LOCUS       Exported                5083 bp ds-DNA     circular SYN 26-MAR-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5083)
  TITLE     Bintu et al., 2016
REFERENCE   2  (bases 1 to 5083)
  TITLE     Bintu et al., 2016
REFERENCE   3  (bases 1 to 5083)
  TITLE     Xu et al., 2009, Donahue et al., 2020
REFERENCE   4  (bases 1 to 5083)
  TITLE     Hansen et al., 2014
REFERENCE   5  (bases 1 to 5083)
  TITLE     Xu et al., 2009, Donahue et al., 2020
REFERENCE   6  (bases 1 to 5083)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   7  (bases 1 to 5083)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   8  (bases 1 to 5083)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   9  (bases 1 to 5083)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   10  (bases 1 to 5083)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   11  (bases 1 to 5083)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   12  (bases 1 to 5083)
  AUTHORS   Leonard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 28, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: ""Unpublished Work""
            SGRef: number: 2; type: ""Unpublished Work""
            SGRef: number: 3; type: ""Unpublished Work""
            SGRef: number: 4; type: ""Unpublished Work""
            SGRef: number: 5; type: ""Unpublished Work""
            SGRef: number: 6; type: ""Unpublished Work""
            SGRef: number: 7; type: ""Unpublished Work""
            SGRef: number: 8; type: ""Unpublished Work""
            SGRef: number: 9; type: ""Unpublished Work""
            SGRef: number: 10; type: ""Unpublished Work""
            SGRef: number: 11; type: ""Unpublished Work""
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    31..55
                     /label=TUPV1 Barcode F
                     /note="TUPV barcode"
                     /citation="[3]"
     misc_feature    99..107
                     /label=ZF1 BS
                     /label=ZF43
                     /note="Binding site for ZF43-8"
                     /citation="[6]"
     misc_feature    114..122
                     /label=ZF1 BS
                     /label=ZF43
                     /note="Binding site for ZF43-8"
                     /citation="[7]"
     misc_feature    129..137
                     /label=ZF1 BS
                     /label=ZF43
                     /note="Binding site for ZF43-8"
                     /citation="[8]"
     misc_feature    144..152
                     /label=ZF1 BS
                     /label=ZF43
                     /note="Binding site for ZF43-8"
                     /citation="[9]"
     misc_feature    159..167
                     /label=ZF1 BS
                     /label=ZF43
                     /note="Binding site for ZF43-8"
                     /citation="[10]"
     misc_feature    174..182
                     /label=ZF1 BS
                     /label=ZF43
                     /note="Binding site for ZF43-8"
                     /citation="[11]"
     promoter        190..214
                     /label=YB_TATA Minimal Promoter
                     /citation="[4]"
     CDS             250..945
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    999..1223
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    1241..1265
                     /label=TUPV1 Barcode R
                     /note="TUPV barcode"
                     /citation="[5]"
     misc_feature    1289..1524
                     /label=cHS4
                     /note="Insulator sequence"
                     /citation="[1]"
     misc_feature    1628..1863
                     /label=cHS4
                     /note="Insulator sequence"
                     /citation="[2]"
     rep_origin      1992..2420
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      2490..2625
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2759..2880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2953..2969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2977..3007)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3022..3043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3331..3916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4090..4947)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4948..5052)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcgaag acggtgccaa ggtcttgaca aacgagtgct agtacaagat
       61 cttgacgtcg acggatcggg agatctgaat tacgatcgga gtgaggataa ttagagtgag
      121 gacgatcgga gtgaggaaaa ttagagtgag gacgatcgga gtgaggataa ttagagtgag
      181 gacgatccct ctagagggta tataatgggg gccactagtc tactaccaga gctcatcgct
      241 agcgccacca tggtgagcga gctgattaag gagaacatgc acatgaagct gtatatggag
      301 ggcaccgtga acaaccacca cttcaagtgt acctccgagg gcgaaggcaa gccctacgag
      361 ggcacccaga ccatgagaat caaggcggtc gagggcggcc ctctcccctt cgccttcgac
      421 atcctggcta ccagcttcat gtacggcagc aaaaccttca tcaaccacac ccagggcatc
      481 cccgacttct ttaagcagtc cttccccgag ggcttcacat gggagagagt caccacatac
      541 gaagatgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
      601 tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
      661 acactcggct gggaggcctc caccgaaacc ctgtaccccg ctgacggcgg cctggaaggc
      721 agagccgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttgaaaacc
      781 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac
      841 aggagactgg aaagaatcaa ggaggccgac aaagaaacct acgtcgagca gcacgaggtg
      901 gctgtggcca gatactgcga cctccctagc aaactggggc acagatgagc ggccgctcga
      961 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     1021 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     1081 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     1141 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagtcaata gcaggcatgc
     1201 tggggatgcg gtgggctcta tggcttctga ggcaggtcga gggtacatgc gccttactcc
     1261 ttgtgagcgc atcgcgataa gctctagagg gacagccccc ccccaaagcc cccagggatg
     1321 taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc
     1381 cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag
     1441 gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct
     1501 ggggggatac ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag
     1561 aacggcctgc gtacggatgg ggctctagct ctagagcttg atatcgaatt cctgcagccc
     1621 cgctagaggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct
     1681 agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc
     1741 gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct
     1801 ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc
     1861 tttaggctga aagagagatt tagaatgaca gaatcataga acggcctggg cgcgcctggt
     1921 ggcgaacgat caacagacgg gagagggcaa aagtcttcgc tgacagcgac cggactctag
     1981 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2041 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2101 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2161 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     2221 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2281 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2341 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2401 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttaggctc aattagtcag
     2461 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     2521 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     2581 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     2641 agctcccggg agcttgtata tccattttcg gaggttgggc ttcggaatcg ttttccggga
     2701 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     2761 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     2821 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     2881 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     2941 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3001 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3061 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3121 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3181 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3241 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3301 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3361 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3421 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3481 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3541 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3601 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3661 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3721 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3781 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3841 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
     3901 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3961 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4021 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4081 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4141 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4201 tggccccagt gctgcaatga taccgcgaga tccacgctca ccggctccag atttatcagc
     4261 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4321 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4381 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4441 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4501 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4561 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4621 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4681 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4741 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4801 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4861 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4921 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4981 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5041 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
