LOCUS       Exported               12581 bp ds-DNA     circular SYN 26-SEP-2020
DEFINITION  Parkinson's disease-associated G2019S mutant of full-length mouse 
            Lrrk2 containing N-terminal Strep-tag II and C-terminal Myc-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12581)
  AUTHORS   Maik Hintze
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 28, 2020 from SnapGene Viewer 5.1.6
            https://www.snapgene.com
COMMENT     N-terminal StrepII-tag is undetectable by Western blotting. NeoR 
            cassette from pcDNA3.1(+) replaced with PuroR cassette from pPUR. 
            This plasmid can only be propagated in Stbl3 cells.
FEATURES             Location/Qualifiers
     source          1..12581
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             905..8569
                     /codon_start=1
                     /label=tagged full-length Lrrk2(G2019S)
                     /translation="MASWSHPQFEKAGMASGACQGCEEEEEEEALKKLIVRLNNVQEGK
                     QIETLLQLLEDMLVFTYSDRASKLFEDKNFHVPLLIVLDSYMRVASVQQAGWSLLCKLI
                     EVCPGTLQSLIGPQDIGNDWEVLGIHRLILKMLTVHHANVNLSIVGLKALDLLLDSGKL
                     TLLILDEECDIFLLIFDAMHRYSANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYTI
                     LLSTFGSFRRDKEIVYHVLCCLHSLAVTCSNVEVLMSGNVRCYNLVVEAMKAFPTNENI
                     QEVSCSLFQKLTLGNFFNILVLNEVHVFVVKAVRQYPENAALQISALSCLALLTETIFL
                     NQDLEERSETQEQSEEEDSEKLFWLEPCYKALVRHRKDKHVQEAACWALNNLLMYQNSL
                     HEKIGDEDGQFPAHREVMLSMLMHSSSKDVFQAAAHALSTLLEQNVNFRKILLAKGVYL
                     NVLELMQKHAHAPEVAESGCKMLSHLFEGSNPSLDTMAAVVPKILTVMKAHGTSLSVQL
                     EALRAILHFVVPGLLEESREDSQCRPNVLRKQCFRTDIHKLVLVALNRFIGNPGIQKCG
                     LKVISSLAHLPDATETLSLQGAVDSVLHTLQMYPDDQEIQCLGLHLMGCLMTKKNFCIG
                     TGHLLAKILASTLQRFKDVAEVQTTGLQTTLSILELSVSFSKLLVHYSFDVVIFHQMSS
                     SVVEQKDEQFLNLCCKCFAKVAVDDELKNTMLERACDQNNSIMVECLLLLGADANQVKG
                     ATSLIYQVCEKESSPKLVELLLNGGCREQDVRKALTVSIQKGDSQVISLLLRKLALDLA
                     NNSICLGGFGIGKIDPSWLGPLFPDKSSNLRKQTNTGSVLARKVLRYQMRNTLQEGVAS
                     GSDGKFSEDALAKFGEWTFIPDSSMDSVFGQSDDLDSEGSESSFLVKRKSNSISVGEVY
                     RDLALQRCSPNAQRHSNSLGPVFDHEDLLRRKRKILSSDESLRSSRLPSHMRQSDSSSS
                     LASEREHITSLDLSANELKDIDALSQKCCLSSHLEHLTKLELHQNSLTSFPQQLCETLK
                     CLIHLDLHSNKFTSFPSFVLKMPRITNLDASRNDIGPTVVLDPAMKCPSLKQLNLSYNQ
                     LSSIPENLAQVVEKLEQLLLEGNKISGICSPLSLKELKILNLSKNHIPSLPGDFLEACS
                     KVESFSARMNFLAAMPALPSSITSLKLSQNSFTCIPEAIFSLPHLRSLDMSHNNIECLP
                     GPAHWKSLNLRELIFSKNQISTLDFSENPHVWSRVEKLHLSHNKLKEIPPEIGCLENLT
                     SLDVSYNLELRSFPNEMGKLSKIWDLPLDGLHLNFDFKHVGCKAKDIIRFLQQRLKKAV
                     PYNRMKLMIVGNTGSGKTTLLQQLMKMKKPELGMQGATVGIDVRDWSIQIRGKRRKDLV
                     LNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVI
                     LVGTHLDVSDEKQRKACISKITKELLNKRGFPTIRDYHFVNATEESDALAKLRKTIINE
                     SLNFKIRDQPVVGQLIPDCYVELEKIILSERKAVPTEFPVINRKHLLQLVNEHQLQLDE
                     NELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKVMAQILTVKVDGCLKHPKGI
                     ISRRDVEKFLSKKKRFPKNYMMQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPH
                     CENSEIIIRLYEMPYFPMGFWSRLINRLLEISPFMLSGRERALRPNRMYWRQGIYLNWS
                     PEAYCLVGSEVLDNRPESFLKITVPSCRKGCILLGRVVDHIDSLMEEWFPGLLEIDICG
                     EGETLLKKWALYSFNDGEEHQKILLDELMKKAEEGDLLINPDQPRLTIPISQIAPDLIL
                     ADLPRNIMLNNDELEFEEAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQE
                     LVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVA
                     DGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYSIAQYCCRMGIKTSEGTPGF
