LOCUS       Exported               10701 bp ds-DNA     circular SYN 21-SEP-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Myat T. Lin, William D. Stone, Vishalsingh Chaudhari, Maureen Hanson
  TITLE     Small subunits can determine enzyme kinetics of tobacco Rubisco 
            expressed in Escherichia coli
  JOURNAL   Nature Plants 2020, PMID: 32929197, DOI: 10.1038/s41477-020-00761-5
REFERENCE   2  (bases 1 to 10701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 21, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        104..208
                     /note="AmpR promoter"
     CDS             209..1069
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1240..1828
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
     CDS             complement(2258..2449)
                     /codon_start=1
                     /label=rop
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3181..4059)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4086..4370
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     RBS             4397..4419
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4431..5864
                     /codon_start=1
                     /product="Rubisco large subunit from N. tabacum"
                     /label=Nt-rbcL
                     /translation="MSPQTETKASVGFKAGVKEYKLTYYTPEYQTKDTDILAAFRVTPQ
                     PGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYRIERVVGEKDQYIAYVAYP
                     LDLFEEGSVTNMFTSIVGNVFGFKALRALRLEDLRIPPAYVKTFQGPPHGIQVERDKLN
                     KYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFCA
                     EALYKAQAETGEIKGHYLNATAGTCEEMIKRAVFARELGVPIVMHDYLTGGFTANTSLA
                     HYCRDNGLLLHIHRAMHAVIDRQKNHGIHFRVLAKALRMSGGDHIHSGTVVGKLEGERD
                     ITLGFVDLLRDDFVEQDRSRGIYFTQDWVSLPGVLPVASGGIHVWHMPALTEIFGDDSV
                     LQFGGGTLGHPWGNAPGAVANRVALEACVKARNEGRDLAQEGNEIIREACKWSPELAAA
                     CEVWKEIVFNFAAVDVLDK"
     terminator      6177..6263
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6355..6382
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        6435..6453
                     /label=T7P
                     /note="T7 promoter"
     protein_bind    6454..6478
                     /label=lacO
                     /bound_moiety="LacI"
                     /note="lac operator"
     CDS             6523..8145
                     /codon_start=1
                     /product="XM_016653834"
                     /label=Nt_cpn60alpha
                     /translation="MAKEIAFDQKSRSALQAGIDKLADAVGLTLGPRGRNVVLDEYGTP
                     KVVNDGVTIARAIELPDAMENAGAALIREVASKTNDSAGDGTTTASVLAREIIKLGLLS
                     VTSGANPVSLKKGIDKTVLGLIEELEKKARPVKGRDDIKAIASISAGNDESIGTMIADA
                     IDKVGPDGVLSIESSSSFETTVHVEEGMEIDRGYISPQFVTNPEKLIVEFENARVLVTD
                     QKISAIKDIIPLLEKTTQLRSPLLIIAEDVTGEALATLVVNKLRGILNVAAIKAPGFGE
                     RRKALLQDIAIVTGAEYQATDLGLLIEGTPVEALGIARKVTITKDSTTIIADAASKDEI
                     QARIAQLKKELSETDSVYDSEKLAERIAKLSGGVAVIKVGAATEAELEDRKLRIEDAKN
                     ATFAAIEEGIVPGGGAAFVHLSTYVPAIKDKLEDADEKLGADIIQRALVAPASLIAQNA
                     GIEGEVVVEKVREAEWEMGYNAMTDKYENLVDAGVIDPAKVTRCALQNSASVAGMVLTT
                     QAIVVEKAKPKAAAPAAPQGLSV"
     promoter        8173..8191
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             8223..8245
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             8253..8546
                     /codon_start=1
                     /label=groES
                     /note="groES gene from E. coli"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVGN
