LOCUS       POPLZZZ2_pSH100_MC        7662 bp ds-DNA   linear       28-APR-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    421..423
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=Start Codon
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     misc_feature    385..414
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=MET25 promoter?
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#ff8080
     misc_feature    2648..2887
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=CYC1 terminator
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#c0c0c0
     misc_feature    5616..6476
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=AmpR
                     /ApEinfo_fwdcolor=#ffff80
                     /ApEinfo_revcolor=#ffff80
     misc_feature    6631..7250
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=pBR322Ori
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=#808000
     misc_feature    join(436..692,694..786)
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=MS2 coat protein
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
     misc_feature    3685..4485
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=New Feature
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=#008080
     misc_feature    2596..2614
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=MS2 hairpin
                     /ApEinfo_fwdcolor=#fc293f
                     /ApEinfo_revcolor=#fc293f
     misc_feature    2043..2348
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=ccdB
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#ff8080
     misc_feature    2389..2511
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=R2
                     /ApEinfo_fwdcolor=#80ff00
                     /ApEinfo_revcolor=#80ff00
     misc_feature    2551..2553
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=Stop Codon
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     misc_feature    2570..2595
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=3'UTR
                     /ApEinfo_fwdcolor=#ffd1d1
                     /ApEinfo_revcolor=#ffd1d1
     misc_feature    808..932
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=R1
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
     misc_feature    808..819
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=Primer1
                     /ApEinfo_fwdcolor=#ffff80
                     /ApEinfo_revcolor=#ffff80
     misc_feature    2533..2550
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=6xHis
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=#ff80ff
     misc_feature    2615..2634
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=3'UTR(1)
                     /ApEinfo_label=3'UTR
                     /ApEinfo_fwdcolor=#ffd1d1
                     /ApEinfo_revcolor=#ffd1d1
     misc_feature    1042..1701
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=cmlR
                     /ApEinfo_fwdcolor=#808040
                     /ApEinfo_revcolor=#808040
     misc_feature    2502..2511
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /label=Primer2
                     /ApEinfo_fwdcolor=#ffff80
                     /ApEinfo_revcolor=#ffff80
ORIGIN
        1 ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCGGATGCA AGGGTTCGAA TCCCTTAGCT
       61 CTCATTATTT TTTGCTTTTT CTCTTGAGGT CACATGATCG CAAAATGGCA AATGGCACGT
      121 GAAGCTGTCG ATATTGGGGA ACTGTGGTGG TTGGCAAATG ACTAATTAAG TTAGTCAAGG
      181 CGCCATCCTC ATGAAAACTG TGTAACATAA TAACCGAAGT GTCGAAAAGG TGGCACCTTG
      241 TCCAATTGAA CACGCTCGAT GAAAAAAATA AGATATATAT AAGGTTAAGT AAAGCGTCTG
      301 TTAGAAAGGA AGTTTTTCCT TTTTCTTGCT CTCTTGTCTT TTCATCTACT ATTTCCTTCG
      361 TGTAATACAG GGTCGTCAGA TACATAGATA CAATTCTATT ACCCCCATCC ATACTCTAGA
      421 ATGCTAGCCG TTAAAATGGC TTCTAACTTT ACTCAGTTCG TTCTCGTCGA CAATGGCGGA
      481 ACTGGCGACG TGACTGTCGC CCCAAGCAAC TTCGCTAACG GGATCGCTGA ATGGATCAGC
      541 TCTAACTCGC GTTCACAGGC TTACAAAGTA ACCTGTAGCG TTCGTCAGAG CTCTGCGCAG
      601 AATCGCAAAT ACACCATCAA AGTCGAGGTG CCTAAAGGCG CCTGGCGTTC GTACTTAAAT
      661 ATGGAACTAA CCATTCCAAT TTTCGCCACG AATTCCGACT GCGAGCTTAT TGTTAAGGCA
      721 ATGCAAGGTC TCCTAAAAGA TGGAAACCCG ATTCCCTCAG CAATCGCAGC AAACTCCGGC