                     RAPEVARGNVIYNQQADVYSFGLLLHDIWTTGSRIMEGLRFPNEFDELAIQGKLPDPVK
                     EYGCAPWPMVEKLITKCLKENPQERPTSAQVFDILNSAELICLMRHILIPKNIIVECMV
                     ATNLNSKSATLWLGCGNTEKGQLSLFDLNTERYSYEEVADSRILCLALVHLAAEKESWV
                     VCGTQSGALLVINVEEETKRHTLEKMTDSVTCLHCNSLAKQSKQSNFLLVGTADGNLMI
                     FEDKAVKCKGAAPLKTLHIGDVSTPLMCLSESLNSSERHITWGGCGTKVFSFSNDFTIQ
                     KLIETKTNQLFSYAAFSDSNIIALAVDTALYIAKKNSPVVEVWDKKTEKLCELIDCVHF
                     LKEVMVKLNKESKHQLSYSGRVKALCLQKNTALWIGTGGGHILLLDLSTRRVIRTIHNF
                     CDSVRAMATAQLGSLKNVMLVLGYKRKSTEGIQEQKEIQSCLSIWDLNLPHEVQNLEKH
                     IEVRTELADKMRKTSVEGTALEQKLISEEDL"
     primer_bind     complement(6982..7014)
                     /label=FR2976
     primer_bind     6982..7014
                     /label=FR2975
     polyA_signal    8601..8825
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        9006..9335
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             9428..10027
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    10257..10378
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(10829..11414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11585..12445)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatggca agctggagcc acccgcagtt cgaaaaagcc ggcatggcca gtggcgcctg
      961 tcagggctgc gaagaggaag aggaggagga ggctctgaag aagttgatag tcaggctgaa
     1021 taatgtccag gaaggcaagc agatcgagac gttgcttcag ctcctggagg acatgctggt
     1081 gttcacctac tcggaccgcg cctccaagtt atttgaagat aaaaatttcc acgtgcctct
     1141 gttgattgtc ctggactcct acatgagagt tgccagtgta cagcaggcgg ggtggtcact
     1201 tctgtgcaaa ttaatagaag tctgtccagg gacattgcaa agcttaatag gaccccagga
     1261 tattggaaat gattgggaag tccttggtat tcaccggctg attcttaaaa tgttaactgt
     1321 tcatcacgcc aatgtaaacc tgtcaatagt tggactaaaa gccttggatc tcctcctaga
     1381 ttcaggtaaa ctcaccttgc tgatactgga tgaagaatgt gatattttct tgttaatttt
     1441 tgatgccatg cacagatatt cagccaatga tgaagtccaa aaactgggat gcaaagcttt
     1501 acacgtgctt tttgagagag tttccgagga acagctgact gagtttgtgg agaacaaaga
     1561 ttacacgata ctgctgagta cgttcggcag cttcagaagg gacaaggaga ttgtgtacca
     1621 cgtactttgc tgcttgcatt ccctggcggt tacatgcagc aatgtagagg tcctcatgag
     1681 tgggaatgtc cggtgctaca atcttgtggt ggaggccatg aaagccttcc ccaccaatga
     1741 aaacatccaa gaggtgagct gctccttgtt ccagaagctt acattaggta actttttcaa
     1801 catcctggtg ttgaacgaag tgcatgtctt tgtggtgaaa gcggtccgac agtatcctga
     1861 gaacgcagcc ttacagatct ctgcactcag ctgtttagca ctcctcactg agactatttt
     1921 cttaaaccaa gacttggagg aaagaagtga gactcaagag cagagcgaag aggaagacag
     1981 tgagaagctt ttctggctgg aaccctgcta taaagccctg gtgcgccatc gaaaggacaa
     2041 acacgtgcag gaggctgcct gctgggcact aaataacctc cttatgtacc agaacagttt
     2101 gcatgagaag atcggagatg aagatggcca gttccctgcg cacagggaag tgatgctgtc
     2161 tatgctgatg cactcttctt ccaaagatgt cttccaagca gctgcacatg ctctgtccac
     2221 tctcttggaa caaaatgtta atttcaggaa aatcctgctg gcaaaaggag tatacctgaa
     2281 tgtcttggaa ttgatgcaga agcatgccca tgcgcctgag gtggcagaga gtggctgcaa
     2341 gatgctgagt cacctgtttg aaggaagtaa cccttctctg gatacaatgg cagcagtggt
     2401 ccctaaaata ctaacagtga tgaaagccca cggaacgtct ctgtcagtcc agctggaggc
     2461 gctgcgagct atcttgcatt tcgttgtgcc aggactattg gaagaatcca gggaggactc
     2521 tcaatgcaga ccaaatgtgc tcagaaaaca gtgtttcagg actgacatcc acaagctggt
     2581 tctagtcgct ctgaacaggt tcattgggaa tcctgggatt cagaaatgtg gattgaaagt
     2641 aatctcttct ctcgcgcacc ttcctgatgc cacagagaca ttgtccctgc aaggagcagt
     2701 tgactcagtc ctccacacct tacagatgta tccagatgac caagaaattc agtgtctggg
     2761 cttacacctt atgggatgct tgatgacaaa gaagaatttc tgcataggga cagggcacct
     2821 cctggcaaaa attctggctt ccactttgca gcgctttaaa gatgttgctg aggtgcagac
