LOCUS       Exported               11433 bp ds-DNA     circular SYN 24-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11433)
  AUTHORS   Arthur
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 24, 2020 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11433
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     primer_bind     2054..2071
                     /label=cPPT_Seq_F
     primer_bind     2184..2234
                     /label=UCOE_F_V2
     misc_feature    2209..2879
                     /label=UCOE
     misc_feature    2209..2449
                     /label=minUCOE
                     /label=nonstandard type:  chromatin
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     misc_feature    complement(2223..2231)
                     /label=CTCF binding site
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     primer_bind     2320..2338
                     /label=UCOE_Colony.FOR
     misc_feature    2398..2411
                     /label=CTCF binding site
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     primer_bind     complement(2660..2676)
                     /label=UCOE_Colony.REV
     mRNA            2807..2879
                     /label=CBX3 mRNA
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
     primer_bind     complement(2856..2905)
                     /label=UCOE_R_V3
     misc_feature    2886..2905
                     /label=origin
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
     misc_feature    2886..2905
                     /label=EFS
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
     promoter        2887..4058
                     /label=EF-1a
     promoter        2899..3110
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(2942..2959)
                     /label=EF1a_begin_seq.REV
     intron          3111..4049
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(3820..3829)
                     /label=mTurq2_Col_F
     intron          3846..4049
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     3856..3875
                     /label=EF-1a_seq.F
     primer_bind     complement(4030..4059)
                     /label=SC344vect_4FPR1_R
     primer_bind     4046..4085
                     /label=Stargazin_SC417.FOR
     misc_feature    4066..4071
                     /label=Kozak
     CDS             4072..5082
                     /codon_start=1
                     /label=Stargazin
                     /translation="MGLFDRGVQMLLTTVGAFAAFSLMTIAVGTDYWLYSRGVCKTKSV
                     SENETSKKNEEVMTHSGLWRTCCLEGNFKGLCKQIDHFPEDADYEADTAEYFLRAVRAS
                     SIFPILSVILLFMGGLCIAASEFYKPVNCRKSSKNSKSLTRHNIILSAGIFFVSAGLSN
                     IIGIIVYISANAGDPSKSDSKKNSYSYGWSFYFGALSFIIAEMVGVLAVHMFIDRHKQL
                     RATARATDYLQASAITRIPSYRYRYQRRSRSSSRSTEPSHSRDASPVGVKGFNTLPSTE
                     ISMYTLSRDPLKAATTPTATYNSDRDNSFLQVHNCIQKDSKDSFHANTANRRTTPV"
     primer_bind     complement(4959..4971)
                     /label=CMVf
     primer_bind     5067..5108
                     /label=mTurq2_Stargazin.FOR
     primer_bind     complement(5069..5106)
                     /label=Stargazin_mTurq2.REV
     CDS             5089..5802
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     5131..5149
                     /label=mTurq2_Col_F
     primer_bind     complement(5155..5172)
                     /label=mTurq2_begin_seq.REV
     primer_bind     5501..5518
                     /label=mTurq2_mid_Seq.FOR
     primer_bind     complement(5598..5616)
                     /label=mTurq2_Col_R
     primer_bind     complement(5782..5812)
                     /label=mTurq2_4iLid_R
     primer_bind     5793..5817
                     /label=iLid-Caax.F
     misc_feature    5824..6261
                     /label=iLID
     primer_bind     5859..5880
                     /label=iLid_Col_F
     primer_bind     6196..6213
                     /label=iLID_end_seq.FOR
     primer_bind     complement(6233..6249)
                     /label=iLid_Col_R
     primer_bind     complement(6238..6290)
                     /label=iLID_MluI_IRES.REV
     primer_bind     6265..6288
                     /label=IRES_MluI.FOR
     misc_feature    6274..6902
                     /label=IRES
     primer_bind     complement(6319..6338)
                     /label=PHGrp1_colony.REV
     primer_bind     complement(6424..6443)
                     /label=IRES_5'-Seq_R
     CDS             6903..7331
                     /codon_start=1
                     /label=BlastR
                     /translation="MVMKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEI
                     ISAVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGM
                     CRELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     primer_bind     complement(7304..7351)
                     /label=BlastR_SC417.REV
     misc_feature    7355..7946
                     /label=WPRE
     misc_feature    7355..7943
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     CDS             complement(7826..7837)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8151..8784
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8913..8929)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8937..8953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8961..8991)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9006..9027
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9315..9900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10071..10931)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10932..11036)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11084..11218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cacatatgcg ggaggtggtc
     2221 cctgcagtta cgccaatgat aacccccgcc agaaaaatct tagtagcctt ccctttttgt
     2281 tttccgtgcc ccaactcggc ggattgactc ggccccttcc ggaaacaccc gaatcaactt
     2341 ctagtcaaat tattgttcac gccgcaatga cccacccctg gcccgcgtct gtggaactga
     2401 cccctggtgt acaggagagt tcgctgctga aagtggtccc aaaggggtac tagtttttaa
     2461 gctcccaact ccccctcccc cagcgtctgg aggattccac accctcgcac cgcaggggcg
     2521 aggaagtggg cggagtccgg ttttggcgcc agccgctgag gctgccaagc agaaaagcca
     2581 ccgctgagga gactccggtc actgtcctcg ccccgcctcc cccttccctc cccttgggga
     2641 ccaccgggcg ccacgccgcg aacggtaagt gccgcggtcg tcggcgcctc cgccctcccc
     2701 ctagggcccc aattcccagc gggcgcggcg cgcggcccct ccccccgccg ggcgcgcgcc
     2761 cgctgccccg cccttcgtgg ccgcccggcg tgggcggtgc cacccctccc cccggcggcc
     2821 ccgcgcgcag ctcccggctc cctccccctt cggatgtggc ttgagctgta ggcgcggaga
     2881 ctcgaggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     2941 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     3001 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     3061 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     3121 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     3181 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     3241 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     3301 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     3361 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     3421 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     3481 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     3541 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     3601 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     3661 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     3721 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     3781 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     3841 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     3901 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     3961 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     4021 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg gatccgcgca aatggggctg
     4081 tttgatcgag gtgttcaaat gcttttaacc accgttggtg ctttcgctgc cttcagcttg
