LOCUS       pLNL1134                4427 bp ds-DNA     circular     19-FEB-2017
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  . .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(1046..1193)
                     /label=KanR Promoter
                     /ApEinfo_fwdcolor="#93ff35"
                     /ApEinfo_revcolor="#93ff35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(230..1045)
                     /label=KanR
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(203..229)
                     /label=KanR Terminator
                     /ApEinfo_fwdcolor="#93ff35"
                     /ApEinfo_revcolor="#93ff35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(144..149,151..154)
                     /label=BsmBI
                     /ApEinfo_fwdcolor="#00ff15"
                     /ApEinfo_revcolor="#e72e00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(join(2062..2065,2067..2072))
                     /label=BsmBI(1)
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#00ff15"
                     /ApEinfo_revcolor="#e72e00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3648..4355
                     /label=mRuby2
                     /ApEinfo_fwdcolor="#FF454E"
                     /ApEinfo_revcolor="#FF454E"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1194..2034
                     /label=p15A
                     /ApEinfo_fwdcolor="#FAFFB7"
                     /ApEinfo_revcolor="#FAFFB7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2292..3641
                     /label=ScEHT1
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4369..4423
                     /label=tL3S2P24
                     /ApEinfo_fwdcolor="#b0b0ff"
                     /ApEinfo_revcolor="#b0b0ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2268..2270
                     /label=BBa_B0029
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2227..2261
                     /label=J23110
                     /ApEinfo_fwdcolor="#48a4ff"
                     /ApEinfo_revcolor="#48a4ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2286..2287
                     /label=BBa_B0029(1)
                     /ApEinfo_label="BBa_B0029"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gaaatctgct cgtcagtggt gctcacactg acgaatcatg tacagatcat accgatgact
       61 gcctggcgac tcacaactaa gcaagacagc cggaaccagc gccggcgaac accactgcat
      121 atatggcata tcacaacagt ccacgtctca agcagttaca gagatgttac gaaccactag
      181 tgcactgcag tacagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact
      241 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
      301 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
      361 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
      421 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
      481 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
      541 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
      601 ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
      661 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
      721 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
      781 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
      841 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
      901 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
      961 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag
     1021 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1081 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     1141 acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtagcgga
     1201 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
     1261 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
     1321 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
     1381 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
     1441 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     1501 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1561 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     1621 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     1681 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
     1741 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
     1801 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
     1861 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
     1921 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac
     1981 gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttgcggcc
     2041 gcccctgaat tcgcatctag actgatgaga cgtggtagag ccacaaacag ccggtacaag
     2101 caacgatctc caggaccatc tgaatcatgc gcggatgaca cgaactcacg acggcgatca
     2161 cagacattaa cccacagtac agacactgcg acaacgtggc aattcgtcgc aatacaacgg
     2221 atttacttta cggctagctc agtcctaggt acaatgctag cgctagcttc nnnnNNNNNN
     2281 NNNNNtctat gtcagaagtt tccaaatggc cagcaatcaa cccattccat tggggataca
     2341 atggtacagt ttcgcatatt gtcggtgaaa atggttccat taaactccat ttaaaagaca
     2401 acaaggagca agttgatttt gacgagttcg ctaacaaata tgtcccaacg ttgaagaatg
     2461 gtgcccaatt caaattgagt ccttacttgt tcacaggtat tttgcaaact ttgtacttag
     2521 gtgctgctga tttctctaag aaatttcctg tattctacgg cagggaaatt gtcaaattct
     2581 cggatggtgg agtttgcacc gctgactggc tcatagattc atggaaaaag gattatgaat
     2641 tcgatcaaag tactacgagc tttgataaaa aaaaatttga taaagacgag aaggcgacac
     2701 atccagaagg atggcctcgt ttacaaccac gtacaaggta cctgaaagat aatgagttgg
     2761 aagaactacg ggaggttgat ctacccctag tagttattct acatggtctt gctggtggta
     2821 gtcatgagcc gattataaga tctcttgctg aaaacctgtc tcgcagtggg agatttcaag
     2881 tggtcgtcct aaataccaga ggttgtgcac gttccaaaat taccaccaga aatttattta
     2941 cagcttatca cacaatggat attcgcgagt ttttgcaaag agaaaagcaa agacatccag
     3001 atagaaaact atacgctgtg ggatgctctt ttggtgctac gatgctggca aactatctgg
     3061 gagaagaggg cgataaatca cctttatccg cagctgctac tttgtgcaat ccttgggatc
     3121 ttctcctttc agcaattagg atgagccagg attggtggtc aagaacttta ttttccaaaa
     3181 atattgcgca attcttaaca agaaccgttc aggttaatat gggtgaatta ggagttccaa
     3241 atggctctct ccccgatcat cctcccacag tcaagaatcc atctttctat atgttcacgc
     3301 ctgaaaatct aataaaggca aagagcttta aatcgacccg ggaatttgat gaagtgtaca
     3361 ctgcgcctgc tttaggcttc ccaaatgcta tggagtatta taaagcggcc agctcaataa
     3421 acagagttga tacaattcgg gttcctaccc ttgttatcaa ttccagggat gatcctgttg
     3481 tcggcccaga tcaaccatac tcaatcgtgg aaaagaatcc tcgtattttg tattgtagaa
     3541 ccgatttagg tggtcattta gcttacctag ataaagacaa caactcgtgg gctaccaagg
     3601 caattgcaga atttttcact aagtttgatg aattagtcgt aggatccgtg tccaaaggag
     3661 aggagttaat caaggaaaac atgagaatga aagttgtcat ggagggctcc gttaatggtc
     3721 accaattcaa gtgtacaggg gaaggtgaag gtaatcctta catgggtaca caaactatga
     3781 gaattaaagt aattgaaggc ggaccactac catttgcatt tgacattctg gcaacgtcat
     3841 tcatgtacgg atcacgaact ttcatcaagt accctaaagg tataccagac tttttcaagc
     3901 aatcttttcc agagggtttt acatgggaaa gggttacaag atacgaagat gggggtgtcg
     3961 tcacagttat gcaagatact tcattagaag atggctgcct tgtctatcat gtgcaagtaa
     4021 gaggggtgaa ttttccttct aacggacctg tgatgcagaa aaagaccaaa ggttgggaac
     4081 caaatactga aatgatgtac ccagctgatg gaggtttgag aggctacaca cacatggcgc
     4141 ttaaagttga tggtggaggt catttgtctt gtagttttgt taccacttat cgttctaaaa
     4201 agactgttgg caatatcaaa atgccaggaa tacatgctgt agaccacaga ctagaaagac
     4261 tcgaagagag cgataacgaa atgttcgttg tacagagaga gcatgccgta gccaaatttg
     4321 ctggcttagg cggtggtatg gatgaattgt ataagtaact cgagtggcct cggtaccaaa
     4381 ttccagaaaa gacacccgaa agggtgtttt ttcgttttgg tccgctg 
//
