LOCUS       Exported                9215 bp ds-DNA     circular SYN 21-SEP-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Myat T. Lin, William D. Stone, Vishalsingh Chaudhari, Maureen Hanson
  TITLE     Small subunits can determine enzyme kinetics of tobacco Rubisco 
            expressed in Escherichia coli
  JOURNAL   Nature Plants 2020, PMID: 32929197, DOI: 10.1038/s41477-020-00761-5
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 21, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        104..208
                     /note="AmpR promoter"
     CDS             209..1069
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1240..1828
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
     CDS             complement(2258..2449)
                     /codon_start=1
                     /label=rop
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        3128..3146
                     /label=T7P
                     /note="T7 promoter"
     protein_bind    3147..3171
                     /label=lacO
                     /bound_moiety="LacI"
                     /note="lac operator"
     CDS             3216..4838
                     /codon_start=1
                     /product="XM_016653834"
                     /label=Nt_cpn60alpha
                     /translation="MAKEIAFDQKSRSALQAGIDKLADAVGLTLGPRGRNVVLDEYGTP
                     KVVNDGVTIARAIELPDAMENAGAALIREVASKTNDSAGDGTTTASVLAREIIKLGLLS
                     VTSGANPVSLKKGIDKTVLGLIEELEKKARPVKGRDDIKAIASISAGNDESIGTMIADA
                     IDKVGPDGVLSIESSSSFETTVHVEEGMEIDRGYISPQFVTNPEKLIVEFENARVLVTD
                     QKISAIKDIIPLLEKTTQLRSPLLIIAEDVTGEALATLVVNKLRGILNVAAIKAPGFGE
                     RRKALLQDIAIVTGAEYQATDLGLLIEGTPVEALGIARKVTITKDSTTIIADAASKDEI
                     QARIAQLKKELSETDSVYDSEKLAERIAKLSGGVAVIKVGAATEAELEDRKLRIEDAKN
                     ATFAAIEEGIVPGGGAAFVHLSTYVPAIKDKLEDADEKLGADIIQRALVAPASLIAQNA
                     GIEGEVVVEKVREAEWEMGYNAMTDKYENLVDAGVIDPAKVTRCALQNSASVAGMVLTT
                     QAIVVEKAKPKAAAPAAPQGLSV"
     RBS             4878..4900
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4908..5522
                     /codon_start=1
                     /product="XM_016634378"
                     /label=Nt-cpn20
                     /translation="MAATVVAPKYTSLKPLGDRVLVKIKTVEEKTVGGILLPTTAQSKP
                     QGGEVVAVGEGRSVGKNQVDISVKTGTQVVYSKYAGTEVEFNGSNHLILKEDDIVGILE
                     TDEIKDLQPLNDRVLIKVAEAEEKTAGGLLLTEASKEKPSIGTVIAVGPGPLDEEGNRK
                     PLSVSTGNTVLYSKYAGNEFKGADGSEYIALRASDVMAVLS"
     promoter        5535..5553
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5554..5578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5593..5615
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5627..7060
                     /codon_start=1
                     /product="Rubisco large subunit from N. tabacum"
                     /label=Nt-rbcL
                     /translation="MSPQTETKASVGFKAGVKEYKLTYYTPEYQTKDTDILAAFRVTPQ
                     PGVPPEEAGAAVAAESSTGTWTTVWTDGLTSLDRYKGRCYRIERVVGEKDQYIAYVAYP
                     LDLFEEGSVTNMFTSIVGNVFGFKALRALRLEDLRIPPAYVKTFQGPPHGIQVERDKLN