     2881 tacaggatta cagacaaccc tgtcaatact tgagctgtca gtatctttct ccaagctgct
     2941 agtgcactat tcctttgatg tggtgatatt tcatcagatg tcttccagtg ttgtagaaca
     3001 aaaggatgag cagttcctca atctatgttg caaatgcttt gcaaaagtgg ccgtggatga
     3061 tgagctgaaa aacaccatgc tagagagagc ctgcgatcag aataacagca tcatggttga
     3121 atgtttgctc ctcttgggag ctgatgccaa ccaagtgaag ggggcaactt ctttaatcta
     3181 tcaggtatgt gagaaagaga gcagtcctaa attggtggaa ctgttgctta atggtggttg
     3241 tcgtgaacaa gatgtacgga aggccctgac cgtaagcatc caaaagggcg acagccaggt
     3301 catcagcttg ctcctcagga aacttgccct ggacctggcc aacaacagca tttgccttgg
     3361 aggatttggc ataggaaaaa ttgatccttc ttggcttggt cctttatttc cagataagtc
     3421 atccaattta aggaagcaaa caaacacagg atctgtccta gcgaggaaag tgctccggta
     3481 tcagatgaga aacacccttc aagaaggcgt ggcctcaggc agtgacggca agttttctga
     3541 agacgcgctg gcgaaatttg gagaatggac ctttattccc gactcttcta tggacagtgt
     3601 gtttggccag agcgatgatc tggatagcga aggcagcgag agctcatttc tcgtgaagag
     3661 gaagtccaac tcaattagtg taggggaagt ttacagagat ctagctctgc agcgctgctc
     3721 accaaatgct cagaggcatt ccaattcgct gggtcctgtt tttgaccatg aagacttact
     3781 gagacgaaaa agaaaaatac tgtcttcaga tgagtctctc aggtcctcaa ggctgccgtc
     3841 ccatatgagg caatcagata gctcttcttc cctggcttct gagagagaac acatcacgtc
     3901 gttagaccta tctgccaacg aactcaaaga tattgatgct ctgagccaga agtgttgcct
     3961 cagtagccac ctggaacatc tcaccaaact ggaacttcac cagaattcac tcacgagctt
     4021 cccacagcag ctgtgtgaga ctctgaagtg tttgatacac ttggatttgc acagtaacaa
     4081 attcacctca tttccctctt tcgtgttgaa aatgccacgt atcaccaacc tagatgcctc
     4141 tcgaaatgac atcgggccaa cagtagtttt agaccctgcg atgaagtgtc caagcctcaa
     4201 acagttgaat ctgtcctata accagctctc ttcaatccca gagaatcttg cccaagtggt
     4261 ggagaaactt gagcagctcc tactggaagg aaataaaata tccgggattt gctctcccct
     4321 gagcctgaag gaactgaaga ttttaaatct tagtaaaaat cacattccat ccctacctgg
     4381 agattttctt gaggcttgtt caaaagtcga gagtttcagt gctcgcatga attttcttgc
     4441 tgcaatgcct gccttacctt cttccataac gagcttaaaa ttgtctcaga actctttcac
     4501 gtgcattcca gaagcgattt tcagtcttcc gcacttgcgg tccttggata tgagccacaa
     4561 caacattgaa tgtctgccgg gacctgcaca ttggaagtct ctgaacttaa gggaactcat
     4621 ttttagcaag aatcagatca gcaccttaga ctttagtgag aacccacacg tgtggtcaag
     4681 agtagagaaa ctgcatctct ctcataataa actgaaagag attcctccag aaattggctg
     4741 ccttgaaaat ctgacgtctc tcgacgtcag ttacaacttg gaactgaggt ccttcccaaa
     4801 tgaaatgggg aagttgagca agatatggga tcttcccttg gacggactgc atctgaattt
     4861 tgactttaag cacgtaggat gcaaggccaa agacatcata aggtttctac aacaacgtct
     4921 gaaaaaggct gtaccctaca accgaatgaa gctcatgatt gtgggaaata cggggagcgg
     4981 taagaccact ttactgcaac aactcatgaa aatgaagaaa ccagaacttg gcatgcaggg
     5041 tgccacagtc ggcatagacg tgcgagactg gtccatccaa atacggggca aaaggagaaa
     5101 ggacctggtt ctaaacgtgt gggattttgc aggtcgtgag gaattctaca gcactcaccc
     5161 ccacttcatg acccagagag ccctctacct ggctgtctat gatctcagca aggggcaggc
     5221 agaggtggac gccatgaagc cctggctctt caatatcaag gctcgtgcct cttcttcccc
     5281 ggtgattctg gtgggcacac atttggatgt ttctgatgag aagcagcgga aagcgtgcat
     5341 aagcaaaatc acgaaggaac tcctaaataa gcgaggattc cccaccatcc gggactacca
     5401 ctttgtgaat gccaccgagg agtcagatgc gctggcaaag cttcggaaaa ccatcataaa
     5461 tgagagcctt aatttcaaga tccgagatca gcctgtggtt gggcagctaa ttccagattg
     5521 ctacgtagaa ctggagaaaa tcattttatc agagcggaaa gctgtgccga ctgagtttcc
     5581 tgtgattaac cggaaacacc tgttacagct cgtgaacgaa catcagctgc agctggatga
     5641 gaacgagctc ccacacgccg ttcacttcct aaatgagtcg ggagttcttc tgcattttca
     5701 agaccctgcc ctgcagctaa gtgacctgta ctttgtggaa cccaagtggc tttgtaaagt
     5761 catggcacag atcttgacag tgaaggtaga cggctgtctg aaacatccta agggcatcat
     5821 ttcccggaga gatgtggaaa aattcctttc aaagaagaag cgattcccga agaactatat
     5881 gatgcaatac tttaaactat tagaaaaatt tcagatcgca ttgccaatag gggaagaata
