LOCUS       POPL163_MCP_attB        6726 bp ds-DNA     circular     15-APR-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    421..423
                     /label=Start Codon
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    809..827
                     /label=attB1
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    385..414
                     /label=MET25 promoter?
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#ff8080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1712..1951
                     /label=CYC1 terminator
                     /ApEinfo_fwdcolor=#c0c0c0
                     /ApEinfo_revcolor=#c0c0c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4680..5540
                     /label=AmpR
                     /ApEinfo_fwdcolor=#ffff80
                     /ApEinfo_revcolor=#ffff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(436..692,694..786)
                     /label=MS2 coat protein
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5695..6314
                     /label=pBR322Ori
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2749..3549
                     /label=New Feature
                     /ApEinfo_fwdcolor=#008080
                     /ApEinfo_revcolor=#008080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(841..1064,1066..1551)
                     /label=sfGFP
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    808..808
                     /label=R1
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1615..1617
                     /label=Stop Codon
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1597..1614
                     /label=6xHis
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=#ff80ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    828..832
                     /label=LR1
                     /ApEinfo_fwdcolor=#008000
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    838..840
                     /label=Start Codon(1)
                     /ApEinfo_label=Start Codon
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1660..1678
                     /label=MS2 hairpin
                     /ApEinfo_fwdcolor=#fc293f
                     /ApEinfo_revcolor=#fc293f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1566..1576
                     /label=R2
                     /ApEinfo_fwdcolor=#80ff00
                     /ApEinfo_revcolor=#80ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1634..1659
                     /label=3'UTR
                     /ApEinfo_fwdcolor=#ffd1d1
                     /ApEinfo_revcolor=#ffd1d1
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1556..1565
                     /label=R2(1)
                     /ApEinfo_label=R2
                     /ApEinfo_fwdcolor=#80ff00
                     /ApEinfo_revcolor=#80ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1553..1555
                     /label=LR2
                     /ApEinfo_fwdcolor=#80ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1679..1698
                     /label=3'UTR(1)
                     /ApEinfo_label=3'UTR
                     /ApEinfo_fwdcolor=#ffd1d1
                     /ApEinfo_revcolor=#ffd1d1
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCGGATGCA AGGGTTCGAA TCCCTTAGCT
       61 CTCATTATTT TTTGCTTTTT CTCTTGAGGT CACATGATCG CAAAATGGCA AATGGCACGT
      121 GAAGCTGTCG ATATTGGGGA ACTGTGGTGG TTGGCAAATG ACTAATTAAG TTAGTCAAGG
      181 CGCCATCCTC ATGAAAACTG TGTAACATAA TAACCGAAGT GTCGAAAAGG TGGCACCTTG
      241 TCCAATTGAA CACGCTCGAT GAAAAAAATA AGATATATAT AAGGTTAAGT AAAGCGTCTG
      301 TTAGAAAGGA AGTTTTTCCT TTTTCTTGCT CTCTTGTCTT TTCATCTACT ATTTCCTTCG
      361 TGTAATACAG GGTCGTCAGA TACATAGATA CAATTCTATT ACCCCCATCC ATACTCTAGA
