LOCUS       Exported               10866 bp ds-DNA     circular SYN 21-SEP-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10866)
  AUTHORS   Myat T. Lin, William D. Stone, Vishalsingh Chaudhari, Maureen Hanson
  TITLE     Small subunits can determine enzyme kinetics of tobacco Rubisco 
            expressed in Escherichia coli
  JOURNAL   Nature Plants 2020, PMID: 32929197, DOI: 10.1038/s41477-020-00761-5
REFERENCE   2  (bases 1 to 10866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 21, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10866
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        104..208
                     /note="AmpR promoter"
     CDS             209..1069
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1240..1828
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
     CDS             complement(2258..2449)
                     /codon_start=1
                     /label=rop
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3181..4059)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4086..4370
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     RBS             4397..4419
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4431..5864
                     /codon_start=1
                     /product="Rubisco large subunit from N. tabacum"
                     /label=Nt-rbcL
                     /translation="MSPQTETKASVGFKAGVKEYKLTYYTPEYQTKDTDILAAFRVTPQ
                     PGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYRIERVVGEKDQYIAYVAYP
                     LDLFEEGSVTNMFTSIVGNVFGFKALRALRLEDLRIPPAYVKTFQGPPHGIQVERDKLN
                     KYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFCA
                     EALYKAQAETGEIKGHYLNATAGTCEEMIKRAVFARELGVPIVMHDYLTGGFTANTSLA
                     HYCRDNGLLLHIHRAMHAVIDRQKNHGIHFRVLAKALRMSGGDHIHSGTVVGKLEGERD
                     ITLGFVDLLRDDFVEQDRSRGIYFTQDWVSLPGVLPVASGGIHVWHMPALTEIFGDDSV
                     LQFGGGTLGHPWGNAPGAVANRVALEACVKARNEGRDLAQEGNEIIREACKWSPELAAA
                     CEVWKEIVFNFAAVDVLDK"
     terminator      6177..6263
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6355..6382
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        6435..6453
                     /label=T7P
                     /note="T7 promoter"
     protein_bind    6454..6478
                     /label=lacO
                     /bound_moiety="LacI"
                     /note="lac operator"
     RBS             6493..6515
     CDS             6523..8148
                     /codon_start=1
                     /label=At-cpn60-alpha
                     /note="CPN60A"
                     /translation="MNVKEIAFDQHSRAALQAGIDKLADCVGLTLGPRGRNVVLDEFGS
                     PKVVNDGVTIARAIELPNAMENAGAALIREVASKTNDSAGDGTTTASILAREIIKHGLL
                     SVTSGANPVSLKRGIDKTVQGLIEELQKKARPVKGRDDIRAVASISAGNDDLIGSMIAD
                     AIDKVGPDGVLSIESSSSFETTVEVEEGMEIDRGYISPQFVTNPEKLLAEFENARVLIT
                     DQKITAIKDIIPILEKTTQLRAPLLIIAEDVTGEALATLVVNKLRGVLNVVAVKAPGFG
                     ERRKAMLQDIAILTGAEYLAMDMSLLVENATIDQLGIARKVTISKDSTTLIADAASKDE
                     LQARIAQLKKELFETDSVYDSEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAK
                     NATFAAIEEGIVPGGGAALVHLSTVIPAIKETFEDADERLGADIVQKALLSPAALIAQN
                     AGVEGEVVVEKIMFSDWENGYNAMTDTYENLFEAGVIDPAKVTRCALQNAASVAGMVLT
                     TQAIVVDKPKPKAPAAAAPEGLMV"
     RBS             8164..8186
     CDS             8194..9837
                     /codon_start=1
                     /label=At-cpn60-beta
                     /note="CPN60B"
                     /translation="MAAKELHFNKDGTTIRRLQAGVNKLADLVGVTLGPKGRNVVLESK
                     YGSPRIVNDGVTVAREVELEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGFIAE
                     GVKVVAAGANPVLITRGIEKTAKALVTELKKMSKEVEDSELADVAAVSAGNNDEIGNMI
                     AEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDRGYISPYFVTDSEKMSVEFDNCKLL
                     LVDKKITNARDLVGVLEDAIRGGYPILIIAEDIEQEALATLVVNKLRGTLKIAALRAPG
                     FGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVLGNASKVVLTKETSTIVGDGSTQ
                     DAVKKRVTQIKNLIEQAEQDYEKEKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVED
                     ALNATKAAVEEGIVVGGGCTLLRLASKVDAIKATLDNDEEKVGADIVKRALSYPLKLIA
                     KNAGVNGSVVSEKVLSNDNVKFGYNAATGKYEDLMAAGIIDPTKVVRCCLEHAASVAKT
                     FLMSDCVVVEIKEPEPVPVGNPMDNSGYGY"
     RBS             9879..9901
     CDS             9909..10520
                     /codon_start=1
                     /label=At-cpn20
                     /note="CPN20"
                     /translation="MASVVAPKYTSIKPLGDRVLVKIKEAEEKTLGGILLPSTAQSKPQ
                     GGEVVAVGEGRTIGKNKIDITVPTGAQIIYSKYAGTEVEFNDVKHLILKEDDIVGILET
                     EDIKDLKPLNDRVFIKVAEAEEKTAGGLLLTETTKEKPSIGTVIAVGPGSLDEEGKITP
                     LPVSTGSTVLYSKYAGNDFKGKDGSNYIALRASDVMAILS"
     terminator      10607..10654
                     /label=T7 term
                     /note="T7 terminator"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cgaagcggca tgcataatgt gcctgtcaaa tggacgaagc
     3121 agggattctg caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa
     3181 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
     3241 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
     3301 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
     3361 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
     3421 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
     3481 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
     3541 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
     3601 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
     3661 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
     3721 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
     3781 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
     3841 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
     3901 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
     3961 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
     4021 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
     4081 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
     4141 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     4201 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     4261 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     4321 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     4381 ttgggctaga aataattttg tttaacttta agaaggagat atacataccc atgtcaccac
     4441 aaacagagac taaagcaagt gttggattca aagctggtgt taaagagtac aaattgactt
     4501 attatactcc tgagtaccaa accaaggata ctgatatatt ggcagcattc cgagtaactc
     4561 ctcaacctgg agttccacct gaagaagcag gggccgcggt agctgccgaa tcttctactg
     4621 gtacatggac aactgtatgg accgatggac ttaccagcct tgatcgttac aaagggcgat
     4681 gctaccgcat cgagcgtgtt gttggagaaa aagatcaata tattgcttat gtagcttacc
     4741 ctttagacct ttttgaagaa ggttctgtta ccaacatgtt tacttccatt gtaggtaacg
     4801 tatttgggtt caaagccctg cgcgctctac gtctggaaga tctgcgaatc cctcctgctt
     4861 atgttaaaac tttccaaggt ccgcctcatg ggatccaagt tgaaagagat aaattgaaca
     4921 agtatggtcg tcccctgttg ggatgtacta ttaaacctaa attggggtta tctgctaaaa
