LOCUS       pCfB9356(IV-1_Markerfree_BackBone)      4321 bp ds-DNA     circular SYN 02-SEP-2015
DEFINITION  synthetic circular DNA
ACCESSION   BGHA
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct
            synthetic DNA construct
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 28, 2019 from SnapGene Viewer 4.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    1..523
                     /label="IV-1_UP"
                     /label="IV-1_UP"
     terminator      complement(537..731)
                     /label="TADH1"
     terminator      761..950
                     /label="TCYC1"
     misc_feature    959..1474
                     /label="IV-1_DW"
                     /label="IV-1_DW"
     gene            1932..2790
                     /label="AmpR"
     rep_origin      2893..3536
                     /label="pUC ori"
ORIGIN
        1 ATCTCTCCAA GGTTAGCCGC CCATATAAAA AACTCTAGGA ACCAAATTTG GTATACTGCG
       61 TGCGCTTGAG ATTCAGTTTT TGTGTAAATC GGTTGCTGCA AACTATTTAT ATTAATGTTA
      121 CATCGATGCA AGATTTTAGC CATGTTCCTA GTTTCTTGTC TATGAGCGTT TACTCAACCG
      181 AGACTCTTGT TTGCTATTAT CTCGATTCGA CGAACAAACA GTTCCCTGGG TATTCGAACA
      241 AAGCACAGTT GGTGATTTTG TTTGAAACTA CAGACGTGCT CTCTTTTACA AAATAAATTG
      301 TAGGAAGTGA TCCGCACAGG CAGAGGGAAT TACAGATCAC GCTCCTCTCT TATGACTAAT
      361 GCACTCAGCA TTTTCTCTTC CTTATAAACT CCGTCCACAG TACCCGATCA GCTGGCTCAT
      421 CGTTATTCTA AATTAACTGA TGACTGATGT TACGTCTGCG TCAAATTCCG CCGGAATATT
      481 GCGTAGTTTT AGTAAGAAAA AAACAAATTC CGACGGGAAC TCTACGTGCA ACGCTTGAGC
      541 GACCTCATGC TATACCTGAG AAAGCAACCT GACCTACAGG AAAGAGTTAC TCAAGAATAA
      601 GAATTTTCGT TTTAAAACCT AAGAGTCACT TTAAAATTTG TATACACTTA TTTTTTTTAT
      661 AACTTATTTA ATAATAAAAA TCATAAATCA TAAGAAATTC GCTTATTTAG AAGTGTCAAC
      721 AACGTATCTA CCAACGGAAT GCGTGCGATC GCGTGCATTC ATCCGCTCTA ACCGAAAAGG
      781 AAGGAGTTAG ACAACCTGAA GTCTAGGTCC CTATTTATTT TTTTATAGTT ATGTTAGTAT
      841 TAAGAACGTT ATTTATATTT CAAATTTTTC TTTTTTTTCT GTACAGACGC GTGTACGCAT
      901 GTAACATTAT ACTGAAAACC TTGCTTGAGA AGGTTTTGGG ACGCTCGAAG AGTGGCCTTT
      961 ACTTTGCTAG GTTGAGAAAA AGAATTCTTT AGAGGATCAC ACGTAGTAAA AGAAAAAAAA
     1021 GATCTCTTCG CGCCACTCCC GGTTTCTCTG TGGATACGGC GTTACTAGCG TTGCAAGTGG
     1081 CAAAAAAATG AGTAATGTCA CAATGGCCTG GCCTTGTCCC ACTATCCCTT ATTCATGGTT
     1141 GAAAAATATG ACCAAAATTG CTGGGGAATT TTCTCTTCGG TAGTGGACGA CGACGACATT
     1201 AGAGCAATAG GCAATAATTT AATTTGCGCT TTAGGTTCTT GCTGACGATG ATGCGTGAGT
     1261 AATATTATTA CCAAGTTTTA AGGGCCTCAT CGCTGGCCAT TTTTTACACC TGCTTAAATA
     1321 CCCGATGATG ATATATATAA ATGATATTGG CTGCTTATCG ATAAAGTGTA TTTGATATGT
     1381 TCCCGTTTAG TTTATTCTTG TTTCTTATCT ACATAACAAA CAAAGGAAGG ACCCTGTATT
     1441 GTTTTCCTAA AGATGACTGC TGCTAAACCA AATCATCGCG TGCATTCGCG GCCGCATTTA
     1501 AATCCCCACT TCAGAAGTTC CTATACTTTC TAGAGAATAG GAACTTCACT ATAGAGTCGA
     1561 ATAAGGGCGA CACCCCATAA TTAGCCCGGG CGAAAGGCCC AGTCTTTCGA CTGAGCCTTT
     1621 CGTTTTATTT GATGCCTGGC AGTTCCCTAC TCTCGCATGG GGAGTCCCCA CACTACCATC
     1681 GGCGCTACGG CGTTTCACTT CTGAGTTCGG CATGGGGTCA GGTGGGACCA CCGCGCTACT
     1741 GCCGCCAGGC AAACAAGGGG TGTTATGAGC CATATTCAGG TATAAATGGG CTCGCGATAA