      421 ATGCTAGCCG TTAAAATGGC TTCTAACTTT ACTCAGTTCG TTCTCGTCGA CAATGGCGGA
      481 ACTGGCGACG TGACTGTCGC CCCAAGCAAC TTCGCTAACG GGATCGCTGA ATGGATCAGC
      541 TCTAACTCGC GTTCACAGGC TTACAAAGTA ACCTGTAGCG TTCGTCAGAG CTCTGCGCAG
      601 AATCGCAAAT ACACCATCAA AGTCGAGGTG CCTAAAGGCG CCTGGCGTTC GTACTTAAAT
      661 ATGGAACTAA CCATTCCAAT TTTCGCCACG AATTCCGACT GCGAGCTTAT TGTTAAGGCA
      721 ATGCAAGGTC TCCTAAAAGA TGGAAACCCG ATTCCCTCAG CAATCGCAGC AAACTCCGGC
      781 ATCTACGCGG ATCCACCGGT CGCCACCACA AGTTTGTACA AAAAAGCAGG CTACAAAATG
      841 AGAAAGGGCG AAGAACTGTT TACCGGGGTA GTCCCTATAC TTGTTGAATT GGATGGTGAC
      901 GTTAATGGCC ATAAATTTAG TGTCAGAGGT GAAGGTGAGG GCGACGCTAC AAATGGTAAG
      961 TTGACGTTAA AATTTATATG TACAACGGGA AAATTACCAG TTCCCTGGCC GACACTTGTG
     1021 ACTACTTTAA CATATGGTGT ACAATGTTTC GCACGTTATC CAGACCACAT GAAACAACAC
     1081 GATTTTTTCA AGTCAGCAAT GCCAGAGGGA TATGTACAAG AACGTACCAT TAGCTTCAAA
     1141 GATGACGGTA CGTACAAGAC AAGAGCCGAG GTCAAATTTG AGGGTGATAC ACTAGTGAAC
     1201 AGGATTGAGT TAAAGGGTAT AGATTTTAAA GAAGACGGTA ATATATTAGG TCACAAATTA
     1261 GAATACAACT TTAACAGCCA CAACGTATAC ATCACCGCTG ATAAACAAAA AAATGGCATA
     1321 AAGGCAAACT TTAAAATCAG ACATAATGTT GAAGACGGTA GCGTTCAATT AGCTGATCAC
     1381 TATCAACAAA ATACTCCGAT CGGCGATGGA CCAGTATTAT TACCAGATAA TCATTACCTG
     1441 TCCACCCAAT CCGTCTTGTC TAAGGATCCA AACGAAAAGA GAGATCATAT GGTTTTGTTA
     1501 GAATTTGTTA CTGCAGCCGG CATTACACAC GGCATGGACG AATTATATAA AAACCCAGCT
     1561 TTCTTGTACA AAGTGGTTTC AAGAGGGCCC TTCGAACATC ATCACCATCA CCATTAAATC
     1621 GATACCGTCG ACGATCCTAC GGTACTTATT GCCAAGAAAG CACGAGCATC AGCCGTGCCT
     1681 CCAGGTCGAA TCTTCAAACT CGAGTCATGT AATTAGTTAT GTCACGCTTA CATTCACGCC
     1741 CTCCCCCCAC ATCCGCTCTA ACCGAAAAGG AAGGAGTTAG ACAACCTGAA GTCTAGGTCC
     1801 CTATTTATTT TTTTATAGTT ATGTTAGTAT TAAGAACGTT ATTTATATTT CAAATTTTTC
     1861 TTTTTTTTCT GTACAGACGC GTGTACGCAT GTAACATTAT ACTGAAAACC TTGCTTGAGA
     1921 AGGTTTTGGG ACGCTCGAAG GCTTTAATTT GCGGCCGGTA CCCAATTCGC CCTATAGTGA
     1981 GTCGTATTAC GCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG
     2041 CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA
     2101 AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCGCGA
     2161 CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC
     2221 TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC
     2281 GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG
     2341 TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC
     2401 ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG
     2461 ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA
     2521 AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA
     2581 CGCGAATTTT AACAAAATAT TAACGTTTAC AATTTCCTGA TGCGGTATTT TCTCCTTACG
     2641 CATCTGTGCG GTATTTCACA CCGCATAGGG TAATAACTGA TATAATTAAA TTGAAGCTCT
     2701 AATTTGTGAG TTTAGTATAC ATGCATTTAC TTATAATACA GTTTTTTAGT TTTGCTGGCC
     2761 GCATCTTCTC AAATATGCTT CCCAGCCTGC TTTTCTGTAA CGTTCACCCT CTACCTTAGC
     2821 ATCCCTTCCC TTTGCAAATA GTCCTCTTCC AACAATAATA ATGTCAGATC CTGTAGAGAC
     2881 CACATCATCC ACGGTTCTAT ACTGTTGACC CAATGCGTCT CCCTTGTCAT CTAAACCCAC
     2941 ACCGGGTGTC ATAATCAACC AATCGTAACC TTCATCTCTT CCACCCATGT CTCTTTGAGC
     3001 AATAAAGCCG ATAACAAAAT CTTTGTCGCT CTTCGCAATG TCAACAGTAC CCTTAGTATA
     3061 TTCTCCAGTA GATAGGGAGC CCTTGCATGA CAATTCTGCT AACATCAAAA GGCCTCTAGG
     3121 TTCCTTTGTT ACTTCTTCTG CCGCCTGCTT CAAACCGCTA ACAATACCTG GGCCCACCAC
     3181 ACCGTGTGCA TTCGTAATGT CTGCCCATTC TGCTATTCTG TATACACCCG CAGAGTACTG
     3241 CAATTTGACT GTATTACCAA TGTCAGCAAA TTTTCTGTCT TCGAAGAGTA AAAAATTGTA
     3301 CTTGGCGGAT AATGCCTTTA GCGGCTTAAC TGTGCCCTCC ATGGAAAAAT CAGTCAAGAT
     3361 ATCCACATGT GTTTTTAGTA AACAAATTTT GGGACCTAAT GCTTCAACTA ACTCCAGTAA