      781 ATCTACGCGG ATCCACCGGT CGCCACCACA AGTTTGTACA AAAAAGCTGA ACGAGAAACG
      841 TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA
      901 TACTGTAAAA CACAACATAT CCAGTCACTA TGGCGGCCGC ATTAGGGCAC CCCAGGCTTT
      961 ACACTTTATG CTTCCGGCTC GTATAATGTG TGGATTTTGA GTTAGGATCC GTCGGGATTT
     1021 TCAGGAGCTA AGGAAGCTAA AATGGAGAAA AAAATCACTG GATATACCAC CGTTGATATA
     1081 TCCCAATGGC ATCGTAAAGA ACATTTTGAG GCATTTCAGT CAGTTGCTCA ATGTACCTAT
     1141 AACCAGACCG TTCAGCTGGA TATTACGGCC TTTTTAAAGA CCGTAAAGAA AAATAAGCAC
     1201 AAGTTTTATC CGGCCTTTAT TCACATTCTT GCCCGCCTGA TGAATGCTCA TCCGGAATTC
     1261 CGTATGGCAA TGAAAGACGG TGAGCTGGTG ATATGGGATA GTGTTCACCC TTGTTACACC
     1321 GTTTTCCATG AGCAAACTGA AACGTTTTCA TCGCTCTGGA GTGAATACCA CGACGATTTC
     1381 CGGCAGTTTC TACACATATA TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT
     1441 TTCCCTAAAG GGTTTATTGA GAATATGTTT TTCGTCTCAG CCAATCCCTG GGTGAGTTTC
     1501 ACCAGTTTTG ATTTAAACGT GGCCAATATG GACAACTTCT TCGCCCCCGT TTTCACCATG
     1561 GGCAAATATT ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT
     1621 GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA ATGCTTAATG AATTACAACA GTACTGCGAT
     1681 GAGTGGCAGG GCGGGGCGTA ATCTAGAGGA TCCGGCTTAC TAAAAGCCAG ATAACAGTAT
     1741 GCGTATTTGC GCGCTGATTT TTGCGGTATA AGAATATATA CTGATATGTA TACCCGAAGT
     1801 ATGTCAAAAA GAGGTGTGCT ATGAAGCAGC GTATTACAGT GACAGTTGAC AGCGACAGCT
     1861 ATCAGTTGCT CAAGGCATAT ATGATGTCAA TATCTCCGGT CTGGTAAGCA CAACCATGCA
     1921 GAATGAAGCC CGTCGTCTGC GTGCCGAACG CTGGAAAGCG GAAAATCAGG AAGGGATGGC
     1981 TGAGGTCGCC CGGTTTATTG AAATGAACGG CTCTTTTGCT GACGAGAACA GGGACTGGTG
     2041 AAATGCAGTT TAAGGTTTAC ACCTATAAAA GAGAGAGCCG TTATCGTCTG TTTGTGGATG
     2101 TACAGAGTGA TATTATTGAC ACGCCCGGGC GACGGATGGT GATCCCCCTG GCCAGTGCAC
     2161 GTCTGCTGTC AGATAAAGTC CCCCGTGAAC TTTACCCGGT GGTGCATATC GGGGATGAAA
     2221 GCTGGCGCAT GATGACCACC GATATGGCCA GTGTGCCGGT CTCCGTTATC GGGGAAGAAG
     2281 TGGCTGATCT CAGCCACCGC GAAAATGACA TCAAAAACGC CATTAACCTG ATGTTCTGGG
     2341 GAATATAAAT GTCAGGCTCC CTTATACACA GCCAGTCTGC AGGTCGACCA TAGTGACTGG
     2401 ATATGTTGTG TTTTACAGTA TTATGTAGTC TGTTTTTTAT GCAAAATCTA ATTTAATATA
     2461 TTGATATTTA TATCATTTTA CGTTTCTCGT TCAGCTTTCT TGTACAAAGT GGTTTCAAGA
     2521 GGGCCCTTCG AACATCATCA CCATCACCAT TAAATCGATA CCGTCGACGA TCCTACGGTA
     2581 CTTATTGCCA AGAAAGCACG AGCATCAGCC GTGCCTCCAG GTCGAATCTT CAAACTCGAG
     2641 TCATGTAATT AGTTATGTCA CGCTTACATT CACGCCCTCC CCCCACATCC GCTCTAACCG
     2701 AAAAGGAAGG AGTTAGACAA CCTGAAGTCT AGGTCCCTAT TTATTTTTTT ATAGTTATGT
     2761 TAGTATTAAG AACGTTATTT ATATTTCAAA TTTTTCTTTT TTTTCTGTAC AGACGCGTGT
     2821 ACGCATGTAA CATTATACTG AAAACCTTGC TTGAGAAGGT TTTGGGACGC TCGAAGGCTT
     2881 TAATTTGCGG CCGGTACCCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC GCTCACTGGC
     2941 CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC
     3001 AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC
     3061 CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCGACGCG CCCTGTAGCG GCGCATTAAG
     3121 CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC
     3181 CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC
     3241 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA
     3301 AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG
     3361 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC
     3421 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA
     3481 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC
     3541 GTTTACAATT TCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC
     3601 ATAGGGTAAT AACTGATATA ATTAAATTGA AGCTCTAATT TGTGAGTTTA GTATACATGC
     3661 ATTTACTTAT AATACAGTTT TTTAGTTTTG CTGGCCGCAT CTTCTCAAAT ATGCTTCCCA
     3721 GCCTGCTTTT CTGTAACGTT CACCCTCTAC CTTAGCATCC CTTCCCTTTG CAAATAGTCC
     3781 TCTTCCAACA ATAATAATGT CAGATCCTGT AGAGACCACA TCATCCACGG TTCTATACTG
     3841 TTGACCCAAT GCGTCTCCCT TGTCATCTAA ACCCACACCG GGTGTCATAA TCAACCAATC
     3901 GTAACCTTCA TCTCTTCCAC CCATGTCTCT TTGAGCAATA AAGCCGATAA CAAAATCTTT
     3961 GTCGCTCTTC GCAATGTCAA CAGTACCCTT AGTATATTCT CCAGTAGATA GGGAGCCCTT
     4021 GCATGACAAT TCTGCTAACA TCAAAAGGCC TCTAGGTTCC TTTGTTACTT CTTCTGCCGC