     4141 atgaccatcg ctgtgggaac cgactattgg ctgtactcca gaggggtttg caagaccaaa
     4201 agtgtcagtg agaatgaaac cagcaagaag aacgaggaag ttatgaccca ttccggatta
     4261 tggagaacct gctgcctcga agggaacttc aaaggtctgt gcaagcaaat cgaccacttt
     4321 ccggaagacg cggactacga agctgacacc gcagagtatt tcctccgggc cgtgagggcc
     4381 tcgagtatct tcccgatcct gagtgtgatc ctgcttttca tgggtggcct ctgcatcgca
     4441 gccagcgagt tctacaaacc ggtgaactgc aggaagagct cgaagaattc gaaaagcttg
     4501 acacgccaca acatcatcct gagtgctggc atcttcttcg tgtctgcagg tcttagtaat
     4561 atcatcggga tcatcgtgta tatatcagcc aatgctggag acccctccaa gagtgactcc
     4621 aaaaagaaca gctactccta cggctggtcc ttctacttcg gggccctgtc cttcatcatc
     4681 gccgagatgg tcggggtgct ggccgtgcac atgtttatcg accgccacaa acagctgcgg
     4741 gccacggccc gcgccaccga ctacctccag gcctccgcca tcacccgcat ccccagctac
     4801 cgctaccgct accagcgccg cagccgctcc agctcgcgct ccaccgagcc ctctcactcc
     4861 agagacgcct cgcccgtggg cgtgaagggc ttcaacaccc tgccgtccac ggagatctcc
     4921 atgtacaccc tcagtaggga ccccctgaag gctgccacca cgcccaccgc cacctacaac
     4981 tcggacaggg ataacagctt tctccaggtc cacaactgta tccagaagga cagcaaggac
     5041 tctttccacg ccaacacagc caaccgccgg accacgcccg taggatccgt gagcaagggc
     5101 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     5161 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     5221 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     5281 tcctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     5341 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     5401 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5461 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5521 tactttagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     5581 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     5641 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5701 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5761 accgccgccg ggatcactct cggcatggac gagctgtaca agggtagtgg tagtggggag
     5821 tttctggcaa ccacactgga acggatcgag aaaaatttcg tgattactga tccgagactg
     5881 cctgacaacc caatcatttt tgcgagcgat tccttcctgc agctgacaga atattctcgg
     5941 gaagagatcc tggggcgcaa ttgccgtttt ctgcagggac ccgagacaga ccgtgccact
     6001 gttcggaaaa tcagagatgc tattgacaac cagactgaag tgaccgttca gctgatcaat
     6061 tataccaaga gcggcaagaa gttctggaac gtgttccacc tgcagccgat gcgcgattat
     6121 aagggcgacg tccagtactt cattggcgtg cagctggatg gcaccgaacg tcttcatggc
     6181 gccgctgagc gtgaggcggt ctgcctgatc aaaaagacag cctttcagat tgctgaggca
     6241 gcgaacgacg aaaattactt ttagacgcgt cgggccgcgg taacaattgt taactaactt
     6301 aagctagcaa cggtttccct ctagcgggat caattccgcc ccccccccct aacgttactg
     6361 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     6421 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     6481 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     6541 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     6601 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     6661 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     6721 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     6781 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     6841 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata
     6901 ccatggtcat gaaaacattt aacatttctc aacaagatct agaattagta gaagtagcga
     6961 cagagaagat tacaatgctt tatgaggata ataaacatca tgtgggagcg gcaattcgta
     7021 cgaaaacagg agaaatcatt tcggcagtac atattgaagc gtatatagga cgagtaactg
     7081 tttgtgcaga agccattgcg attggtagtg cagtttcgaa tggacaaaag gattttgaca
     7141 cgattgtagc tgttagacac ccttattctg acgaagtaga tagaagtatt cgagtggtaa
     7201 gtccttgtgg tatgtgtagg gagttgattt cagactatgc accagattgt tttgtgttaa
     7261 tagaaatgaa tggcaagtta gtcaaaacta cgattgaaga actcattcca ctcaaatata
     7321 cccgaaatta acccgggcgg ggcgcgtctg gaacaatcaa cctctggatt acaaaatttg
     7381 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     7441 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     7501 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7561 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7621 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7681 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7741 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     7801 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7861 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7921 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat
     7981 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     8041 caagaggagg aggaggtggg tttttccagt cacacctcag gtacctttaa gaccaatgac
     8101 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct
     8161 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     8221 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     8281 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     8341 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     8401 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     8461 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     8521 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     8581 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8641 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8701 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8761 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     8821 aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag cgttttaccg
     8881 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8941 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     9001 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9061 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9121 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9181 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9241 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9301 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9361 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9421 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9481 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9541 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9601 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9661 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9721 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9781 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9841 gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9901 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9961 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    10021 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    10081 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    10141 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    10201 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    10261 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    10321 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10381 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10441 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10501 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10561 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10621 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10681 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10741 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10801 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10861 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10921 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10981 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    11041 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
    11101 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    11161 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    11221 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    11281 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    11341 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttttt aaagaaattg
    11401 tatttgttaa atatgtacta caaacttagt agt
//