     4981 actacggtag agctgtttat gaatgtcttc gcggtggact tgattttacc aaagatgatg
     5041 agaacgtgaa ctcacaacca tttatgcgtt ggagagatcg tttcttattt tgtgccgaag
     5101 cactttataa agcacaggct gaaacaggtg aaatcaaagg gcattacttg aatgctactg
     5161 caggtacatg cgaagaaatg atcaaaagag ctgtatttgc tagagaattg ggcgttccga
     5221 tcgtaatgca tgactactta acggggggat tcaccgcaaa tactagcttg gctcattatt
     5281 gccgagataa tggtctactt cttcacatcc accgtgcaat gcatgcggtt attgatagac
     5341 agaagaatca tggtatccac ttccgggtat tagcaaaagc gttacgtatg tctggtggag
     5401 atcatattca ctctggtacc gtagtaggta aacttgaagg tgaaagagac ataactttgg
     5461 gctttgttga tttactgcgt gatgattttg ttgaacaaga tcgaagtcgc ggtatttatt
     5521 tcactcaaga ttgggtctct ttaccaggtg ttctacccgt ggcttcagga ggtattcacg
     5581 tttggcatat gcctgctctg accgagatct ttggggatga ttccgtacta cagttcggtg
     5641 gaggaacttt aggacatcct tggggtaatg cgccaggtgc cgtagctaat cgagtagctc
     5701 tagaagcatg tgtaaaagct cgtaatgaag gacgtgatct tgctcaggaa ggtaatgaaa
     5761 ttattcgcga ggcttgcaaa tggagcccgg aactagctgc tgcttgtgaa gtatggaaag
     5821 agatcgtatt taattttgca gcagtggacg ttttggataa gtaaactagt actaagcttg
     5881 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc
     5941 accatcacca ttgagtttaa acggtctcca gcttggctgt tttggcggat gagagaagat
     6001 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc
     6061 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
     6121 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
     6181 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     6241 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
     6301 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg
     6361 ccatcctgac ggatggcctt ttgccggcca cgatgcgtcc ggcgtagagg atcgagatct
     6421 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc
     6481 tctagaaata attttgttta actttaagaa ggagatatac atatgaatgt aaaggaaata
     6541 gcttttgacc agcactctag agctgctcta caagctggta ttgataagct tgctgattgt
     6601 gttggtctta cgcttggccc cagagggagg aatgttgtgt tggatgaatt tggaagtcct
     6661 aaggttgtga atgatggagt caccattgct agagctattg agttacctaa tgccatggaa
     6721 aatgctggtg cagcgcttat tcgtgaggtt gcgagtaaga ctaatgactc tgctggtgat
     6781 gggacaacca ctgcgtctat ccttgctcgg gagataatca aacatggatt gttgagcgtc
     6841 acttctggtg cgaatcccgt ttcactcaag aggggaattg ataagactgt tcaaggtctg
     6901 atcgaagagc ttcagaagaa agctaggccc gtgaaaggtc gtgatgacat cagagctgtg
     6961 gcttctatct ctgctggaaa tgatgacctt atagggtcaa tgattgctga tgccatcgac
     7021 aaagttggac ctgatggtgt tttgtccatt gaatcttcat cttcttttga gaccacggtc
     7081 gaagttgaag aagggatgga gattgacaga ggttacatct cgcctcagtt tgttacaaac
     7141 cctgagaaac tactagctga gtttgagaat gctagggtat tgatcactga tcagaagatc
     7201 actgcgatca aagacatcat ccctattttg gagaagacca ctcagcttcg tgctccgttg
     7261 ttgattattg cagaggatgt tactggtgaa gccttagcta cccttgtcgt gaacaagctt
     7321 cgtggtgtcc tcaatgttgt tgccgttaaa gcgccaggat ttggagaaag aagaaaagcc
     7381 atgcttcaag atattgcaat cttgacagga gctgagtacc tagccatgga catgagctta
     7441 ctggttgaaa acgcaaccat agatcaattg ggtattgccc gaaaagtcac gattagcaaa
     7501 gattcgacta ccctcattgc agatgcagct tccaaggacg aattacaagc tcggattgct
     7561 cagctgaaga aagaactatt tgagactgat tctgtgtatg actcagagaa gctcgctgag
     7621 agaattgcga agctatctgg aggtgttgct gtcattaaag tcggagcagc aactgaaact
     7681 gagcttgagg accgtaagct tcgtatcgag gatgcaaaga acgcaacatt tgctgctatc
     7741 gaggaaggca tagttccagg tggtggtgct gctttggtgc atctctccac tgttattccc
     7801 gccattaagg agacttttga ggatgctgat gaacgtttgg gagctgacat agtacaaaag
     7861 gcattgctgt caccagctgc tcttattgct cagaacgctg gagttgaagg agaagttgtt
     7921 gtggaaaaga ttatgttcag cgattgggag aatgggtaca acgcaatgac tgatacttac