     4081 CTGCTTCAAA CCGCTAACAA TACCTGGGCC CACCACACCG TGTGCATTCG TAATGTCTGC
     4141 CCATTCTGCT ATTCTGTATA CACCCGCAGA GTACTGCAAT TTGACTGTAT TACCAATGTC
     4201 AGCAAATTTT CTGTCTTCGA AGAGTAAAAA ATTGTACTTG GCGGATAATG CCTTTAGCGG
     4261 CTTAACTGTG CCCTCCATGG AAAAATCAGT CAAGATATCC ACATGTGTTT TTAGTAAACA
     4321 AATTTTGGGA CCTAATGCTT CAACTAACTC CAGTAATTCC TTGGTGGTAC GAACATCCAA
     4381 TGAAGCACAC AAGTTTGTTT GCTTTTCGTG CATGATATTA AATAGCTTGG CAGCAACAGG
     4441 ACTAGGATGA GTAGCAGCAC GTTCCTTATA TGTAGCTTTC GACATGATTT ATCTTCGTTT
     4501 CCTGCAGGTT TTTGTTCTGT GCAGTTGGGT TAAGAATACT GGGCAATTTC ATGTTTCTTC
     4561 AACACTACAT ATGCGTATAT ATACCAATCT AAGTCTGTGC TCCTTCCTTC GTTCTTCCTT
     4621 CTGTTCGGAG ATTACCGAAT CAAAAAAATT TCAAAGAAAC CGAAATCAAA AAAAAGAATA
     4681 AAAAAAAAAT GATGAATTGA ATTGAAAAGC TGTGGTATGG TGCACTCTCA GTACAATCTG
     4741 CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG
     4801 ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG
     4861 CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT
     4921 ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGGACG GATCGCTTGC
     4981 CTGTAACTTA CACGCGCCTC GTATCTTTTA ATGATGGAAT AATTTGGGAA TTTACTCTGT
     5041 GTTTATTTAT TTTTATGTTT TGTATTTGGA TTTTAGAAAG TAAATAAAGA AGGTAGAAGA
     5101 GTTACGGAAT GAAGAAAAAA AAATAAACAA AGGTTTAAAA AATTTCAACA AAAAGCGTAC
     5161 TTTACATATA TATTTATTAG ACAAGAAAAG CAGATTAAAT AGATATACAT TCGATTAACG
     5221 ATAAGTAAAA TGTAAAATCA CAGGATTTTC GTGTGTGGTC TTCTACACAG ACAAGATGAA
     5281 ACAATTCGGC ATTAATACCT GAGAGCAGGA AGAGCAAGAT AAAAGGTAGT ATTTGTTGGC
     5341 GATCCCCCTA GAGTCTTTTA CATCTTCGGA AAACAAAAAC TATTTTTTCT TTAATTTCTT
     5401 TTTTTACTTT CTATTTTTAA TTTATATATT TATATTAAAA AATTTAAATT ATAATTATTT
     5461 TTATAGCACG TGATGAAAAG GACCCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC
     5521 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT
     5581 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG
     5641 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG
     5701 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC
     5761 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA
     5821 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC
     5881 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA
     5941 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG
     6001 ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT
     6061 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG
     6121 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC
     6181 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA
     6241 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA
     6301 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC
     6361 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG
     6421 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT
     6481 CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA
     6541 GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT
     6601 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT
     6661 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT
     6721 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA
     6781 TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG
     6841 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA
     6901 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG
     6961 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA
     7021 GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA
     7081 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA
     7141 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT
     7201 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC
     7261 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT
     7321 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA
     7381 CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC
     7441 TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG
     7501 CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTACCTCAC TCATTAGGCA CCCCAGGCTT
     7561 TACACTTTAT GCTTCCGGCT CCTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA
     7621 CAGGAAACAG CTATGACCAT GATTACGCCA AGCGCGCAAT TA 
//
