LOCUS       pCfB9361(XIII-1_Markerfree_BackBone)      4353 bp ds-DNA     circular SYN 02-SEP-2015
DEFINITION  synthetic circular DNA
ACCESSION   BGHA
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct
            synthetic DNA construct
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 28, 2019 from SnapGene Viewer 4.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     misc_feature    1..537
                     /label="XIII-1 UP"
                     /label="XIII-1 UP"
                     /label="XIII-1_UP"
     terminator      complement(551..745)
                     /label="TADH1"
     terminator      775..964
                     /label="TCYC1"
     misc_feature    973..1506
                     /label="XIII-1 Down"
                     /label="XIII-1 Down"
                     /label="XIII-1_DW"
     gene            1964..2822
                     /label="AmpR"
     rep_origin      2925..3568
                     /label="pUC ori"
ORIGIN
        1 TCCTTCTACT GCGGTTTCCC TGCAGGCACA GCTAACCGGA GGGAAGACAA GTCAAGCGGA
       61 CATGCAGTTC CCGCTCAGGC TTCTTTGCAG AGAAATCGGA ATATGAATTT TTTGTGTTTT
      121 TTGTTAACTT CTTTACCATT CGCATCACGG GAAGAGTGTG TTGGCATAAG AGGCGCAAGA
      181 AAAAGGTGAC CCACGGTGGT GGGAAAGAGA AGAAAAAGCC GTTTGCGGAA AGCAATGTTT
      241 TGCCCATTCA GAAACAATAT CTTTGCTGTA TCCCTACATG TATTATGCCG CGTTGCTTGT
      301 GCAGGTACGG AGTCCCTGCA CGATTTTTCA ACAAGGTCTA GATTTGCTAA GACGATGAGG
      361 CGGAGCCCGA AACAAACAAG CCAAAAACCG GAATTTCGCG CTTGGTGTGC TTTCGCATCT
      421 TTTCGTGTGG TGAATGCACC TGGTGCACGC TGCGAAGGTG ACGTAGCAAT CCAAAAAAGG
      481 GAAGTCTAGG CGCTTTCAAC CGCCCCCCAC CGTGCATGCG GATTGGTTGT CACAATTACG
      541 TGCAACGCTT GAGCGACCTC ATGCTATACC TGAGAAAGCA ACCTGACCTA CAGGAAAGAG
      601 TTACTCAAGA ATAAGAATTT TCGTTTTAAA ACCTAAGAGT CACTTTAAAA TTTGTATACA
      661 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT
      721 TTAGAAGTGT CAACAACGTA TCTACCAACG GAATGCGTGC GATCGCGTGC ATTCATCCGC
      781 TCTAACCGAA AAGGAAGGAG TTAGACAACC TGAAGTCTAG GTCCCTATTT ATTTTTTTAT
      841 AGTTATGTTA GTATTAAGAA CGTTATTTAT ATTTCAAATT TTTCTTTTTT TTCTGTACAG
      901 ACGCGTGTAC GCATGTAACA TTATACTGAA AACCTTGCTT GAGAAGGTTT TGGGACGCTC
      961 GAAGAGTGGC CTCAATGTTT CCTCCCCTCC TGTTGATGCG GGTAAGGGGT GTCTGTTATG
     1021 CAGGAACAGG AAGGTAAAAA TTAAATGGGC CTTAATTCTG TAGACACTCT CTTGGGGTAA
     1081 TAAGGTTAAA AGCCATTGTT GAAGTACGGA ATCATCTCTT CATTTTTTCA GCGCTTGTAA
     1141 GGAATTAATC CCCAGGAAGG TGCCGATGTA ATGAGAGAAG TATTCTTGTT GTATCTCTTT
     1201 GGTTCGGAGT ATTTCCTTCT TTCTTTTCTT TGTAGTTTTT ATGCAAATGC AGGGAACAGA
     1261 GTACCCGGTT AAGGCTGTTA GTCAGCCGCA CTCTACACAA AATTACTTGG CAAACACGTT
     1321 GGTTGGGAAA AGCTTTATAC TTGGCGGTGT AGTAATTGTA AAGGATCAAG ATTGTGTCAT
     1381 GCTAATGTGC CGCTGTGTAA CAACCTTTTT GAAGCGCGTA TTTACCACCG GCAAAAGGCC
     1441 ATTGTGTCAC ATAAAAAAAA ACTCCCGCCA AAGCCAGGTT CATTTTGCAT AAGATTCACC
     1501 ACAGTAATCG CGTGCATTCG CGGCCGCATT TAAATCCCCA CTTCAGAAGT TCCTATACTT
     1561 TCTAGAGAAT AGGAACTTCA CTATAGAGTC GAATAAGGGC GACACCCCAT AATTAGCCCG
     1621 GGCGAAAGGC CCAGTCTTTC GACTGAGCCT TTCGTTTTAT TTGATGCCTG GCAGTTCCCT
     1681 ACTCTCGCAT GGGGAGTCCC CACACTACCA TCGGCGCTAC GGCGTTTCAC TTCTGAGTTC