                     GRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     CDS             8298..8351
                     /codon_start=1
                     /product="GroES chaperone mobile loop that interacts with
                     GroEL"
                     /label=S loop
                     /translation="EVETKSAGGIVLTGSAAA"
     promoter        8629..8647
                     /label=T7P
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8648..8672
                     /label=lacO
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8721..10364
                     /codon_start=1
                     /product="99% identical to XM_016634979"
                     /label=Nt-cpn60-beta-2
                     /translation="MAAKELYFNKDGSAIKRLQVGVNKLADLVGVTLGPKGRNVVLESK
                     YGAPKIVNDGVTVAREVELEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGLVAE
                     GVKVVAAGANPVLITRGIEKTTKALVAELKNMSKEVEDSELADVAAVSAGNNSEVGSMI
                     AEAMSKVGRKGVVTLEEGKSAENSLRVVEGMQFDRGYISPYFVTDSEKMSVEYENCKLL
                     LVDKKITNARDLVGVLEDAIRNGYPILIIAEDIEQEALATLVVNKLRGALKVAALKAPG
                     FGERKSQYLDDIATLTGGTVIREELGLTLDKADKEVLGHASKVVLTKDATTIVGDGSTQ
                     EAVNKRVVQIKNLIEAADQDYEKEKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVED
                     ALNATKAAVEEGIVVGGGCTLLRLASKVDAIKETLENDEEKVGADIVKRALSYPLKLIA
                     KNAGVNGSVVSEKVLSSDNPKFGYNAATGNYEDLMAAGIIDPTKVVRCCLEHASSVAKT
                     FLMSDCVVVEIKEPEPAVAGNPMDNSGYGY"
     terminator      10438..10485
                     /label=T7 term
                     /note="T7 terminator"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cgaagcggca tgcataatgt gcctgtcaaa tggacgaagc
     3121 agggattctg caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa
     3181 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
     3241 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
     3301 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
     3361 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
     3421 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
     3481 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
     3541 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
     3601 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
     3661 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
     3721 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
     3781 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
     3841 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
     3901 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
     3961 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
     4021 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
     4081 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
     4141 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     4201 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     4261 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     4321 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     4381 ttgggctaga aataattttg tttaacttta agaaggagat atacataccc atgtcaccac
     4441 aaacagagac taaagcaagt gttggattca aagctggtgt taaagagtac aaattgactt
     4501 attatactcc tgagtaccaa accaaggata ctgatatatt ggcagcattc cgagtaactc
     4561 ctcaacctgg agttccacct gaagaagcag gggccgcggt agctgccgaa tcttctactg
     4621 gtacatggac aactgtatgg accgatggac ttaccagcct tgatcgttac aaagggcgat
     4681 gctaccgcat cgagcgtgtt gttggagaaa aagatcaata tattgcttat gtagcttacc
     4741 ctttagacct ttttgaagaa ggttctgtta ccaacatgtt tacttccatt gtaggtaacg
     4801 tatttgggtt caaagccctg cgcgctctac gtctggaaga tctgcgaatc cctcctgctt
