LOCUS       pLNL1143                4337 bp ds-DNA     circular     19-FEB-2017
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  . .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(1046..1193)
                     /label=KanR Promoter
                     /ApEinfo_fwdcolor="#93ff35"
                     /ApEinfo_revcolor="#93ff35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(230..1045)
                     /label=KanR
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(203..229)
                     /label=KanR Terminator
                     /ApEinfo_fwdcolor="#93ff35"
                     /ApEinfo_revcolor="#93ff35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    join(144..149,151..154)
                     /label=BsmBI
                     /ApEinfo_fwdcolor="#00ff15"
                     /ApEinfo_revcolor="#e72e00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(join(2062..2065,2067..2072))
                     /label=BsmBI(1)
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#00ff15"
                     /ApEinfo_revcolor="#e72e00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3558..4265
                     /label=mRuby2
                     /ApEinfo_fwdcolor="#FF454E"
                     /ApEinfo_revcolor="#FF454E"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1194..2034
                     /label=p15A
                     /ApEinfo_fwdcolor="#FAFFB7"
                     /ApEinfo_revcolor="#FAFFB7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2292..3551
                     /label=RsVS
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4279..4333
                     /label=tL3S2P24
                     /ApEinfo_fwdcolor="#b0b0ff"
                     /ApEinfo_revcolor="#b0b0ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2268..2270
                     /label=BBa_B0029
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2227..2261
                     /label=J23110
                     /ApEinfo_fwdcolor="#48a4ff"
                     /ApEinfo_revcolor="#48a4ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2286..2287
                     /label=BBa_B0029(1)
                     /ApEinfo_label="BBa_B0029"
                     /ApEinfo_fwdcolor="#ff6666"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gaaatctgct cgtcagtggt gctcacactg acgaatcatg tacagatcat accgatgact
       61 gcctggcgac tcacaactaa gcaagacagc cggaaccagc gccggcgaac accactgcat
      121 atatggcata tcacaacagt ccacgtctca agcagttaca gagatgttac gaaccactag
      181 tgcactgcag tacagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact
      241 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
      301 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
      361 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
      421 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
      481 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
      541 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
      601 ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
      661 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
      721 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
      781 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
      841 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
      901 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
      961 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag
     1021 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     1081 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     1141 acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtagcgga
     1201 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
     1261 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
     1321 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
     1381 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
     1441 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     1501 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1561 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     1621 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     1681 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
     1741 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
     1801 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
     1861 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
     1921 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac
     1981 gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttgcggcc
     2041 gcccctgaat tcgcatctag actgatgaga cgtggtagag ccacaaacag ccggtacaag
     2101 caacgatctc caggaccatc tgaatcatgc gcggatgaca cgaactcacg acggcgatca
     2161 cagacattaa cccacagtac agacactgcg acaacgtggc aattcgtcgc aatacaacgg
     2221 atttacttta cggctagctc agtcctaggt acaatgctag cgctagcttc nnnnNNNNNN
     2281 NNNNNtctat ggcaccgcag atggaaaaag tgagcgagga gctgattctg cctagctccc
     2341 cgaccccgca gagcttaaaa tgctacaaaa ttagccattt ggatcagctg ttgctgacat
     2401 gccacatccc ttttatcctg ttctacccta atccgttaga tagcaatctg gatccggcgc
     2461 agacgagcca acacttaaaa caatcgctgt cgaaagtgct tacacatttt tacccactgg
     2521 cgggacgtat taacgtgaat tcttcggtgg attgcaatga ttctggcgtt ccgtttgtag
     2581 aagcgcgtgt tcaagcgcaa ctgtctcagg caatccagaa cgtggttgag ctggaaaaat
     2641 tggatcagta cctgccaagt gctgcttacc ccggtggaaa aattgaagtt aacgaagatg
     2701 tcccgctggc cgtgaaaatc agtttctttg aatgtggagg caccgcaatt ggtgtcaacc
     2761 tcagccacaa gattgctgac gtgctgtcgc ttgctacgtt cctgaacgcc tggaccgcaa
     2821 cctgccgtgg agaaacggag atcgtcctgc ctaactttga cctggccgcc cgtcactttc
     2881 cgccggtaga taataccccg tcaccggaac tcgtcccgga tgaaaatgtc gtgatgaagc
     2941 gctttgtctt cgacaaagag aaaatcggcg cgttgcgcgc tcaggcgagc tctgcatccg
     3001 aagaaaaaaa ttttagccgc gtccaacttg tcgtggccta tatctggaaa catgtaattg
     3061 atgtgacgcg tgctaaatat ggcgcaaaaa acaagtttgt ggtagtacag gcagtcaacc
     3121 tgcgttcgcg tatgaacccc ccgctgccgc actacgcgat gggtaatatt gcgaccctgc
     3181 tgttcgccgc tgtcgacgcc gaatgggata aagattttcc cgacctcatt ggtccgttgc
     3241 gcacttctct tgaaaaaacc gaagacgatc ataatcatga acttttaaaa ggtatgacct
     3301 gtctttacga actggaacca caggaactgc tgagttttac aagctggtgt cgtctggggt
     3361 tttatgattt agattttggc tgggggaaac cgctgagcgc ctgtaccacg acattcccga
     3421 aacgtaacgc ggccttactg atggacacgc gttctggaga tggcgtcgag gcgtggcttc
     3481 caatggcgga agatgaaatg gcgatgctgc cggtggagct gctgtctttg gtggattccg
     3541 acttttcaaa aggatccgtg tccaaaggag aggagttaat caaggaaaac atgagaatga
     3601 aagttgtcat ggagggctcc gttaatggtc accaattcaa gtgtacaggg gaaggtgaag
     3661 gtaatcctta catgggtaca caaactatga gaattaaagt aattgaaggc ggaccactac
     3721 catttgcatt tgacattctg gcaacgtcat tcatgtacgg atcacgaact ttcatcaagt
     3781 accctaaagg tataccagac tttttcaagc aatcttttcc agagggtttt acatgggaaa
     3841 gggttacaag atacgaagat gggggtgtcg tcacagttat gcaagatact tcattagaag
     3901 atggctgcct tgtctatcat gtgcaagtaa gaggggtgaa ttttccttct aacggacctg
     3961 tgatgcagaa aaagaccaaa ggttgggaac caaatactga aatgatgtac ccagctgatg
     4021 gaggtttgag aggctacaca cacatggcgc ttaaagttga tggtggaggt catttgtctt
     4081 gtagttttgt taccacttat cgttctaaaa agactgttgg caatatcaaa atgccaggaa
     4141 tacatgctgt agaccacaga ctagaaagac tcgaagagag cgataacgaa atgttcgttg
     4201 tacagagaga gcatgccgta gccaaatttg ctggcttagg cggtggtatg gatgaattgt
     4261 ataagtaact cgagtggcct cggtaccaaa ttccagaaaa gacacccgaa agggtgtttt
     4321 ttcgttttgg tccgctg    
//
