LOCUS       Exported                4866 bp ds-DNA     circular SYN 26-MAR-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4866)
  TITLE     Bintu et al., 2016
REFERENCE   2  (bases 1 to 4866)
  TITLE     Bintu et al., 2016
REFERENCE   3  (bases 1 to 4866)
  TITLE     Hermann, et al. 2014
REFERENCE   4  (bases 1 to 4866)
  TITLE     Xu et al., 2009, Donahue et al., 2020
REFERENCE   5  (bases 1 to 4866)
  TITLE     Hansen et al., 2014
REFERENCE   6  (bases 1 to 4866)
  TITLE     Xu et al., 2009, Donahue et al., 2020
REFERENCE   7  (bases 1 to 4866)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   8  (bases 1 to 4866)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   9  (bases 1 to 4866)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   10  (bases 1 to 4866)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   11  (bases 1 to 4866)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   12  (bases 1 to 4866)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   13  (bases 1 to 4866)
  TITLE     Khalil et al., 2012, Donahue et al. 2020
REFERENCE   14  (bases 1 to 4866)
  AUTHORS   Joe Muldoon
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 28, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: ""Unpublished Work""
            SGRef: number: 2; type: ""Unpublished Work""
            SGRef: number: 3; type: ""Unpublished Work""
            SGRef: number: 4; type: ""Unpublished Work""
            SGRef: number: 5; type: ""Unpublished Work""
            SGRef: number: 6; type: ""Unpublished Work""
            SGRef: number: 7; type: ""Unpublished Work""
            SGRef: number: 8; type: ""Unpublished Work""
            SGRef: number: 9; type: ""Unpublished Work""
            SGRef: number: 10; type: ""Unpublished Work""
            SGRef: number: 11; type: ""Unpublished Work""
            SGRef: number: 12; type: ""Unpublished Work""
            SGRef: number: 13; type: ""Unpublished Work""
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    31..55
                     /label=TUPV2 Barcode F
                     /note="TUPV barcode"
                     /citation="[4]"
     misc_feature    68..76
                     /label=ZF6 BS
                     /note="Binding site for ZF150-4"
                     /citation="[7]"
     misc_feature    83..91
                     /label=ZF6 BS
                     /note="Binding site for ZF150-4"
                     /citation="[8]"
     misc_feature    98..106
                     /label=ZF6 BS
                     /note="Binding site for ZF150-4"
                     /citation="[9]"
     misc_feature    113..121
                     /label=ZF6 BS
                     /note="Binding site for ZF150-4"
                     /citation="[10]"
     misc_feature    128..136
                     /label=ZF6 BS
                     /note="Binding site for ZF150-4"
                     /citation="[11]"
     misc_feature    143..151
                     /label=ZF6 BS
                     /note="Binding site for ZF150-4"
                     /citation="[12]"
     promoter        159..183
                     /label=YB_TATA Minimal Promoter
                     /citation="[5]"
     CDS             219..332
                     /codon_start=1
                     /product="C-gp41-1 split intein fragment"
                     /label=intC
                     /citation="[3]"
                     /translation="MMLKKILKIEELDERELIDIEVSGNHLFYANDILTHNS"
     CDS             333..389
                     /codon_start=1
                     /label=Linker
                     /translation="GSGGGGSGGGGSGGGGSGT"
     CDS             390..653
                     /codon_start=1
                     /product="Zinc finger 43-8"
                     /label=ZF1
                     /label=ZF43-8
                     /citation="[13]"
                     /translation="ARPGERPFQCRICMRNFSRQDRLDRHTRTHTGEKPFQCRICMRNF
                     SQKEHLAGHLRTHTGEKPFQCRICMRNFSRRDNLNRHLKTHLR"
     CDS             669..689
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             702..728
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    782..1006
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    1024..1048
                     /label=TUPV2 Barcode R
                     /note="TUPV barcode"
                     /citation="[6]"
     misc_feature    1072..1307
                     /label=cHS4
                     /note="Insulator sequence"
                     /citation="[1]"
     misc_feature    1411..1646
                     /label=cHS4
                     /note="Insulator sequence"
                     /citation="[2]"
     rep_origin      1775..2203
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      2273..2408
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2542..2663
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2736..2752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2760..2790)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2805..2826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3114..3699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3873..4730)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4731..4835)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcgaag acgggcaaaa ccgatgttga cggactaatc ctgacaagat
       61 ctgcgaggtg taggggacgc gagtgtaggg gtgagctgtg tagggggact aagtgtaggg
      121 gacgcgagtg taggggtgag ctgtgtaggg gaacgctctc tagagggtat ataatggggg
      181 ccactagtct actaccagag ctcatcgcta gcgccaccat gatgctgaag aagatcctga
      241 agatcgagga gctggacgag agagagctga tcgacatcga ggtgagcggc aaccacctgt
      301 tctacgccaa cgacatcctg acccacaaca gcggatccgg tggtggcggg tctggaggag
      361 gaggtagtgg tggaggtggc tctggtaccg ctagacccgg ggagcgcccc ttccagtgtc
      421 gcatttgcat gcggaacttt tcgcgccagg acaggcttga caggcatacc cgtactcata
      481 ccggtgaaaa accgtttcag tgtcggatct gtatgcgaaa tttctcccag aaggagcact
      541 tggcggggca tctacgtacg cacaccggcg agaagccatt ccaatgccga atatgcatgc
      601 gcaacttcag tcgccgcgac aacctgaacc ggcacctaaa aacccacctg agagaattcg
      661 gctctggccc taagaagaag cgcaaagttg gctctggcat gtacccatac gacgtaccag
      721 attacgctta ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
      781 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
      841 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
      901 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
      961 tgggaagtca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcaggt
     1021 cgaagaacta tttcctggct gttacgcgag cgcatcgcga taagctctag agggacagcc
     1081 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag
     1141 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg
     1201 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct
     1261 gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg ctgaaagaga
     1321 gatttagaat gacagaatca tagaacggcc tgcgtacgga tggggctcta gctctagagc
     1381 ttgatatcga attcctgcag ccccgctaga gggacagccc ccccccaaag cccccaggga
     1441 tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg
     1501 tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga
     1561 aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac
     1621 ctggggggat acggggaaaa agctttaggc tgaaagagag atttagaatg acagaatcat
     1681 agaacggcct gggcgcgcct ggtggcgaac gatcaacaga cgggagagga ctaaagtctt
     1741 cgctgacagc gaccggactc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     1801 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     1861 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     1921 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     1981 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2041 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2101 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2161 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     2221 gtcagttagg ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2281 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2341 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     2401 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggaggttg
     2461 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     2521 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     2581 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     2641 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     2701 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     2761 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     2821 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     2881 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2941 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     3001 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     3061 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     3121 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3181 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3241 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3301 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3361 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3421 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3481 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3541 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3601 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt
     3661 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3721 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3781 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3841 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3901 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3961 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agatccacgc
     4021 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4081 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4141 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4201 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4261 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4321 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4381 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4441 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4501 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4561 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4621 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4681 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4741 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4801 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4861 gacgtc
//
