LOCUS       Exported               10562 bp ds-DNA     circular SYN 26-SEP-2020
DEFINITION  Lrrk2 N-terminal protein-protein interaction and Roc-COR GTPase 
            domains; kinase and C-terminal WD40 domains deleted (2019 bp; 
            AA1871-2540).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10562)
  AUTHORS   Maik Hintze
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 28, 2020 from SnapGene Viewer 5.1.6
            https://www.snapgene.com
COMMENT     Myc tag has not been tested
FEATURES             Location/Qualifiers
     source          1..10562
                     /organism="recombinant plasmid"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="CMV enhancer;CMV enhancer;human cytomegalovirus 
                     immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="CMV promoter;CMV promoter;human cytomegalovirus 
                     (CMV) immediate early promoter"
     CDS             905..6547
                     /codon_start=1
                     /label=tagged Lrrk2 del Kinase
                     /label=tagged full-length Lrrk2
                     /note="tagged full-length Lrrk2;tagged full-length 
                     Lrrk2;full-length Lrrk2 with N-terminal Strep tag and 
                     C-terminal Myc tag."
                     /note="LRRK2 Protein Domains:
                     Armadillo AA 51-657 (nt 1097-2917); ANK AA 676-902 (nt 
                     2972-3652); LRR AA 984-1320 (nt 3896-4906)
                     Roc AA 1328-1511 (nt 4928-5479); COR AA 1535-1878 (nt 
                     5549-6580); Kinase AA 1879-2146 (nt 6581-7384)
                     WD40 AA 2164-2515 (nt 7436-8491)
                     "
                     /translation="MASWSHPQFEKAGMASGACQGCEEEEEEEALKKLIVRLNNVQEGK
                     QIETLLQLLEDMLVFTYSDRASKLFEDKNFHVPLLIVLDSYMRVASVQQAGWSLLCKLI
                     EVCPGTLQSLIGPQDIGNDWEVLGIHRLILKMLTVHHANVNLSIVGLKALDLLLDSGKL
                     TLLILDEECDIFLLIFDAMHRYSANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYTI
                     LLSTFGSFRRDKEIVYHVLCCLHSLAVTCSNVEVLMSGNVRCYNLVVEAMKAFPTNENI
                     QEVSCSLFQKLTLGNFFNILVLNEVHVFVVKAVRQYPENAALQISALSCLALLTETIFL
                     NQDLEERSETQEQSEEEDSEKLFWLEPCYKALVRHRKDKHVQEAACWALNNLLMYQNSL
                     HEKIGDEDGQFPAHREVMLSMLMHSSSKDVFQAAAHALSTLLEQNVNFRKILLAKGVYL
                     NVLELMQKHAHAPEVAESGCKMLSHLFEGSNPSLDTMAAVVPKILTVMKAHGTSLSVQL
                     EALRAILHFVVPGLLEESREDSQCRPNVLRKQCFRTDIHKLVLVALNRFIGNPGIQKCG
                     LKVISSLAHLPDATETLSLQGAVDSVLHTLQMYPDDQEIQCLGLHLMGCLMTKKNFCIG
                     TGHLLAKILASTLQRFKDVAEVQTTGLQTTLSILELSVSFSKLLVHYSFDVVIFHQMSS
                     SVVEQKDEQFLNLCCKCFAKVAVDDELKNTMLERACDQNNSIMVECLLLLGADANQVKG
                     ATSLIYQVCEKESSPKLVELLLNGGCREQDVRKALTVSIQKGDSQVISLLLRKLALDLA
                     NNSICLGGFGIGKIDPSWLGPLFPDKSSNLRKQTNTGSVLARKVLRYQMRNTLQEGVAS
                     GSDGKFSEDALAKFGEWTFIPDSSMDSVFGQSDDLDSEGSESSFLVKRKSNSISVGEVY
                     RDLALQRCSPNAQRHSNSLGPVFDHEDLLRRKRKILSSDESLRSSRLPSHMRQSDSSSS
                     LASEREHITSLDLSANELKDIDALSQKCCLSSHLEHLTKLELHQNSLTSFPQQLCETLK
                     CLIHLDLHSNKFTSFPSFVLKMPRITNLDASRNDIGPTVVLDPAMKCPSLKQLNLSYNQ
                     LSSIPENLAQVVEKLEQLLLEGNKISGICSPLSLKELKILNLSKNHIPSLPGDFLEACS
                     KVESFSARMNFLAAMPALPSSITSLKLSQNSFTCIPEAIFSLPHLRSLDMSHNNIECLP
                     GPAHWKSLNLRELIFSKNQISTLDFSENPHVWSRVEKLHLSHNKLKEIPPEIGCLENLT
                     SLDVSYNLELRSFPNEMGKLSKIWDLPLDGLHLNFDFKHVGCKAKDIIRFLQQRLKKAV
                     PYNRMKLMIVGNTGSGKTTLLQQLMKMKKPELGMQGATVGIDVRDWSIQIRGKRRKDLV
                     LNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVI
                     LVGTHLDVSDEKQRKACISKITKELLNKRGFPTIRDYHFVNATEESDALAKLRKTIINE
                     SLNFKIRDQPVVGQLIPDCYVELEKIILSERKAVPTEFPVINRKHLLQLVNEHQLQLDE
                     NELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKVMAQILTVKVDGCLKHPKGI
                     ISRRDVEKFLSKKKRFPKNYMMQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPH
                     CENSEIIIRLYEMPYFPMGFWSRLINRLLEISPFMLSGRERALRPNRMYWRQGIYLNWS