     4861 atgttaaaac tttccaaggt ccgcctcatg ggatccaagt tgaaagagat aaattgaaca
     4921 agtatggtcg tcccctgttg ggatgtacta ttaaacctaa attggggtta tctgctaaaa
     4981 actacggtag agctgtttat gaatgtcttc gcggtggact tgattttacc aaagatgatg
     5041 agaacgtgaa ctcacaacca tttatgcgtt ggagagatcg tttcttattt tgtgccgaag
     5101 cactttataa agcacaggct gaaacaggtg aaatcaaagg gcattacttg aatgctactg
     5161 caggtacatg cgaagaaatg atcaaaagag ctgtatttgc tagagaattg ggcgttccga
     5221 tcgtaatgca tgactactta acggggggat tcaccgcaaa tactagcttg gctcattatt
     5281 gccgagataa tggtctactt cttcacatcc accgtgcaat gcatgcggtt attgatagac
     5341 agaagaatca tggtatccac ttccgggtat tagcaaaagc gttacgtatg tctggtggag
     5401 atcatattca ctctggtacc gtagtaggta aacttgaagg tgaaagagac ataactttgg
     5461 gctttgttga tttactgcgt gatgattttg ttgaacaaga tcgaagtcgc ggtatttatt
     5521 tcactcaaga ttgggtctct ttaccaggtg ttctacccgt ggcttcagga ggtattcacg
     5581 tttggcatat gcctgctctg accgagatct ttggggatga ttccgtacta cagttcggtg
     5641 gaggaacttt aggacatcct tggggtaatg cgccaggtgc cgtagctaat cgagtagctc
     5701 tagaagcatg tgtaaaagct cgtaatgaag gacgtgatct tgctcaggaa ggtaatgaaa
     5761 ttattcgcga ggcttgcaaa tggagcccgg aactagctgc tgcttgtgaa gtatggaaag
     5821 agatcgtatt taattttgca gcagtggacg ttttggataa gtaaactagt actaagcttg
     5881 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc
     5941 accatcacca ttgagtttaa acggtctcca gcttggctgt tttggcggat gagagaagat
     6001 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc
     6061 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
     6121 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
     6181 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     6241 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
     6301 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg
     6361 ccatcctgac ggatggcctt ttgccggcca cgatgcgtcc ggcgtagagg atcgagatct
     6421 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc
     6481 tctagcaata attttgttta actttaagaa ggagatatac atatggctaa agaaattgcc
     6541 tttgaccaga aatctaggag tgcccttcag gctggtatcg ataagctcgc tgatgctgtc
     6601 ggtctcactc ttggtcccag ggggaggaat gtagtgttgg atgaatatgg caccccaaag
     6661 gtggttaatg acggagttac aattgctcgt gctatagagc tacctgatgc tatggaaaat
     6721 gctggcgctg cccttatcag ggaggttgca agcaaaacta acgattcagc cggtgatgga
     6781 accacgactg catctgttct tgctcgcgaa atcattaaac ttggtctatt gagtgttaca
     6841 tctggtgcaa atccagtgtc tttgaagaag ggcattgaca agactgtact gggtttgatt
     6901 gaagagctag aaaagaaggc tagacctgtt aaaggtcgtg atgacatcaa agctattgct
     6961 tcgatctctg ctggaaatga tgaaagcatt ggcaccatga ttgcggatgc aattgacaaa
     7021 gtcggtccag atggtgtgtt gtccatcgag tcatcctcct cctttgagac aactgttcat
     7081 gttgaagaag gaatggagat tgatagagga tatatttccc cacaatttgt caccaaccct
     7141 gagaaattaa ttgttgagtt tgagaatgct agagtcttag ttacggatca gaagatctcg
     7201 gctatcaagg atattatccc cctattggaa aagacaactc agttacgctc tcctctgctc
     7261 attattgctg aggatgtcac cggggaagct ctggctactc ttgtcgtgaa caagctgcgg
     7321 ggtatactga atgttgccgc catcaaagct cctggatttg gtgaaaggag gaaggctctt
     7381 ctgcaagata ttgccattgt gacaggggct gagtaccaag caactgacct gggcctgctc
     7441 attgagggta ccccagttga agcacttgga attgccagaa aggtgaccat taccaaggat
     7501 tcaacaacca tcattgctga tgcagcatcg aaggacgaga tacaggctag gattgctcag
     7561 ctcaaaaagg agttgtccga gacagactcg gtttatgact ccgagaaact tgctgagaga
     7621 atcgccaagt tgtctggggg tgttgccgtc atcaaggtcg gagctgctac agaggctgag
     7681 cttgaggacc gcaagcttcg catcgaggat gcaaagaatg caacttttgc tgcaatcgaa
     7741 gagggaatag tacctggtgg tggtgctgcc tttgttcatc tatcaactta tgtccctgcc
