LOCUS       pEGFP-N1-hMOG(beta\1)        5480 bp    DNA     circular SYN
            
DEFINITION  Cloning vector pEGFP-N1, complete sequence, enhanced green
            fluorescent protein (egfp) and neomycin phosphotransferase genes,
            complete cds.
ACCESSION   pEGFP-N1-hMOG(beta\1)
VERSION     pEGFP-N1-hMOG(beta\1).0
KEYWORDS    .
SOURCE      Cloning vector pEGFP-N1
  ORGANISM  Cloning vector pEGFP-N1
            .
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Cormack,B.P., Valdivia,R.H. and Falkow,S.
  TITLE     FACS-optimized mutants of the green fluorescent protein (GFP)
  JOURNAL   Gene 173 (1 SPEC NO), 33-38 (1996)
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     pEGFP-N1 complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc.,
                      1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
COMMENT     ORIGDB|GenBank
            This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an
            order call  (415) 424-8222 or (800) 662-2566, extension 1.
            International customers,  please contact your local distributor.
            For technical information, call  (415) 424-8222 or (800) 662-2566,
            extension 3.
            This sequence has been compiled from information in the sequence
            databases,  published literature and other sources, together with
            partial sequences  obtained by CLONTECH. If you suspect there is an
            error in this sequence,  please contact CLONTECH's Technical
            Service Department at (415) 424-8222 or  (800) 662-2566, extension
            3 or E-mail TECH@CLONTECH.COM.
            
            This file is created by Vector NTI
            http://www.lifetechnologies.com/
            VNTAUTHORNAME|Demo User
            VNTOAUTHORNAME|UNKNOWN
FEATURES             Location/Qualifiers
     source          1..5480
                     /label=Source_1
                     /label=Source_1
                     /vntifkey="98"
                     /mol_type="genomic DNA"
                     /original_location="1..4733"
                     /organism="Cloning vector pEGFP-N1"
                     /db_xref="taxon:47945"
                     /db_xref="taxon:47945"
     gene            1426..2145
                     /label=egfp
                     /label=egfp
                     /vntifkey="60"
                     /gene="egfp"
                     /original_location="679..1398"
     CDS             1426..2145
                     /label=EGFP
                     /label=EGFP
                     /vntifkey="4"
                     /gene="egfp"
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced green fluorescent protein"
                     /protein_id="AAB02574.1"
                     /original_location="679..1398"
                     /db_xref="GI:1377912"
     CDS             3376..4170
                     /label=Kan/Neo R
                     /label=Kan/Neo R
                     /vntifkey="4"
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /original_location="2629..3423"
                     /db_xref="GI:1377913"
     polyA_signal    2299..2349
                     /label=SV40 PolyA
                     /label=SV40 PolyA
                     /vntifkey="25"
     rep_origin      2396..2851
                     /label=f1 origin
                     /label=f1 origin
                     /vntifkey="33"
     rep_origin      4755..5398
                     /label=pUC origin
                     /label=pUC origin
                     /vntifkey="33"
     eukaryotic      1..589
                     /label=CMV  promoter
                     /label=CMV  promoter
                     /vntifkey="29"
     CDS             619..1374
                     /label=hMOG
                     /label=hMOG
                     /vntifkey="4"
                     /original_location="896..1636"
     CDS             619..2142
                     /label=hMOG-EGFP
                     /label=hMOG-EGFP
                     /vntifkey="4"
     CDS             619..705
                     /label=leader peptide
                     /label=leader peptide
                     /vntifkey="4"
     CDS             1081..1146
                     /label=TM-domain 1
                     /label=TM-domain 1
                     /vntifkey="4"
     CDS             1252..1311
                     /label=TM-domain 2
                     /label=TM-domain 2
                     /vntifkey="4"
     CDS             1147..1251
                     /label=ic-loop
                     /label=ic-loop
                     /vntifkey="4"
     CDS             736..1050
                     /label=Ig-domain
                     /label=Ig-domain
                     /vntifkey="4"
     CDS             1312..1348
                     /label=loop 2
                     /label=loop 2
                     /vntifkey="4"
     protein_bind    895..909
                     /label=C1q binding
                     /label=C1q binding
                     /vntifkey="31"
     exon            1349..1374
                     /label=beta 1
                     /label=beta 1
                     /vntifkey="61"
     primer          1331..1392
                     /label=hMOG-beta 1
                     /label=hMOG-beta 1
                     /vntifkey="27"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagat ggcaagctta tcaagaccct ctctgcccag ctgcctctgc
      661 tccttcctcc tcctcctcct cctccaagtg tcttccagct atgcagggca gttcagagtg
      721 ataggaccaa gacaccctat ccgggctctg gtcggggatg aagtggaatt gccatgtcgc
      781 atatctcctg ggaagaacgc tacaggcatg gaggtggggt ggtaccgccc ccccttctct
      841 agggtggttc atctctacag aaatggcaag gaccaagatg gagaccaggc acctgaatat
      901 cggggccgga cagagctgct gaaagatgct attggtgagg gaaaggtgac tctcaggatc
      961 cggaatgtaa ggttctcaga tgaaggaggt ttcacctgct tcttccgaga tcattcttac
     1021 caagaggagg cagcaatgga attgaaagta gaagatcctt tctactgggt gagccctgga
     1081 gtgctggttc tcctcgcggt gctgcctgtg ctcctcctgc agatcactgt tggcctcgtc
     1141 ttcctctgcc tgcagtacag actgagagga aaacttcgag cagagataga gaatctccac
     1201 cggacttttg atccccactt tctgagggtg ccctgctgga agataaccct gtttgtaatt
     1261 gtgccggttc ttggaccctt ggttgccttg atcatctgct acaactggct acatcgaaga
     1321 ctagcagggc aattccttga agagctactc ttccacctgg aagccctctc tggccgaatt
     1381 ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagcaagggc
     1441 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1501 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1561 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1621 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1681 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1741 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1801 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1861 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1921 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1981 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2041 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2101 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     2161 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     2221 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     2281 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2341 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt
     2401 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     2461 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     2521 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     2581 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     2641 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     2701 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     2761 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     2821 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     2881 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2941 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     3001 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3061 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     3121 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     3181 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3241 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3301 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     3361 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     3421 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     3481 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     3541 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     3601 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     3661 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     3721 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     3781 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     3841 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     3901 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     3961 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     4021 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     4081 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     4141 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     4201 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     4261 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     4321 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     4381 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     4441 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     4501 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     4561 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     4621 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4681 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     4741 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4801 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4861 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4921 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4981 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5041 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5101 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5161 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5221 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5281 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5341 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5401 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5461 accgtattac cgccatgcat
//