                     PEAYCLVGSEVLDNRPESFLKITVPSCRKGCILLGRVVDHIDSLMEEWFPGLLEIDICG
                     EGETLLKKWALYSFNDGEEHQKILLDELMKKAEEGDLLINPDQPRLTIPISQIAPVEQK
                     LISEEDL"
     primer_bind     complement(931..957)
                     /label=FR3042
     primer_bind     931..957
                     /label=FR3041
     polyA_signal    6582..6806
                     /label=bGH poly(A) signal
                     /note="bGH poly(A) signal;bGH poly(A) signal;bovine growth 
                     hormone polyadenylation signal"
     promoter        6997..7276
                     /label=SVprom
                     /note="SVprom"
     CDS             7409..8007
                     /codon_start=1
                     /label=puroR
                     /note="puroR"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    8238..8359
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal;SV40 poly(A) signal;SV40 
                     polyadenylation signal"
     rep_origin      complement(8810..9395)
                     /direction=LEFT
                     /label=ori
                     /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9566..10426)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="AmpR;AmpR;confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatggca agctggagcc acccgcagtt cgaaaaagcc ggcatggcca gtggcgcctg
      961 tcagggctgc gaagaggaag aggaggagga ggctctgaag aagttgatag tcaggctgaa
     1021 taatgtccag gaaggcaagc agatcgagac gttgcttcag ctcctggagg acatgctggt
     1081 gttcacctac tcggaccgcg cctccaagtt atttgaagat aaaaatttcc acgtgcctct
     1141 gttgattgtc ctggactcct acatgagagt tgccagtgta cagcaggcgg ggtggtcact
     1201 tctgtgcaaa ttaatagaag tctgtccagg gacattgcaa agcttaatag gaccccagga
     1261 tattggaaat gattgggaag tccttggtat tcaccggctg attcttaaaa tgttaactgt
     1321 tcatcacgcc aatgtaaacc tgtcaatagt tggactaaaa gccttggatc tcctcctaga
     1381 ttcaggtaaa ctcaccttgc tgatactgga tgaagaatgt gatattttct tgttaatttt
     1441 tgatgccatg cacagatatt cagccaatga tgaagtccaa aaactgggat gcaaagcttt
     1501 acacgtgctt tttgagagag tttccgagga acagctgact gagtttgtgg agaacaaaga
     1561 ttacacgata ctgctgagta cgttcggcag cttcagaagg gacaaggaga ttgtgtacca
     1621 cgtactttgc tgcttgcatt ccctggcggt tacatgcagc aatgtagagg tcctcatgag
     1681 tgggaatgtc cggtgctaca atcttgtggt ggaggccatg aaagccttcc ccaccaatga
     1741 aaacatccaa gaggtgagct gctccttgtt ccagaagctt acattaggta actttttcaa
     1801 catcctggtg ttgaacgaag tgcatgtctt tgtggtgaaa gcggtccgac agtatcctga
     1861 gaacgcagcc ttacagatct ctgcactcag ctgtttagca ctcctcactg agactatttt
     1921 cttaaaccaa gacttggagg aaagaagtga gactcaagag cagagcgaag aggaagacag
     1981 tgagaagctt ttctggctgg aaccctgcta taaagccctg gtgcgccatc gaaaggacaa
     2041 acacgtgcag gaggctgcct gctgggcact aaataacctc cttatgtacc agaacagttt
     2101 gcatgagaag atcggagatg aagatggcca gttccctgcg cacagggaag tgatgctgtc
     2161 tatgctgatg cactcttctt ccaaagatgt cttccaagca gctgcacatg ctctgtccac
     2221 tctcttggaa caaaatgtta atttcaggaa aatcctgctg gcaaaaggag tatacctgaa
     2281 tgtcttggaa ttgatgcaga agcatgccca tgcgcctgag gtggcagaga gtggctgcaa
     2341 gatgctgagt cacctgtttg aaggaagtaa cccttctctg gatacaatgg cagcagtggt
     2401 ccctaaaata ctaacagtga tgaaagccca cggaacgtct ctgtcagtcc agctggaggc
     2461 gctgcgagct atcttgcatt tcgttgtgcc aggactattg gaagaatcca gggaggactc
     2521 tcaatgcaga ccaaatgtgc tcagaaaaca gtgtttcagg actgacatcc acaagctggt
     2581 tctagtcgct ctgaacaggt tcattgggaa tcctgggatt cagaaatgtg gattgaaagt
     2641 aatctcttct ctcgcgcacc ttcctgatgc cacagagaca ttgtccctgc aaggagcagt
     2701 tgactcagtc ctccacacct tacagatgta tccagatgac caagaaattc agtgtctggg
     2761 cttacacctt atgggatgct tgatgacaaa gaagaatttc tgcataggga cagggcacct
     2821 cctggcaaaa attctggctt ccactttgca gcgctttaaa gatgttgctg aggtgcagac
     2881 tacaggatta cagacaaccc tgtcaatact tgagctgtca gtatctttct ccaagctgct
     2941 agtgcactat tcctttgatg tggtgatatt tcatcagatg tcttccagtg ttgtagaaca
     3001 aaaggatgag cagttcctca atctatgttg caaatgcttt gcaaaagtgg ccgtggatga
     3061 tgagctgaaa aacaccatgc tagagagagc ctgcgatcag aataacagca tcatggttga
     3121 atgtttgctc ctcttgggag ctgatgccaa ccaagtgaag ggggcaactt ctttaatcta
