LOCUS       Exported                8450 bp ds-DNA     circular SYN 09-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 12, 2020 from SnapGene Viewer 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    322..442
                     /label=SV40-PA terminator
     misc_feature    1068..1256
                     /label=5' LTR
     misc_feature    1307..1444
                     /label=Psi
     misc_feature    1411..1764
                     /label=GAG
     misc_feature    1918..2216
                     /label=PRE
     misc_feature    2217..2633
                     /label=ENV
     misc_feature    2648..2765
                     /label=CPPT
     promoter        2813..3397
                     /label=CMV promoter (pLVSIN)
     primer_bind     complement(2924..2945)
                     /label=NM-MB-CAGp-check-Rv #418 #NM74
     primer_bind     3297..3316
                     /label=CMVp-QC-FW #471
     primer_bind     3404..3448
                     /label=pLVSIN-EcoR1-EGFP-FW #649
     gene            3430..4149
                     /label=EGFP
     primer_bind     3430..3454
                     /label=ATG-FFP-FW #562
     primer_bind     complement(3603..3627)
                     /label=EGFP-N-RV #534
     primer_bind     3926..3945
                     /label=EGFP-seq-FW #116
     misc_feature    4177..4190
                     /label=cloning site
     misc_feature    4234..4291
                     /label=Synthetic poly(A)
     misc_feature    4292..4296
                     /label=MCS
     promoter        4306..4805
                     /label=PGK promoter
     primer_bind     complement(4334..4353)
                     /label=PGKp-QC-FW #420
     misc_feature    4825..5424
                     /label=PuroR
     misc_feature    5436..6029
                     /label=WPRE
     misc_feature    6227..6267
                     /label=Delta-U3
     misc_feature    6268..6467
                     /label=3' LTR
     primer_bind     complement(6591..6611)
                     /label=M13-rev
     misc_binding    complement(6617..6639)
                     /label=LacO
     promoter        complement(6643..6673)
                     /label=lac promoter
     rep_origin      complement(6979..7661)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(7759..8418)
                     /label=AmpR
ORIGIN
        1 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
       61 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
      121 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
      181 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
      241 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      301 tcgacggatc gggagatcaa ctttgtttat tgcagcttat aatggttaca aataaagcaa
      361 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
      421 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
      481 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
      541 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
      601 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
      661 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
      721 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
      781 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
      841 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
      901 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
      961 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     1021 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     1081 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     1141 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     1201 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1261 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1321 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1381 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1441 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1501 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1561 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1621 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1681 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1741 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1801 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1861 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1921 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1981 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     2041 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     2101 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     2161 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     2221 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2281 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2341 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2401 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2461 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2521 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2581 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     2641 gtatcgcctt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     2701 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     2761 attttcgggt ttattacagg gacagcagag atccagttta tcgataagct tgggagttcc
     2821 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     2881 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     2941 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     3001 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     3061 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     3121 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     3181 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     3241 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     3301 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
     3361 gccatccacg ctgttttgac ctccatagaa gacaccgact ctactagagg atctatttcc
     3421 ggtgaattca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     3481 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     3541 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     3601 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     3661 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     3721 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     3781 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     3841 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3901 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3961 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4021 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     4081 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     4141 tacaagtaat aactataacg gtcctaaggt agcgaatcta gatgggatcc attaccctgt
     4201 tatccctact cgaactcgca tacctctgat aacaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4261 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa agatccaatt ctaccgggta ggggaggcgc
     4321 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     4381 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     4441 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     4501 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     4561 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     4621 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     4681 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     4741 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     4801 tttcgacctg cagttaacgc cgccatgacc gagtacaagc ccacggtgcg cctcgccacc
     4861 cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4921 acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4981 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     5041 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     5101 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     5161 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     5221 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     5281 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5341 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5401 atgacccgca agcccggtgc ctagacgcgt ctggaacaat caacctctgg attacaaaat
     5461 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5521 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5581 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5641 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5701 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5761 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5821 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     5881 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5941 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6001 gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa
     6061 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     6121 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     6181 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg
     6241 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     6301 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6361 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6421 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     6481 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
     6541 tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     6601 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6661 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6721 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6781 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7501 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7561 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7621 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7681 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7741 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7801 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7861 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7921 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7981 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8041 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8101 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8161 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8221 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8281 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8341 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8401 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
//
