LOCUS       Exported                5073 bp ds-DNA     circular SYN 26-MAR-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5073)
  TITLE     Bintu et al., 2016
REFERENCE   2  (bases 1 to 5073)
  TITLE     Bintu et al., 2016
REFERENCE   3  (bases 1 to 5073)
  TITLE     Xu et al., 2009, Donahue et al., 2020
REFERENCE   4  (bases 1 to 5073)
  TITLE     Hansen et al., 2014
REFERENCE   5  (bases 1 to 5073)
  TITLE     Xu et al., 2009, Donahue et al., 2020
REFERENCE   6  (bases 1 to 5073)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   7  (bases 1 to 5073)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   8  (bases 1 to 5073)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   9  (bases 1 to 5073)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   10  (bases 1 to 5073)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   11  (bases 1 to 5073)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   12  (bases 1 to 5073)
  AUTHORS   Joe Muldoon
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 28, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: ""Unpublished Work""
            SGRef: number: 2; type: ""Unpublished Work""
            SGRef: number: 3; type: ""Unpublished Work""
            SGRef: number: 4; type: ""Unpublished Work""
            SGRef: number: 5; type: ""Unpublished Work""
            SGRef: number: 6; type: ""Unpublished Work""
            SGRef: number: 7; type: ""Unpublished Work""
            SGRef: number: 8; type: ""Unpublished Work""
            SGRef: number: 9; type: ""Unpublished Work""
            SGRef: number: 10; type: ""Unpublished Work""
            SGRef: number: 11; type: ""Unpublished Work""
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    31..55
                     /label=TUPV2 Barcode F
                     /note="TUPV barcode"
                     /citation="[3]"
     misc_feature    68..76
                     /label=ZF10 BS
                     /note="Binding site for ZF13-6"
                     /citation="[6]"
     misc_feature    83..91
                     /label=ZF10 BS
                     /note="Binding site for ZF13-6"
                     /citation="[7]"
     misc_feature    98..106
                     /label=ZF10 BS
                     /note="Binding site for ZF13-6"
                     /citation="[8]"
     misc_feature    113..121
                     /label=ZF10 BS
                     /note="Binding site for ZF13-6"
                     /citation="[9]"
     misc_feature    128..136
                     /label=ZF10 BS
                     /note="Binding site for ZF13-6"
                     /citation="[10]"
     misc_feature    143..151
                     /label=ZF10 BS
                     /note="Binding site for ZF13-6"
                     /citation="[11]"
     promoter        159..183
                     /label=YB_TATA Minimal Promoter
                     /citation="[4]"
     CDS             219..935
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    989..1213
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    1231..1255
                     /label=TUPV2 Barcode R
                     /note="TUPV barcode"
                     /citation="[5]"
     misc_feature    1279..1514
                     /label=cHS4
                     /note="Insulator sequence"
                     /citation="[1]"
     misc_feature    1618..1853
                     /label=cHS4
                     /note="Insulator sequence"
                     /citation="[2]"
     rep_origin      1982..2410
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      2480..2615
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2749..2870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2943..2959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2967..2997)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3012..3033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3321..3906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4080..4937)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4938..5042)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcgaag acgggcaaaa ccgatgttga cggactaatc ctgacaagat
       61 ctgcgaggaa gatggtacgc gagaagatgg ttgagctgaa gatggtgact aagaagatgg
      121 tacgcgagaa gatggttgag ctgaagatgg taacgctctc tagagggtat ataatggggg
      181 ccactagtct actaccagag ctcatcgcta gcgccaccat ggtgagcaag ggcgaggagc
      241 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      301 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      361 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg
      421 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      481 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      541 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      601 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
      661 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
      721 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
      781 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc
      841 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      901 ccgggatcac tctcggcatg gacgagctgt acaagtaggc ggccgctcga gtctagaggg
      961 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1021 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1081 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1141 gtggggcagg acagcaaggg ggaggattgg gaagtcaata gcaggcatgc tggggatgcg
     1201 gtgggctcta tggcttctga ggcaggtcga agaactattt cctggctgtt acgcgagcgc
     1261 atcgcgataa gctctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
     1321 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
     1381 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
     1441 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
     1501 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgc
     1561 gtacggatgg ggctctagct ctagagcttg atatcgaatt cctgcagccc cgctagaggg
     1621 acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc
     1681 agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag
     1741 cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt
     1801 ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc tttaggctga
     1861 aagagagatt tagaatgaca gaatcataga acggcctggg cgcgcctggt ggcgaacgat
     1921 caacagacgg gagaggacta aagtcttcgc tgacagcgac cggactctag ggggtatccc
     1981 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     2041 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     2101 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     2161 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     2221 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     2281 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     2341 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     2401 aacgcgaatt aattctgtgg aatgtgtgtc agttaggctc aattagtcag caaccatagt
     2461 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     2521 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     2581 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     2641 agcttgtata tccattttcg gaggttgggc ttcggaatcg ttttccggga cgccggctgg
     2701 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     2761 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2821 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     2881 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     2941 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     3001 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     3061 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     3121 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3181 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3241 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3301 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3361 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3421 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3481 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3541 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3601 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3661 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3721 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3781 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3841 accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3901 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3961 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4021 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4081 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4141 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4201 gctgcaatga taccgcgaga tccacgctca ccggctccag atttatcagc aataaaccag
     4261 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4321 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4381 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4441 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4501 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4561 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4621 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4681 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4741 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4801 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4861 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4921 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4981 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5041 cgcacatttc cccgaaaagt gccacctgac gtc
//