     3181 tcaggtatgt gagaaagaga gcagtcctaa attggtggaa ctgttgctta atggtggttg
     3241 tcgtgaacaa gatgtacgga aggccctgac cgtaagcatc caaaagggcg acagccaggt
     3301 catcagcttg ctcctcagga aacttgccct ggacctggcc aacaacagca tttgccttgg
     3361 aggatttggc ataggaaaaa ttgatccttc ttggcttggt cctttatttc cagataagtc
     3421 atccaattta aggaagcaaa caaacacagg atctgtccta gcgaggaaag tgctccggta
     3481 tcagatgaga aacacccttc aagaaggcgt ggcctcaggc agtgacggca agttttctga
     3541 agacgcgctg gcgaaatttg gagaatggac ctttattccc gactcttcta tggacagtgt
     3601 gtttggccag agcgatgatc tggatagcga aggcagcgag agctcatttc tcgtgaagag
     3661 gaagtccaac tcaattagtg taggggaagt ttacagagat ctagctctgc agcgctgctc
     3721 accaaatgct cagaggcatt ccaattcgct gggtcctgtt tttgaccatg aagacttact
     3781 gagacgaaaa agaaaaatac tgtcttcaga tgagtctctc aggtcctcaa ggctgccgtc
     3841 ccatatgagg caatcagata gctcttcttc cctggcttct gagagagaac acatcacgtc
     3901 gttagaccta tctgccaacg aactcaaaga tattgatgct ctgagccaga agtgttgcct
     3961 cagtagccac ctggaacatc tcaccaaact ggaacttcac cagaattcac tcacgagctt
     4021 cccacagcag ctgtgtgaga ctctgaagtg tttgatacac ttggatttgc acagtaacaa
     4081 attcacctca tttccctctt tcgtgttgaa aatgccacgt atcaccaacc tagatgcctc
     4141 tcgaaatgac atcgggccaa cagtagtttt agaccctgcg atgaagtgtc caagcctcaa
     4201 acagttgaat ctgtcctata accagctctc ttcaatccca gagaatcttg cccaagtggt
     4261 ggagaaactt gagcagctcc tactggaagg aaataaaata tccgggattt gctctcccct
     4321 gagcctgaag gaactgaaga ttttaaatct tagtaaaaat cacattccat ccctacctgg
     4381 agattttctt gaggcttgtt caaaagtcga gagtttcagt gctcgcatga attttcttgc
     4441 tgcaatgcct gccttacctt cttccataac gagcttaaaa ttgtctcaga actctttcac
     4501 gtgcattcca gaagcgattt tcagtcttcc gcacttgcgg tccttggata tgagccacaa
     4561 caacattgaa tgtctgccgg gacctgcaca ttggaagtct ctgaacttaa gggaactcat
     4621 ttttagcaag aatcagatca gcaccttaga ctttagtgag aacccacacg tgtggtcaag
     4681 agtagagaaa ctgcatctct ctcataataa actgaaagag attcctccag aaattggctg
     4741 ccttgaaaat ctgacgtctc tcgacgtcag ttacaacttg gaactgaggt ccttcccaaa
     4801 tgaaatgggg aagttgagca agatatggga tcttcccttg gacggactgc atctgaattt
     4861 tgactttaag cacgtaggat gcaaggccaa agacatcata aggtttctac aacaacgtct
     4921 gaaaaaggct gtaccctaca accgaatgaa gctcatgatt gtgggaaata cggggagcgg
     4981 taagaccact ttactgcaac aactcatgaa aatgaagaaa ccagaacttg gcatgcaggg
     5041 tgccacagtc ggcatagacg tgcgagactg gtccatccaa atacggggca aaaggagaaa
     5101 ggacctggtt ctaaacgtgt gggattttgc aggtcgtgag gaattctaca gcactcaccc
     5161 ccacttcatg acccagagag ccctctacct ggctgtctat gatctcagca aggggcaggc
     5221 agaggtggac gccatgaagc cctggctctt caatatcaag gctcgtgcct cttcttcccc
     5281 ggtgattctg gtgggcacac atttggatgt ttctgatgag aagcagcgga aagcgtgcat
     5341 aagcaaaatc acgaaggaac tcctaaataa gcgaggattc cccaccatcc gggactacca
     5401 ctttgtgaat gccaccgagg agtcagatgc gctggcaaag cttcggaaaa ccatcataaa
     5461 tgagagcctt aatttcaaga tccgagatca gcctgtggtt gggcagctaa ttccagattg
     5521 ctacgtagaa ctggagaaaa tcattttatc agagcggaaa gctgtgccga ctgagtttcc
     5581 tgtgattaac cggaaacacc tgttacagct cgtgaacgaa catcagctgc agctggatga
     5641 gaacgagctc ccacacgccg ttcacttcct aaatgagtcg ggagttcttc tgcattttca
     5701 agaccctgcc ctgcagctaa gtgacctgta ctttgtggaa cccaagtggc tttgtaaagt
     5761 catggcacag atcttgacag tgaaggtaga cggctgtctg aaacatccta agggcatcat
     5821 ttcccggaga gatgtggaaa aattcctttc aaagaagaag cgattcccga agaactatat
     5881 gatgcaatac tttaaactat tagaaaaatt tcagatcgca ttgccaatag gggaagaata
     5941 tcttctggtt ccaagcagct tgtctgacca caggccagtg atagagctcc cccactgtga
     6001 gaactctgag atcatcatcc ggctgtacga aatgccgtac tttcccatgg gattttggtc
     6061 aagattgatt aaccgattac ttgaaatctc acccttcatg ctttctggca gagagagagc
     6121 actacgccct aacaggatgt attggcggca aggcatctac ttgaattggt ctccagaagc
     6181 atactgtctg gtaggctctg aagtcttaga caatcgacct gagagtttct tgaaaatcac
     6241 agttccgtct tgtagaaaag gttgtattct tctgggccga gttgtggatc atattgactc
     6301 actcatggaa gaatggtttc ccgggttact ggagattgac atttgtgggg aaggagaaac
     6361 tctgttgaag aaatgggcat tgtacagttt taatgatggt gaagaacatc agaagatctt
     6421 gcttgatgag ttgatgaaga aggctgaaga aggagacctg ttaataaatc cagaccaacc