     7801 attaaggaca agctcgaaga tgcagatgaa aagttgggag ctgacatcat tcaaagggca
     7861 ttagtagcac cagcatcttt gatagcccaa aatgctggaa ttgaagggga agtagtagtg
     7921 gagaaggtaa gggaagctga atgggagatg ggttataacg cgatgacaga caaatacgag
     7981 aatcttgttg atgctggagt gatcgatcca gccaaggtga caagatgtgc attgcagaat
     8041 tcagcatcag ttgcaggaat ggtacttaca acacaagcca ttgtggtcga gaaggcaaag
     8101 cctaaggcag ctgcacctgc tgctccacaa gggctctcag tgtgatactt ggcgcgcgga
     8161 taacaattgc cataatacga ctcactatag ggagaccaca acggtttccc tctagaaata
     8221 attttgttta actttaagaa ggagatatac atatgaatat tcgtccattg catgatcgcg
     8281 tgatcgtcaa gcgtaaagaa gttgaaacta aatctgctgg cggcatcgtt ctgaccggct
     8341 ctgcagcggc taaatccacc cgcggcgaag tgctggctgt cggcaatggc cgtatccttg
     8401 aaaatggcga agtgaagccg ctggatgtga aagttggcga catcgttatt ttcaacgatg
     8461 gctacggtgt gaaatctgag aagatcgaca atgaagaagt gttgatcatg tccgaaagcg
     8521 acattctggc aattgttgaa gcgtaatact tggcgcgccc agtgcggccg cataatgctt
     8581 aagtcgaaca gaaagtaatc gtattgtaca cggccgcata atcgaaatta atacgactca
     8641 ctatagggga attgtgagcg gataacaatt ccccatctta gtatattagt taagtataag
     8701 aaggagatat acatatacat atggcagcaa aggagttgta tttcaacaag gatggatcag
     8761 ctatcaagag gctgcaggtt ggtgtgaaca aactcgctga tctagttgga gttactcttg
     8821 gcccaaaagg caggaatgtt gttcttgaga gcaagtatgg tgctccaaag attgtcaatg
     8881 atggagttac tgttgccaga gaggttgagt tagaggatcc agtagaaaac attggtgcta
     8941 aactagtgag acaagctgct gccaaaacca atgatttggc tggtgatggg actacaacat
     9001 ctgtcgtact agcccagggc ctggttgctg aaggtgttaa ggtggttgca gctggtgcaa
     9061 accctgtcct gatcaccaga ggaattgaga agaccacaaa agccttagta gctgaattga
     9121 agaatatgtc aaaagaggta gaagacagtg aattagcaga tgttgctgca gtaagtgctg
     9181 ggaacaactc tgaagtaggg agtatgattg ctgaagccat gagcaaggtt ggaaggaaag
     9241 gtgttgtgac tcttgaagag ggaaaaagtg ctgaaaacag tctccgtgtg gttgaaggaa
     9301 tgcaatttga ccgtggttac atctcaccat acttcgttac tgatagtgag aaaatgtccg
     9361 ttgagtatga gaactgtaag ttattactgg ttgacaagaa gataacaaat gcaagagatc
     9421 ttgttggtgt cctggaagat gctatcagaa atggttaccc aattttaatt attgccgaag
     9481 atattgagca agaagcacta gccacccttg ttgtcaataa gcttagaggt gctttaaagg
     9541 ttgctgccct taaagctcct ggttttggtg aacgaaaaag ccagtatctt gatgacatag
     9601 caacccttac tggaggcact gtgattaggg aggaacttgg ccttacctta gacaaggctg
     9661 acaaggaagt tctaggccat gcttctaagg tagtgctgac taaggatgcc accacaattg
     9721 ttggtgatgg tagcactcag gaagcagtca ataagcgtgt tgtacagatt aaaaacctca
     9781 tagaggcggc agatcaagat tatgaaaaag aaaagctaaa tgaaagaatt gctaaattat
     9841 caggaggtgt ggctgttata caggttggag cacaaactga aactgaattg aaggaaaaga
     9901 agctgagggt ggaagatgct ctcaatgcaa caaaggcagc tgttgaggaa ggtattgttg
     9961 ttggtggagg gtgcacactc ctgcgacttg cttctaaagt tgatgccatc aaggagactc
    10021 ttgagaatga tgaggagaag gttggagctg atattgttaa aagagcgttg agctacccat
    10081 tgaagttgat tgctaaaaat gctggtgtta atggaagtgt tgtcagtgag aaggtgctct
    10141 ctagtgacaa tccaaaattt ggatacaatg ctgcaactgg caattatgaa gacttgatgg
    10201 ctgctggtat tattgaccca acaaaggtgg tgagatgttg tctagagcat gcgtcatcag
    10261 tggcgaagac attcttgatg tcagattgtg tggtggtaga gatcaaggag cctgaaccag
    10321 cagttgctgg caacccaatg gacaattcag gttatggata ctaattatgt tatatctttg
    10381 taatctgcaa cgtaccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
    10441 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
    10501 atatccggat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag
    10561 catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg
    10621 cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctag cttatcgatg
    10681 ataagctgtc aaacatgaga a
//