                     KYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFCA
                     EALYKAQAETGEIKGHYLNATAGTCEEMIKRAVFARELGVPIVMHDYLTGGFTANTSLA
                     HYCRDNGLLLHIHRAMHAVIDRQKNHGIHFRVLAKALRMSGGDHIHSGTVVGKLEGERD
                     ITLGFVDLLRDDFVEQDRSRGIYFTQDWVSLPGVLPVASGGIHVWHMPALTEIFGDDSV
                     LQFGGGTLGHPWGNAPGAVANRVALEACVKARNEGRDLAQEGNEIIREACKWSPELAAA
                     CEVWKEIVFNFAAVDVLDK"
     promoter        7143..7161
                     /label=T7P
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7162..7186
                     /label=lacO
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             7235..8878
                     /codon_start=1
                     /product="99% identical to XM_016634979"
                     /label=Nt-cpn60-beta-2
                     /translation="MAAKELYFNKDGSAIKRLQVGVNKLADLVGVTLGPKGRNVVLESK
                     YGAPKIVNDGVTVAREVELEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGLVAE
                     GVKVVAAGANPVLITRGIEKTTKALVAELKNMSKEVEDSELADVAAVSAGNNSEVGSMI
                     AEAMSKVGRKGVVTLEEGKSAENSLRVVEGMQFDRGYISPYFVTDSEKMSVEYENCKLL
                     LVDKKITNARDLVGVLEDAIRNGYPILIIAEDIEQEALATLVVNKLRGALKVAALKAPG
                     FGERKSQYLDDIATLTGGTVIREELGLTLDKADKEVLGHASKVVLTKDATTIVGDGSTQ
                     EAVNKRVVQIKNLIEAADQDYEKEKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVED
                     ALNATKAAVEEGIVVGGGCTLLRLASKVDAIKETLENDEEKVGADIVKRALSYPLKLIA
                     KNAGVNGSVVSEKVLSSDNPKFGYNAATGNYEDLMAAGIIDPTKVVRCCLEHASSVAKT
                     FLMSDCVVVEIKEPEPAVAGNPMDNSGYGY"
     terminator      8952..8999
                     /label=T7 term
                     /note="T7 terminator"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
     3121 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagca
     3181 ataattttgt ttaactttaa gaaggagata tacatatggc taaagaaatt gcctttgacc
     3241 agaaatctag gagtgccctt caggctggta tcgataagct cgctgatgct gtcggtctca
     3301 ctcttggtcc cagggggagg aatgtagtgt tggatgaata tggcacccca aaggtggtta
     3361 atgacggagt tacaattgct cgtgctatag agctacctga tgctatggaa aatgctggcg
     3421 ctgcccttat cagggaggtt gcaagcaaaa ctaacgattc agccggtgat ggaaccacga
     3481 ctgcatctgt tcttgctcgc gaaatcatta aacttggtct attgagtgtt acatctggtg
     3541 caaatccagt gtctttgaag aagggcattg acaagactgt actgggtttg attgaagagc
     3601 tagaaaagaa ggctagacct gttaaaggtc gtgatgacat caaagctatt gcttcgatct
     3661 ctgctggaaa tgatgaaagc attggcacca tgattgcgga tgcaattgac aaagtcggtc
     3721 cagatggtgt gttgtccatc gagtcatcct cctcctttga gacaactgtt catgttgaag
     3781 aaggaatgga gattgataga ggatatattt ccccacaatt tgtcaccaac cctgagaaat
     3841 taattgttga gtttgagaat gctagagtct tagttacgga tcagaagatc tcggctatca
     3901 aggatattat ccccctattg gaaaagacaa ctcagttacg ctctcctctg ctcattattg
     3961 ctgaggatgt caccggggaa gctctggcta ctcttgtcgt gaacaagctg cggggtatac
     4021 tgaatgttgc cgccatcaaa gctcctggat ttggtgaaag gaggaaggct cttctgcaag
     4081 atattgccat tgtgacaggg gctgagtacc aagcaactga cctgggcctg ctcattgagg
     4141 gtaccccagt tgaagcactt ggaattgcca gaaaggtgac cattaccaag gattcaacaa