     6481 aaggctcact attccaatat cccagattgc tccggtcgag caaaaactca tctcagaaga
     6541 ggatctgtag gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     6601 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6661 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6721 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6781 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     6841 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6901 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6961 ctttctcgcc acgttcgctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     7021 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     7081 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7141 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7201 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7261 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     7321 ttgcatgcct gcaggtcggc cgccacgacc ggtgccgcca ccatcccctg acccacgccc
     7381 ctgacccctc acaaggagac gaccttccat gaccgagtac aagcccacgg tgcgcctcgc
     7441 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     7501 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
     7561 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     7621 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     7681 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     7741 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     7801 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     7861 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     7921 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     7981 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     8041 aaggagcgca cgaccccatg gctccgaccg aagccgaccc gggcggcccc gccgaccccg
     8101 cacccgcccc cgaggcccac cgactctaga ggatcataat cagccatacc acatttgtag
     8161 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     8221 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8281 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8341 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     8401 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8461 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8521 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8581 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8641 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8701 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8761 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8821 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8881 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8941 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9001 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9061 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9121 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9181 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9241 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9301 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca
     9361 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9421 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9481 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9541 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9601 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9661 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9721 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9781 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9841 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9901 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9961 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10021 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10081 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10141 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10201 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10261 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10321 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10381 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10441 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10501 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10561 tc
//
