 aaaaatgtca ttctaagggt tattcttacc ctttagtggt agtacttcat ggtcttgcag
     2821 ggggaagcca tgagcctctc atcagggcat tatctgaaga tctatcaaaa gtcggtgacg
     2881 gtaaattcca agtggtagtc cttaatgcta gaggttgctc aagatctaaa gtcaccaccc
     2941 gtagaatctt taccgcatta cacacaggtg atgtgagaga atttttgaac caccaaaaag
     3001 ctctatttcc gcaaagaaaa atatatgcag tcggaacatc atttggagca gccatgctaa
     3061 cgaactactt aggtgaagaa ggagataact gtccattaaa tgctgcagtc gctctctcaa
     3121 acccatggga tttcgtacac acttgggaca agttggctca tgattggtgg tccaaccata
     3181 ttttttctag aactttaaca caatttctaa caaggactgt caaggttaat atgaatgagt
     3241 tgcaggtacc agagaatttt gaggtttcgc acaaaccgac agttgagaaa ccagtctttt
     3301 atacgtatac cagagaaaat ttggaaaagg ctgaaaaatt tacagacata ttagaattcg
     3361 ataatctttt cactgcccca tcgatgggcc tgccggacgg gttaacatat tatagaaagg
     3421 ccagttcgat aaatagattg cctaatatca aaatcccaac tttaataatc aacgcaacgg
     3481 atgatccagt tacaggtgaa aacgtaattc cgtacaaaca agctagagaa aatccttgtg
     3541 tgttgttatg cgagactgac ttgggtggac atttggctta tctcgacaat gaaagtaatt
     3601 cctggttaac aaagcaagct gccgagttct tgggcagctt tgatgagtta gttttaggat
     3661 ccgtgtccaa aggagaggag ttaatcaagg aaaacatgag aatgaaagtt gtcatggagg
     3721 gctccgttaa tggtcaccaa ttcaagtgta caggggaagg tgaaggtaat ccttacatgg
     3781 gtacacaaac tatgagaatt aaagtaattg aaggcggacc actaccattt gcatttgaca
     3841 ttctggcaac gtcattcatg tacggatcac gaactttcat caagtaccct aaaggtatac
     3901 cagacttttt caagcaatct tttccagagg gttttacatg ggaaagggtt acaagatacg
     3961 aagatggggg tgtcgtcaca gttatgcaag atacttcatt agaagatggc tgccttgtct
     4021 atcatgtgca agtaagaggg gtgaattttc cttctaacgg acctgtgatg cagaaaaaga
     4081 ccaaaggttg ggaaccaaat actgaaatga tgtacccagc tgatggaggt ttgagaggct
     4141 acacacacat ggcgcttaaa gttgatggtg gaggtcattt gtcttgtagt tttgttacca
     4201 cttatcgttc taaaaagact gttggcaata tcaaaatgcc aggaatacat gctgtagacc
     4261 acagactaga aagactcgaa gagagcgata acgaaatgtt cgttgtacag agagagcatg
     4321 ccgtagccaa atttgctggc ttaggcggtg gtatggatga attgtataag taactcgagt
     4381 ggcctcggta ccaaattcca gaaaagacac ccgaaagggt gttttttcgt tttggtccgc
     4441 tg     
//