     5941 tcttctggtt ccaagcagct tgtctgacca caggccagtg atagagctcc cccactgtga
     6001 gaactctgag atcatcatcc ggctgtacga aatgccgtac tttcccatgg gattttggtc
     6061 aagattgatt aaccgattac ttgaaatctc acccttcatg ctttctggca gagagagagc
     6121 actacgccct aacaggatgt attggcggca aggcatctac ttgaattggt ctccagaagc
     6181 atactgtctg gtaggctctg aagtcttaga caatcgacct gagagtttct tgaaaatcac
     6241 agttccgtct tgtagaaaag gttgtattct tctgggccga gttgtggatc atattgactc
     6301 actcatggaa gaatggtttc ccgggttact ggagattgac atttgtgggg aaggagaaac
     6361 tctgttgaag aaatgggcat tgtacagttt taatgatggt gaagaacatc agaagatctt
     6421 gcttgatgag ttgatgaaga aggctgaaga aggagacctg ttaataaatc cagaccaacc
     6481 aaggctcact attccaatat cccagattgc tccggacttg atcttggctg acctgcctag
     6541 aaatatcatg ttgaacaatg atgagttgga atttgaggaa gcaccagagt ttctcttagg
     6601 cgatggaagt tttggatccg tttatcgagc tgcctacgaa ggagaggaag tggctgtgaa
     6661 gatttttaat aagcacacat ctcttaggct gttaagacaa gagttggtgg tcctttgtca
     6721 ccttcaccac cccagcctga tatccttgct ggcggctggt attcgtcctc ggatgttggt
     6781 aatggagttg gcctccaaag gttccttgga tcgcctgctg cagcaggaca aagccagcct
     6841 caccagaacc ctccagcaca ggatcgcgtt gcatgtggcc gacggcctga ggtatctcca
     6901 ctcagccatg attatttacc gtgacctgaa gccccacaat gtgctgcttt ttaccctgta
     6961 tcccaatgct gccatcattg cgaagattgc ggactactcg atcgcacagt actgctgcag
     7021 gatgggaata aagacatcag agggcacccc agggttccgg gcacctgaag ttgccagggg
     7081 gaatgtcatt tataaccaac aggccgatgt ttattctttt ggcttactac ttcacgatat
     7141 ttggacaact gggagtagga ttatggaggg tttgaggttc ccaaatgagt ttgatgagtt
     7201 agccatacaa gggaagttgc cagatccagt taaagaatat ggctgtgccc catggcctat
     7261 ggttgagaag ttaattacaa agtgtttgaa agaaaatcct caagaaagac ccacttctgc
     7321 ccaggtcttt gacattttga attcggctga attaatttgc ctcatgcgac acattttaat
     7381 acctaagaac atcattgttg aatgcatggt tgccacgaat ctcaatagca agagtgcgac
     7441 tctctggttg ggatgtggga acacagaaaa aggacagctt tccttatttg acttaaacac
     7501 ggaaagatac agctatgagg aagttgctga tagtagaata ctgtgcttgg ctttggtgca
     7561 tctcgctgct gagaaagaga gctgggttgt gtgcgggaca cagtctgggg ctctcctggt
     7621 catcaatgtt gaagaggaga caaagagaca caccctggaa aagatgactg attctgtcac
     7681 ttgtttgcat tgcaattccc ttgccaagca gagcaagcaa agtaactttc ttttggtggg
     7741 aactgctgat ggtaacttaa tgatatttga agataaagcc gttaagtgta aaggagctgc
     7801 ccccttgaag acactacaca taggcgatgt cagtacgccc ctgatgtgcc tgagcgagtc
     7861 cctgaattca tctgaaagac acatcacatg gggagggtgt ggcacaaagg tcttctcctt
     7921 ttccaatgat ttcaccattc agaaactcat cgagacaaaa accaaccagc tgttttctta
     7981 cgcagctttc agcgattcta acatcatagc gctggcagta gacacagccc tgtatattgc
     8041 caagaaaaac agccctgtcg tagaggtgtg ggacaagaaa acagaaaagc tctgtgaatt
     8101 aatagactgt gtgcacttct taaaggaggt gatggtaaaa ctaaacaagg aatcgaaaca
     8161 tcagctgtcc tactctggga gggtgaaggc cctctgcctg cagaagaaca cggctctctg
     8221 gatcggaact ggaggaggcc acatcttact cctggatctt tctactcggc gagttatccg
     8281 caccattcac aatttctgtg attctgtgag agccatggcc acagcacaat taggaagcct
     8341 taagaatgtc atgctggttt tggggtacaa gcggaagagt acagagggta tccaagaaca
     8401 aaaagagata caatcttgtt tgtctatttg ggacctcaat cttccacacg aggtgcaaaa
     8461 tttagaaaaa cacattgaag taagaacaga attagctgat aaaatgagga aaacatctgt
     8521 tgaaggtacc gccctcgagc aaaaactcat ctcagaagag gatctgtagg ggcccgttta
     8581 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     8641 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     8701 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     8761 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     8821 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     8881 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8941 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgctgt
     9001 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9061 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     9121 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     9181 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     9241 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     9301 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tgcatgcctg caggtcggcc
     9361 gccacgaccg gtgccgccac catcccctga cccacgcccc tgacccctca caaggagacg
     9421 accttccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     9481 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     9541 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     9601 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     9661 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     9721 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     9781 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     9841 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     9901 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     9961 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
    10021 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgg
    10081 ctccgaccga agccgacccg ggcggccccg ccgaccccgc acccgccccc gaggcccacc
    10141 gactctagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
    10201 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
    10261 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    10321 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    10381 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    10441 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    10501 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    10561 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    10621 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    10681 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    10741 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    10801 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    10861 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    10921 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    10981 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    11041 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    11101 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    11161 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    11221 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    11281 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    11341 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    11401 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    11461 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    11521 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    11581 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    11641 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    11701 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    11761 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    11821 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    11881 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    11941 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    12001 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    12061 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    12121 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    12181 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    12241 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    12301 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    12361 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    12421 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    12481 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    12541 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
