LOCUS       pLNL1135                3737 bp ds-DNA     circular     19-FEB-2017
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  . .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(1046..1193)
                     /label=KanR Promoter
                     /ApEinfo_fwdcolor="#93ff35"
                     /ApEinfo_revcolor="#93ff35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(230..1045)
                     /label=KanR
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(203..229)
                     /label=KanR Terminator
                     /ApEinfo_fwdcolor="#93ff35"
                     /ApEinfo_revcolor="#93ff35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(144..149,151..154)
                     /label=BsmBI
                     /ApEinfo_fwdcolor="#00ff15"
                     /ApEinfo_revcolor="#e72e00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(join(2062..2065,2067..2072))
                     /label=BsmBI(1)
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#00ff15"
                     /ApEinfo_revcolor="#e72e00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2958..3665
                     /label=mRuby2
                     /ApEinfo_fwdcolor="#FF454E"
                     /ApEinfo_revcolor="#FF454E"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1194..2034
                     /label=p15A
                     /ApEinfo_fwdcolor="#FAFFB7"
                     /ApEinfo_revcolor="#FAFFB7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2295..2948
                     /label=CAT
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3679..3733
                     /label=tL3S2P24
                     /ApEinfo_fwdcolor="#b0b0ff"
                     /ApEinfo_revcolor="#b0b0ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2268..2270
                     /label=BBa_B0029
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2227..2261
                     /label=J23110
                     /ApEinfo_fwdcolor="#48a4ff"
                     /ApEinfo_revcolor="#48a4ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2286..2287
                     /label=BBa_B0029(1)
                     /ApEinfo_label="BBa_B0029"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gaaatctgct cgtcagtggt gctcacactg acgaatcatg tacagatcat accgatgact
       61 gcctggcgac tcacaactaa gcaagacagc cggaaccagc gccggcgaac accactgcat
      121 atatggcata tcacaacagt ccacgtctca agcagttaca gagatgttac gaaccactag
      181 tgcactgcag tacagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact
      241 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
      301 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
      361 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
      421 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
      481 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
      541 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
      601 ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
      661 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
      721 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
      781 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
      841 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
      901 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
      961 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag
     1021 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1081 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     1141 acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtagcgga
     1201 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
     1261 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
     1321 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
     1381 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
     1441 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     1501 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1561 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     1621 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     1681 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
     1741 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
     1801 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
     1861 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
     1921 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac
     1981 gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttgcggcc
     2041 gcccctgaat tcgcatctag actgatgaga cgtggtagag ccacaaacag ccggtacaag
     2101 caacgatctc caggaccatc tgaatcatgc gcggatgaca cgaactcacg acggcgatca
     2161 cagacattaa cccacagtac agacactgcg acaacgtggc aattcgtcgc aatacaacgg
     2221 atttacttta cggctagctc agtcctaggt acaatgctag cgctagcttc nnnnNNNNNN
     2281 NNNNNtctat gatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     2341 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     2401 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     2461 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa
     2521 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     2581 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     2641 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     2701 ggtttattga gaatatgttt ttcgtttcag ccaatccctg ggtgagtttc accagttttg
     2761 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     2821 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     2881 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     2941 gcggggcgta aggatccgtg tccaaaggag aggagttaat caaggaaaac atgagaatga
     3001 aagttgtcat ggagggctcc gttaatggtc accaattcaa gtgtacaggg gaaggtgaag
     3061 gtaatcctta catgggtaca caaactatga gaattaaagt aattgaaggc ggaccactac
     3121 catttgcatt tgacattctg gcaacgtcat tcatgtacgg atcacgaact ttcatcaagt
     3181 accctaaagg tataccagac tttttcaagc aatcttttcc agagggtttt acatgggaaa
     3241 gggttacaag atacgaagat gggggtgtcg tcacagttat gcaagatact tcattagaag
     3301 atggctgcct tgtctatcat gtgcaagtaa gaggggtgaa ttttccttct aacggacctg
     3361 tgatgcagaa aaagaccaaa ggttgggaac caaatactga aatgatgtac ccagctgatg
     3421 gaggtttgag aggctacaca cacatggcgc ttaaagttga tggtggaggt catttgtctt
     3481 gtagttttgt taccacttat cgttctaaaa agactgttgg caatatcaaa atgccaggaa
     3541 tacatgctgt agaccacaga ctagaaagac tcgaagagag cgataacgaa atgttcgttg
     3601 tacagagaga gcatgccgta gccaaatttg ctggcttagg cggtggtatg gatgaattgt
     3661 ataagtaact cgagtggcct cggtaccaaa ttccagaaaa gacacccgaa agggtgtttt
     3721 ttcgttttgg tccgctg    
//