     1801 TGTTCAGAAT TGGTTAATTG GTTGTAACAC TGACCCCTAT TTGTTTATTT TTCTAAATAC
     1861 ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA
     1921 AAAGGAAGAA TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT
     1981 TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC
     2041 AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA
     2101 GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG
     2161 CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC
     2221 AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG
     2281 TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC
     2341 TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG
     2401 TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG
     2461 ACACCACGAT GCCTGTAGCG ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC
     2521 TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC
     2581 CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCC GGAGCCGGTG
     2641 AGCGTGGTTC TCGCGGTATC ATCGCAGCGC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG
     2701 TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG
     2761 AGATAGGTGC CTCACTGATT AAGCATTGGT AAAAGCAGAG CATTACGCTG ACTTGACGGG
     2821 ACGGCGCAAG CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGCGCGTC GTTCCACTGA
     2881 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA
     2941 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA
     3001 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT
     3061 GTTCTTCTAG TGTAGCCGTA GTTAGCCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA
     3121 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT
     3181 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG
     3241 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG
     3301 CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA
     3361 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT
     3421 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG
     3481 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC
     3541 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
     3601 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC
     3661 GAGTCAGTGA GCGAGGAAGC GGAAGGCGAG AGTAGGGAAC TGCCAGGCAT CAAACTAAGC
     3721 AGAAGGCCCC TGACGGATGG CCTTTTTGCG TTTCTACAAA CTCTTTCTGT GTTGTAAAAC
     3781 GACGGCCAGT CTTAAGCTCG GGCCCCCTGG GCGGTTCTGA TAACGAGTAA TCGTTAATCC
     3841 GCAAATAACG TAAAAACCCG CTTCGGCGGG TTTTTTTATG GGGGGAGTTT AGGGAAAGAG
     3901 CATTTGTCAG AATATTTAAG GGCGCCTGTC ACTTTGCTTG ATATATGAGA ATTATTTAAC
     3961 CTTATAAATG AGAAAAAAGC AACGCACTTT AAATAAGATA CGTTGCTTTT TCGATTGATG
     4021 AACACCTATA ATTAAACTAT TCATCTATTA TTTATGATTT TTTGTATATA CAATATTTCT
     4081 AGTTTGTTAA AGAGAATTAA GAAAATAAAT CTCGAAAATA ATAAAGGGAA AATCAGTTTT
     4141 TGATATCAAA ATTATACATG TCAACGATAA TACAAAATAT AATACAAACT ATAAGATGTT
     4201 ATCAGTATTT ATTATGCATT TAGAATAAAT TTTGTGTCGC CCTTATTCGA CTCACTATAG
     4261 AAGTTCCTAT TCTCTAGAAA GTATAGGAAC TTCACTTCAT TTTATTTAAA TTTGCGGCCG
     4321 C
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