     7981 gagaatctgt tcgaagctgg agtgattgat ccggctaaag tgacaagatg tgcgctacag
     8041 aacgctgctt ccgtagcagg aatggtactg accactcagg ccattgttgt tgacaaaccg
     8101 aaacccaagg ctcctgctgc tgctgctcct gagggtctca tggtgtaagg agctagaaat
     8161 aattttgttt aactttaaga aggagatata catatggcag caaaggaatt acatttcaac
     8221 aaagatggga ctaccattag gagacttcaa gctggtgtca acaagctcgc agatcttgtt
     8281 ggcgttacac ttggacccaa aggacgaaat gttgttcttg agagcaagta tggatcacca
     8341 cgaattgtta atgatggtgt gactgttgca agagaggttg agttggaaga cccggttgag
     8401 aacattggcg ctaagcttgt gaggcaagcg gctgccaaaa ccaatgactt ggctggtgac
     8461 ggtacaacaa catctgttgt tcttgcacaa ggttttatcg ctgagggtgt caaggtggta
     8521 gctgctggtg caaaccctgt tttgatcacc agaggtattg agaagacagc aaaggctttg
     8581 gttaccgagc tcaagaaaat gtctaaggag gttgaagaca gtgaacttgc agatgttgca
     8641 gctgttagtg cgggtaacaa cgacgaaatt ggaaatatga ttgctgaagc aatgagcaaa
     8701 gtgggcagga aaggtgtggt tacccttgag gagggtaaaa gtgccgagaa caacctctac
     8761 gtcgtagaag gaatgcaatt tgaccgtggt tatatctctc cttactttgt gacagacagc
     8821 gagaaaatgt ccgtcgagtt cgacaattgc aagttacttc tcgttgacaa gaaaattacc
     8881 aatgcaaggg atcttgttgg tgttttggag gatgcaatta gaggcggata cccaatatta
     8941 attattgctg aagacattga acaagaagct ttagcgactc ttgttgtaaa caagcttaga
     9001 ggtacactga agatagcagc tctcagagct ccagggtttg gagagcgcaa gagccaatac
     9061 cttgatgata ttgccattct aactggagca actgtgatcc gtgaggaagt tggtctttca
     9121 cttgacaaag ctggaaaaga ggttcttggt aatgcttcaa aggttgtcct cacaaaggag
     9181 acctcgacca tagtgggtga tggaagcaca caggatgcag tgaaaaagcg tgttacacag
     9241 attaaaaatc ttattgagca agcggagcaa gattacgaga aggagaaact caatgagaga
     9301 attgcaaagc tctctggtgg agttgctgtg attcaggttg gagcacaaac tgagacagaa
     9361 ctcaaagaga agaagttgag agttgaagat gctcttaatg caacaaaggc cgctgtcgag
     9421 gaaggtattg ttgttggtgg tggttgcact ctgcttcgtc ttgcttccaa ggttgatgcc
     9481 attaaagcca cgcttgataa tgatgaagaa aaggttggag cggatatagt taaaagagca
     9541 ctaagttacc ccctaaaact aatcgccaag aatgctggtg ttaatggaag tgtagttagc
     9601 gagaaggtgc tttccaacga taacgtgaaa ttcggttaca atgctgcaac cggcaagtac
     9661 gaggatttga tggctgcagg aatcatcgat ccaacaaagg ttgtgagatg ttgcttggaa
     9721 cacgcagctt cggttgcaaa gactttcttg atgtcggact gcgttgttgt tgagatcaag
     9781 gaacctgaac cagttcccgt cggcaaccca atggacaact caggttacgg atactaaggt
     9841 cgacgtcgac cggataacaa ttgccctcta gaaataattt tgtttaactt taagaaggag
     9901 atatacatat ggcctctgtt gttgccccta agtatacttc aattaagcca ttgggagatc
     9961 gagttttggt gaagatcaag gaggcagagg agaagacttt aggtggtatc ttacttccat
    10021 ccactgctca atcaaaacct caaggaggtg aagtcgttgc tgtgggtgaa ggaagaacta
    10081 ttgggaagaa caaaattgat atcactgtcc ctactggagc acaaattatc tactccaaat
    10141 acgcaggaac tgaggtggag ttcaatgatg tgaagcatct tatcctcaag gaagatgata
    10201 ttgttggcat tcttgagaca gaggacatca aagatctcaa acctttgaat gaccgagtct
    10261 ttattaaggt tgctgaggcg gaggagaaaa cagctggagg gttgttgtta accgagacta
    10321 ccaaagagaa gccttctatt ggcaccgtga tagcagttgg accgggttcc ctagacgagg
    10381 aaggtaaaat tacgcctcta ccagtatcaa ccggaagcac agtactttac tccaagtatg
    10441 ctggtaacga cttcaagggc aaagatggtt ccaactacat tgccctcaga gcttcagatg
    10501 tgatggctat actttcttag ttatgttata tctttgtaat ctgcaacaag ctgggtaccg
    10561 gtaccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
    10621 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata
    10681 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
    10741 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
    10801 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca
    10861 tgagaa
//