     4201 ccatcattgc tgatgcagca tcgaaggacg agatacaggc taggattgct cagctcaaaa
     4261 aggagttgtc cgagacagac tcggtttatg actccgagaa acttgctgag agaatcgcca
     4321 agttgtctgg gggtgttgcc gtcatcaagg tcggagctgc tacagaggct gagcttgagg
     4381 accgcaagct tcgcatcgag gatgcaaaga atgcaacttt tgctgcaatc gaagagggaa
     4441 tagtacctgg tggtggtgct gcctttgttc atctatcaac ttatgtccct gccattaagg
     4501 acaagctcga agatgcagat gaaaagttgg gagctgacat cattcaaagg gcattagtag
     4561 caccagcatc tttgatagcc caaaatgctg gaattgaagg ggaagtagta gtggagaagg
     4621 taagggaagc tgaatgggag atgggttata acgcgatgac agacaaatac gagaatcttg
     4681 ttgatgctgg agtgatcgat ccagccaagg tgacaagatg tgcattgcag aattcagcat
     4741 cagttgcagg aatggtactt acaacacaag ccattgtggt cgagaaggca aagcctaagg
     4801 cagctgcacc tgctgctcca caagggctct cagtgtgata cttggcgcgc ggataacaat
     4861 tgccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg gctgctactg
     4921 ttgtagcccc caaatacact tctcttaagc ctttgggtga tagagttttg gtgaagatta
     4981 agactgtaga ggaaaagacc gtaggtggta tcctacttcc aacaacagca cagtcgaaac
     5041 ctcaaggagg tgaggttgtt gctgttgggg agggtcgttc agttgggaag aatcaggtgg
     5101 acattagtgt gaagactggc acccaagttg tttactcaaa atatgcggga acagaggtgg
     5161 agttcaatgg atcaaatcac ctcatcttga aggaggatga cattgttggt attctcgaga
     5221 cagatgaaat caaggatctg cagcccttga atgacagagt tctgataaag gtcgccgagg
     5281 ctgaggaaaa aactgctgga ggcttattat taactgaagc atccaaggag aagccttcca
     5341 ttggcacggt tatagctgta ggtcctggtc cacttgacga ggaagggaac cggaaaccac
     5401 tatcagtatc cacaggaaat acagttctat actccaaata tgctggcaat gaattcaaag
     5461 gtgctgatgg ttctgagtac attgcactga gggcatctga tgtaatggct gtgctttcat
     5521 agggcgcgcg aagttaatac gactcactat aggggaattg tgagcggata acaattcccc
     5581 tctagaaata attttgttta actttaagaa ggagatatac atacccatgt caccacaaac
     5641 agagactaaa gcaagtgttg gattcaaagc tggtgttaaa gagtacaaat tgacttatta
     5701 tactcctgag taccaaacca aggatactga tatattggca gcattccgag taactcctca
     5761 acctggagtt ccacctgaag aagcaggggc cgcggtagct gccgaatctt ctactggtac
     5821 atggacaact gtatggaccg atggacttac cagccttgat cgttacaaag ggcgatgcta
     5881 ccgcatcgag cgtgttgttg gagaaaaaga tcaatatatt gcttatgtag cttacccttt
     5941 agaccttttt gaagaaggtt ctgttaccaa catgtttact tccattgtag gtaacgtatt
     6001 tgggttcaaa gccctgcgcg ctctacgtct ggaagatctg cgaatccctc ctgcttatgt
     6061 taaaactttc caaggtccgc ctcatgggat ccaagttgaa agagataaat tgaacaagta
     6121 tggtcgtccc ctgttgggat gtactattaa acctaaattg gggttatctg ctaaaaacta
     6181 cggtagagct gtttatgaat gtcttcgcgg tggacttgat tttaccaaag atgatgagaa
     6241 cgtgaactca caaccattta tgcgttggag agatcgtttc ttattttgtg ccgaagcact
     6301 ttataaagca caggctgaaa caggtgaaat caaagggcat tacttgaatg ctactgcagg
     6361 tacatgcgaa gaaatgatca aaagagctgt atttgctaga gaattgggcg ttccgatcgt
     6421 aatgcatgac tacttaacgg ggggattcac cgcaaatact agcttggctc attattgccg
     6481 agataatggt ctacttcttc acatccaccg tgcaatgcat gcggttattg atagacagaa
     6541 gaatcatggt atccacttcc gggtattagc aaaagcgtta cgtatgtctg gtggagatca
     6601 tattcactct ggtaccgtag taggtaaact tgaaggtgaa agagacataa ctttgggctt
     6661 tgttgattta ctgcgtgatg attttgttga acaagatcga agtcgcggta tttatttcac