     3421 TTCCTTGGTG GTACGAACAT CCAATGAAGC ACACAAGTTT GTTTGCTTTT CGTGCATGAT
     3481 ATTAAATAGC TTGGCAGCAA CAGGACTAGG ATGAGTAGCA GCACGTTCCT TATATGTAGC
     3541 TTTCGACATG ATTTATCTTC GTTTCCTGCA GGTTTTTGTT CTGTGCAGTT GGGTTAAGAA
     3601 TACTGGGCAA TTTCATGTTT CTTCAACACT ACATATGCGT ATATATACCA ATCTAAGTCT
     3661 GTGCTCCTTC CTTCGTTCTT CCTTCTGTTC GGAGATTACC GAATCAAAAA AATTTCAAAG
     3721 AAACCGAAAT CAAAAAAAAG AATAAAAAAA AAATGATGAA TTGAATTGAA AAGCTGTGGT
     3781 ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC
     3841 GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA
     3901 AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG
     3961 CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT
     4021 GGTTTCTTAG GACGGATCGC TTGCCTGTAA CTTACACGCG CCTCGTATCT TTTAATGATG
     4081 GAATAATTTG GGAATTTACT CTGTGTTTAT TTATTTTTAT GTTTTGTATT TGGATTTTAG
     4141 AAAGTAAATA AAGAAGGTAG AAGAGTTACG GAATGAAGAA AAAAAAATAA ACAAAGGTTT
     4201 AAAAAATTTC AACAAAAAGC GTACTTTACA TATATATTTA TTAGACAAGA AAAGCAGATT
     4261 AAATAGATAT ACATTCGATT AACGATAAGT AAAATGTAAA ATCACAGGAT TTTCGTGTGT
     4321 GGTCTTCTAC ACAGACAAGA TGAAACAATT CGGCATTAAT ACCTGAGAGC AGGAAGAGCA
     4381 AGATAAAAGG TAGTATTTGT TGGCGATCCC CCTAGAGTCT TTTACATCTT CGGAAAACAA
     4441 AAACTATTTT TTCTTTAATT TCTTTTTTTA CTTTCTATTT TTAATTTATA TATTTATATT
     4501 AAAAAATTTA AATTATAATT ATTTTTATAG CACGTGATGA AAAGGACCCA GGTGGCACTT
     4561 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT
     4621 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA
     4681 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG
     4741 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC
     4801 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG
     4861 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC
     4921 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG
     4981 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT
     5041 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG
     5101 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG
     5161 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC
     5221 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT
     5281 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT
     5341 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC
     5401 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA
     5461 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT
     5521 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT
     5581 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA
     5641 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
     5701 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
     5761 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
     5821 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG
     5881 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
     5941 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
     6001 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
     6061 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC
     6121 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
     6181 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
     6241 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
     6301 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
     6361 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG
     6421 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG
     6481 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC
     6541 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTACC
     6601 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCCTATG TTGTGTGGAA
     6661 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG
     6721 CAATTA     
//
