LOCUS       M02_BB1_23_MS2bind_VP64_SV40NLS      2529 bp    DNA     circular UNA 07-Jan-2015
DEFINITION  old name: BB1_linker_FS2_BsaI_FS3
FEATURES             Location/Qualifiers
     CDS             complement(join(2413..2529,1..702))
                     /label="KanR"
     Vector          join(1579..2529,1..983)
                     /origin="070_BB1_23_L_syn_BsaI_1_v2 (1,966bp
                     circular)Target vector: from BsaI cut at 1015^1016 to
                     BsaI cut at 983^984, 1,934bpOrientation: originalPlus
                     strand 5':   no changePlus strand 3':   no changeMinus
                     strand 5':  no changeMinus strand 3':  no change"
     primer          885..901
                     /label="Seq_BB1_BW (B3_63)"
     CDS             985..1404
                     /label="MS2 binding Protein"
     CDS             985..1578
                     /label="MS2_VP64"
     CDS             988..1008
                     /label="SV40 NLS"
     CDS             1420..1578
                     /label="VP64"
     primer          complement(1708..1725)
                     /label="Seq_BB1_FW (B3_62)"
     oriT            complement(1752..2367)
                     /label="ColE1 origin, high copy number"
ORIGIN
        1 GTTCCATAGG ATGGCAAGAT CCTGGTATCG GTCTGCGATT CCGACTCGTC CAACATCAAT
       61 ACAACCTATT AATTTCCCCT CGTCAAAAAT AAGGTTATCA AGTGAGAAAT CACCATGAGT
      121 GACGACTGAA TCCGGTGAGA ATGGCAAGAG TTTATGCATT TCTTTCCAGA CTTGTTCAAC
      181 AGGCCAGCCA TTACGCTCGT CATCAAAATC ACTCGCACCA ACCAAACCGT TATTCATTCG
      241 TGATTGCGCC TGAGCGAGAC GAAATACGCG ATCGCCGTTA AAAGGACAAT TACAAACAGG
      301 AATCGAATGC AACCGGCGCA GGAACACTGC CAGCGCATCA ACAATATTTT CACCTGAATC
      361 AGGATATTCT TCTAATACCT GGAATGCTGT TTTCCCTGGG ATCGCAGTGG TGAGTAACCA
      421 TGCATCATCA GGAGTACGGA TAAAATGCTT GATGGTCGGA AGAGGCATAA ATTCCGTCAG
      481 CCAGTTTAGC CTGACCATCT CATCTGTAAC ATCATTGGCA ACGCTACCTT TGCCATGTTT
      541 CAGAAACAAC TCTGGCGCAT CGGGCTTCCC ATACAATCGA TAGATTGTCG CACCTGATTG
      601 CCCGACATTA TCGCGAGCCC ATTTATACCC ATATAAATCA GCATCCATGT TGGAATTTAA
      661 TCGCGGCTTC AAGCAAGACG TTTCCCGTTG AATATGGCTC ATTTTAGCTT CCTTAGCTCC
      721 TGAAAATCTC GATAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT TATGGTGAAA
      781 GTTGGAACCT CTTACGTGCC GATCAAGTCA AAAGCCTCCG GTCGGAGGCT TTTGACTTTC
      841 TGCTATGGAG GTCAGGTATG ATTTAAATGG TCAGTATTGA GCCTCAGGAA ACAGCTATGA
      901 CATCAAGCTG ACTAGATAAT CTAGCTGATC GTGGACCGAT CATACGTATA ATGCCGTAAG
      961 ATCACGCGCG AATTCGAAGA CGCCATGCCC AAAAAGAAAA GAAAAGTGGG TAGTATGGCG
     1021 TCTAACTTCA CCCAGTTCGT TCTGGTTGAC AACGGTGGTA CCGGTGACGT TACCGTTGCG
     1081 CCGTCTAACT TCGCGAACGG TGTTGCGGAA TGGATCTCTT CTAACTCTCG TTCTCAGGCG
     1141 TACAAAGTTA CCTGCTCTGT TCGTCAATCC TCCGCGCAGA ACCGTAAATA CACCATCAAA
     1201 GTTGAAGTTC CGAAAGTTGC GACCCAGACC GTTGGTGGTG TTGAACTGCC GGTTGCGGCG
     1261 TGGCGTTCTT ACCTGAACAT GGAACTGACC ATCCCGATCT TCGCGACCAA CTCTGACTGC
     1321 GAACTGATCG TTAAAGCGAT GCAGGGTCTG CTGAAAGACG GTAACCCGAT CCCGTCTGCG
     1381 ATCGCGGCGA ACTCTGGTAT CTACGGCTCC GGACGAGCCG ATGCCCTTGA CGATTTTGac
     1441 ttggacatgt tgggttctga cgctttggat gactttgatc ttgatatgct tggttccgac
     1501 gctctagatg atttcgactt ggatatgctg ggatccgatg ccttggaCGA TTTCGACTTG
     1561 GATATGTTGA TCAACTGAGC TTGCGTCTTC GAATTCGAGG CCGCATATCA TTCAGGACGA
     1621 GCCTCAGACT CCAGCGTAAC TGGACTGCAA TCAACTCACT GGCTCACCTT CCGGTCCACG
     1681 ATCAGCTAGA ATCAAGCTGA CTAGATAAAC TGGCCGTCGT TTTACACGGG TGGGCCTTTC
     1741 TTCGGTAGAA AATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT
     1801 TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA
     1861 CTCTTTTTCC GAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT
     1921 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
     1981 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
     2041 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
     2101 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
     2161 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
     2221 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
     2281 TGTCGGGTTT CGCCACCTCT GACTTGAGCA TCGATTTTTG TGATGCTCGT CAGGGGGGCG
     2341 GAGCCTATGG AAAAACGCCA GCAACGCAGA AAGGCCCACC CGAAGGTGAG CCAGGTGATT
     2401 ACATTTAGGT CCTCATTAGA AAAACTCATC GAGCATCAAG TGAAACTGCA ATTTATTCAT
     2461 ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG GAGAAAACTC
     2521 ACCGAGGCA
//
