LOCUS       Exported                5356 bp ds-DNA     circular SYN 25-SEP-2020
DEFINITION  Cloning vector pEGFP-N1, complete sequence, enhanced green 
            fluorescent protein (egfp) and neomycin phosphotransferase genes, 
            complete cds.
ACCESSION   pEGFP-N1-hMOG(beta\3)
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Cormack BP, Valdivia RH, Falkow S.
  TITLE     FACS-optimized mutants of the green fluorescent protein (GFP)
  JOURNAL   Gene 173 (1 SPEC NO), 33-38 (1996)
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Kitts PA.
  TITLE     pEGFP-N1 complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5356)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
REFERENCE   4  (bases 1 to 5356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 25, 2020 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 173 (1
            SPEC NO), 33-38 (1996)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East
            Meadow Circle, Palo Alto, CA 94303-4230, USA"
COMMENT     ORIGDB|GenBank
            This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an 
            order call  (415) 424-8222 or (800) 662-2566, extension 1. 
            International customers,  please contact your local distributor. For
            technical information, call  (415) 424-8222 or (800) 662-2566, 
            extension 3.
            This sequence has been compiled from information in the sequence 
            databases,  published literature and other sources, together with 
            partial sequences  obtained by CLONTECH. If you suspect there is an 
            error in this sequence,  please contact CLONTECH's Technical Service
            Department at (415) 424-8222 or  (800) 662-2566, extension 3 or 
            E-mail TECH@CLONTECH.COM.This file is created by Vector NTI 
            http://www.lifetechnologies.com/
            VNTAUTHORNAME|Demo User
            VNTOAUTHORNAME|UNKNOWN
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=/dnas_title=Exported
                     /note="/dnas_title=Exported"
     misc_feature    1..589
                     /label=CMV  promoter
                     /label=CMV  promoter
                     /label=nonstandard type: eukaryotic
                     /note="/vntifkey=29"
     CDS             612..2021
                     /codon_start=1
                     /label=MOG beta2 EGFP
                     /translation="MASLSRPSLPSCLCSFLLLLLLQVSSSYAGQFRVIGPRHPIRALV
                     GDEVELPCRISPGKNATGMEVGWYRPPFSRVVHLYRNGKDQDGDQAPEYRGRTELLKDA
                     IGEGKVTLRIRNVRFSDEGGFTCFFRDHSYQEEAAMELKVEDPFYWVSPGVLVLLAVLP
                     VLLLQITVGLIFLCLQYRLRGKLRAEIENLHRTFGQFLEELLFHLEALSGRILQSTVPR
                     ARDPPVATMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTT
                     GKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTR
                     AEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIR
                     HNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA
                     GITLGMDELYK"
     CDS             612..1250
                     /codon_start=1
                     /gene="MOG"
                     /gene_synonym="BTN6; BTNL11; MOGIG2; NRCLP7"
                     /product="myelin-oligodendrocyte glycoproteinisoformbeta5 
                     precursor"
                     /label=MOG beta 2
                     /label=MOG
                     /note="isoform beta5 precursor is 
                     encodedbytranscriptvariant beta5; MOG Ig-AluB; MOG alpha-5;
                     MOG AluA; MOG AluB"
                     /db_xref="CCDS:CCDS34367.1"
                     /db_xref="GeneID:4340"
                     /db_xref="HGNC:HGNC:7197"
                     /db_xref="MIM:159465"
                     /protein_id="NP_996533.2"
                     /translation="MASLSRPSLPSCLCSFLLLLLLQVSSSYAGQFRVIGPRHPIRALV
                     GDEVELPCRISPGKNATGMEVGWYRPPFSRVVHLYRNGKDQDGDQAPEYRGRTELLKDA
                     IGEGKVTLRIRNVRFSDEGGFTCFFRDHSYQEEAAMELKVEDPFYWVSPGVLVLLAVLP
                     VLLLQITVGLIFLCLQYRLRGKLRAEIENLHRTFGQFLEELLFHLEALSG"
     gene            1302..2021
                     /gene="egfp"
                     /label=egfp
                     /label=egfp
                     /note="/original_location=679..1398"
                     /note="/vntifkey=60"
     CDS             1302..2021
                     /codon_start=1
                     /transl_table=11
                     /gene="egfp"
                     /product="enhanced green fluorescent protein"
                     /label=EGFP
                     /label=EGFP
                     /note="/original_location=679..1398"
                     /note="/vntifkey=4"
                     /db_xref="GI:1377912"
                     /protein_id="AAB02574.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    2175..2225
                     /label=SV40 PolyA
                     /label=SV40 PolyA
                     /note="/vntifkey=25"
     rep_origin      2272..2727
                     /label=f1 origin
                     /label=f1 origin
                     /note="/vntifkey=33"
     CDS             3252..4046
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /label=Kan/Neo R
                     /label=Kan/Neo R
                     /note="/original_location=2629..3423"
                     /note="/vntifkey=4"
                     /db_xref="GI:1377913"
                     /protein_id="AAB02575.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      4631..5274
                     /label=pUC origin
                     /label=pUC origin
                     /note="/vntifkey=33"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag aatggcaagc ttatcaagac cctctctgcc cagctgcctc tgctccttcc
      661 tcctcctcct cctcctccaa gtgtcttcca gctatgcagg gcagttcaga gtgataggac
      721 caagacaccc tatccgggct ctggtcgggg atgaagtgga attgccatgt cgcatatctc
      781 ctgggaagaa cgctacaggc atggaggtgg ggtggtaccg cccccccttc tctagggtgg
      841 ttcatctcta cagaaatggc aaggaccaag atggagacca ggcacctgaa tatcggggcc
      901 ggacagagct gctgaaagat gctattggtg agggaaaggt gactctcagg atccggaatg
      961 taaggttctc agatgaagga ggtttcacct gcttcttccg agatcattct taccaagagg
     1021 aggcagcaat ggaattgaaa gtagaagatc ctttctactg ggtgagccct ggagtgctgg
     1081 ttctcctcgc ggtgctgcct gtgctcctcc tgcagatcac tgttggcctc atcttcctct
     1141 gcctgcagta cagactgaga ggaaaacttc gagcagagat agagaatctc caccggactt
     1201 ttgggcaatt ccttgaagag ctactcttcc acctggaagc cctctctggc cgaattctgc
     1261 agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagc aagggcgagg
     1321 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1381 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1441 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     1501 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1561 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1621 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1681 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1741 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1801 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1861 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1921 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1981 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagat
     2041 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     2101 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     2161 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     2221 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa
     2281 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2341 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2401 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2461 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2521 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2581 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2641 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2701 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     2761 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2821 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     2881 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     2941 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     3001 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3061 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3121 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3181 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     3241 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     3301 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     3361 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     3421 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     3481 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     3541 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     3601 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     3661 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     3721 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     3781 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     3841 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     3901 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     3961 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     4021 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     4081 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     4141 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     4201 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     4261 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     4321 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     4381 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     4441 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     4501 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4561 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4621 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4681 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4741 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4801 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4861 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4921 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4981 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5041 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5101 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5161 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5221 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5281 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5341 tattaccgcc atgcat
//