     6721 tcaagattgg gtctctttac caggtgttct acccgtggct tcaggaggta ttcacgtttg
     6781 gcatatgcct gctctgaccg agatctttgg ggatgattcc gtactacagt tcggtggagg
     6841 aactttagga catccttggg gtaatgcgcc aggtgccgta gctaatcgag tagctctaga
     6901 agcatgtgta aaagctcgta atgaaggacg tgatcttgct caggaaggta atgaaattat
     6961 tcgcgaggct tgcaaatgga gcccggaact agctgctgct tgtgaagtat ggaaagagat
     7021 cgtatttaat tttgcagcag tggacgtttt ggataagtaa tacttggcgc gcccagtgcg
     7081 gccgcataat gcttaagtcg aacagaaagt aatcgtattg tacacggccg cataatcgaa
     7141 attaatacga ctcactatag gggaattgtg agcggataac aattccccat cttagtatat
     7201 tagttaagta taagaaggag atatacatat acatatggca gcaaaggagt tgtatttcaa
     7261 caaggatgga tcagctatca agaggctgca ggttggtgtg aacaaactcg ctgatctagt
     7321 tggagttact cttggcccaa aaggcaggaa tgttgttctt gagagcaagt atggtgctcc
     7381 aaagattgtc aatgatggag ttactgttgc cagagaggtt gagttagagg atccagtaga
     7441 aaacattggt gctaaactag tgagacaagc tgctgccaaa accaatgatt tggctggtga
     7501 tgggactaca acatctgtcg tactagccca gggcctggtt gctgaaggtg ttaaggtggt
     7561 tgcagctggt gcaaaccctg tcctgatcac cagaggaatt gagaagacca caaaagcctt
     7621 agtagctgaa ttgaagaata tgtcaaaaga ggtagaagac agtgaattag cagatgttgc
     7681 tgcagtaagt gctgggaaca actctgaagt agggagtatg attgctgaag ccatgagcaa
     7741 ggttggaagg aaaggtgttg tgactcttga agagggaaaa agtgctgaaa acagtctccg
     7801 tgtggttgaa ggaatgcaat ttgaccgtgg ttacatctca ccatacttcg ttactgatag
     7861 tgagaaaatg tccgttgagt atgagaactg taagttatta ctggttgaca agaagataac
     7921 aaatgcaaga gatcttgttg gtgtcctgga agatgctatc agaaatggtt acccaatttt
     7981 aattattgcc gaagatattg agcaagaagc actagccacc cttgttgtca ataagcttag
     8041 aggtgcttta aaggttgctg cccttaaagc tcctggtttt ggtgaacgaa aaagccagta
     8101 tcttgatgac atagcaaccc ttactggagg cactgtgatt agggaggaac ttggccttac
     8161 cttagacaag gctgacaagg aagttctagg ccatgcttct aaggtagtgc tgactaagga
     8221 tgccaccaca attgttggtg atggtagcac tcaggaagca gtcaataagc gtgttgtaca
     8281 gattaaaaac ctcatagagg cggcagatca agattatgaa aaagaaaagc taaatgaaag
     8341 aattgctaaa ttatcaggag gtgtggctgt tatacaggtt ggagcacaaa ctgaaactga
     8401 attgaaggaa aagaagctga gggtggaaga tgctctcaat gcaacaaagg cagctgttga
     8461 ggaaggtatt gttgttggtg gagggtgcac actcctgcga cttgcttcta aagttgatgc
     8521 catcaaggag actcttgaga atgatgagga gaaggttgga gctgatattg ttaaaagagc
     8581 gttgagctac ccattgaagt tgattgctaa aaatgctggt gttaatggaa gtgttgtcag
     8641 tgagaaggtg ctctctagtg acaatccaaa atttggatac aatgctgcaa ctggcaatta
     8701 tgaagacttg atggctgctg gtattattga cccaacaaag gtggtgagat gttgtctaga
     8761 gcatgcgtca tcagtggcga agacattctt gatgtcagat tgtgtggtgg tagagatcaa
     8821 ggagcctgaa ccagcagttg ctggcaaccc aatggacaat tcaggttatg gatactaatt
     8881 atgttatatc tttgtaatct gcaacgtacc gaaaggaagc tgagttggct gctgccaccg
     8941 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     9001 tgaaaggagg aactatatcc ggatatcccg caagaggccc ggcagtaccg gcataaccaa
     9061 gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga
     9121 tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag
     9181 ctagcttatc gatgataagc tgtcaaacat gagaa
//