     1741 GGCATGGGGT CAGGTGGGAC CACCGCGCTA CTGCCGCCAG GCAAACAAGG GGTGTTATGA
     1801 GCCATATTCA GGTATAAATG GGCTCGCGAT AATGTTCAGA ATTGGTTAAT TGGTTGTAAC
     1861 ACTGACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA
     1921 ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AATATGAGTA TTCAACATTT
     1981 CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA
     2041 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA
     2101 ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT
     2161 GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA
     2221 AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT
     2281 CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC
     2341 CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT
     2401 AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA
     2461 GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CGATGGCAAC
     2521 AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT
     2581 AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG
     2641 CTGGTTTATT GCTGATAAAT CCGGAGCCGG TGAGCGTGGT TCTCGCGGTA TCATCGCAGC
     2701 GCTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC
     2761 AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG
     2821 GTAAAAGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT
     2881 TAACGTGAGT TACGCGCGCG TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG
     2941 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC
     3001 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA
     3061 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGCCC
     3121 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG
     3181 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC
     3241 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC
     3301 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC
     3361 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA
     3421 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC
     3481 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG
     3541 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT
     3601 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA
     3661 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGGCG
     3721 AGAGTAGGGA ACTGCCAGGC ATCAAACTAA GCAGAAGGCC CCTGACGGAT GGCCTTTTTG
     3781 CGTTTCTACA AACTCTTTCT GTGTTGTAAA ACGACGGCCA GTCTTAAGCT CGGGCCCCCT
     3841 GGGCGGTTCT GATAACGAGT AATCGTTAAT CCGCAAATAA CGTAAAAACC CGCTTCGGCG
     3901 GGTTTTTTTA TGGGGGGAGT TTAGGGAAAG AGCATTTGTC AGAATATTTA AGGGCGCCTG
     3961 TCACTTTGCT TGATATATGA GAATTATTTA ACCTTATAAA TGAGAAAAAA GCAACGCACT
     4021 TTAAATAAGA TACGTTGCTT TTTCGATTGA TGAACACCTA TAATTAAACT ATTCATCTAT
     4081 TATTTATGAT TTTTTGTATA TACAATATTT CTAGTTTGTT AAAGAGAATT AAGAAAATAA
     4141 ATCTCGAAAA TAATAAAGGG AAAATCAGTT TTTGATATCA AAATTATACA TGTCAACGAT
     4201 AATACAAAAT ATAATACAAA CTATAAGATG TTATCAGTAT TTATTATGCA TTTAGAATAA
     4261 ATTTTGTGTC GCCCTTATTC GACTCACTAT AGAAGTTCCT ATTCTCTAGA AAGTATAGGA
     4321 ACTTCACTTC ATTTTATTTA AATTTGCGGC CGC
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