LOCUS       Exported                5052 bp ds-DNA     circular SYN 26-MAR-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5052)
  TITLE     Bintu et al., 2016
REFERENCE   2  (bases 1 to 5052)
  TITLE     Bintu et al., 2016
REFERENCE   3  (bases 1 to 5052)
  TITLE     Xu et al., 2009, Donahue et al., 2020
REFERENCE   4  (bases 1 to 5052)
  TITLE     Hansen et al., 2014
REFERENCE   5  (bases 1 to 5052)
  TITLE     Xu et al., 2009, Donahue et al., 2020
REFERENCE   6  (bases 1 to 5052)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   7  (bases 1 to 5052)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   8  (bases 1 to 5052)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   9  (bases 1 to 5052)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   10  (bases 1 to 5052)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   11  (bases 1 to 5052)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   12  (bases 1 to 5052)
  AUTHORS   Joe Muldoon
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 28, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: ""Unpublished Work""
            SGRef: number: 2; type: ""Unpublished Work""
            SGRef: number: 3; type: ""Unpublished Work""
            SGRef: number: 4; type: ""Unpublished Work""
            SGRef: number: 5; type: ""Unpublished Work""
            SGRef: number: 6; type: ""Unpublished Work""
            SGRef: number: 7; type: ""Unpublished Work""
            SGRef: number: 8; type: ""Unpublished Work""
            SGRef: number: 9; type: ""Unpublished Work""
            SGRef: number: 10; type: ""Unpublished Work""
            SGRef: number: 11; type: ""Unpublished Work""
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    31..55
                     /label=TUPV1 Barcode F
                     /note="TUPV barcode"
                     /citation="[3]"
     misc_feature    68..76
                     /label=ZF2 BS
                     /note="Binding site for ZF37-12"
                     /citation="[6]"
     misc_feature    83..91
                     /label=ZF2 BS
                     /note="Binding site for ZF37-12"
                     /citation="[7]"
     misc_feature    98..106
                     /label=ZF2 BS
                     /note="Binding site for ZF37-12"
                     /citation="[8]"
     misc_feature    113..121
                     /label=ZF2 BS
                     /note="Binding site for ZF37-12"
                     /citation="[9]"
     misc_feature    128..136
                     /label=ZF2 BS
                     /note="Binding site for ZF37-12"
                     /citation="[10]"
     misc_feature    143..151
                     /label=ZF2 BS
                     /note="Binding site for ZF37-12"
                     /citation="[11]"
     promoter        159..183
                     /label=YB_TATA Minimal Promoter
                     /citation="[4]"
     CDS             219..914
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    968..1192
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    1210..1234
                     /label=TUPV1 Barcode R
                     /note="TUPV barcode"
                     /citation="[5]"
     misc_feature    1258..1493
                     /label=cHS4
                     /note="Insulator sequence"
                     /citation="[1]"
     misc_feature    1597..1832
                     /label=cHS4
                     /note="Insulator sequence"
                     /citation="[2]"
     rep_origin      1961..2389
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      2459..2594
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2728..2849
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2922..2938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2946..2976)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2991..3012
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3300..3885)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4059..4916)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4917..5021)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcgaag acggtgccaa ggtcttgaca aacgagtgct agtacaagat
       61 ctgcgaggag gacgtgacgc gagaggacgt gtgagctgag gacgtggact aagaggacgt
      121 gacgcgagag gacgtgtgag ctgaggacgt gaacgctctc tagagggtat ataatggggg
      181 ccactagtct actaccagag ctcatcgcta gcgccaccat ggtgagcgag ctgattaagg
      241 agaacatgca catgaagctg tatatggagg gcaccgtgaa caaccaccac ttcaagtgta
      301 cctccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggcggtcg
      361 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca
      421 aaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccccgagg
      481 gcttcacatg ggagagagtc accacatacg aagatggggg cgtgctgacc gctacccagg
      541 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc
      601 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc accgaaaccc
      661 tgtaccccgc tgacggcggc ctggaaggca gagccgacat ggccctgaag ctcgtgggcg
      721 ggggccacct gatctgcaac ttgaaaacca catacagatc caagaaaccc gctaagaacc
      781 tcaagatgcc cggcgtctac tatgtggaca ggagactgga aagaatcaag gaggccgaca
      841 aagaaaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca
      901 aactggggca cagatgagcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca
      961 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     1021 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     1081 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     1141 gaggattggg aagtcaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     1201 gcaggtcgag ggtacatgcg ccttactcct tgtgagcgca tcgcgataag ctctagaggg
     1261 acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc
     1321 agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag
     1381 cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt
     1441 ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc tttaggctga
     1501 aagagagatt tagaatgaca gaatcataga acggcctgcg tacggatggg gctctagctc
     1561 tagagcttga tatcgaattc ctgcagcccc gctagaggga cagccccccc ccaaagcccc
     1621 cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg
     1681 ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca
     1741 cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc
     1801 agacacctgg ggggatacgg ggaaaaagct ttaggctgaa agagagattt agaatgacag
     1861 aatcatagaa cggcctgggc gcgcctggtg gcgaacgatc aacagacggg agagggcaaa
     1921 agtcttcgct gacagcgacc ggactctagg gggtatcccc acgcgccctg tagcggcgca
     1981 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2041 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2101 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2161 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2221 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2281 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2341 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     2401 atgtgtgtca gttaggctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     2461 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     2521 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     2581 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     2641 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     2701 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     2761 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2821 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     2881 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2941 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3001 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3061 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3121 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3181 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3241 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3301 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3361 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3421 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3481 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3541 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3601 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3661 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3721 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3781 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt
     3841 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3901 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3961 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4021 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4081 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4141 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagat
     4201 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4261 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4321 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4381 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4441 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4501 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4561 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4621 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4681 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4741 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4801 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4861 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4921 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4981 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5041 ccacctgacg tc
//
